Ciliary neurotrophic factor activation of astrocytes mediates neuronal damage via the IL‑6/IL‑6R pathway.

The occurrence of epilepsy is a spontaneous and recurring process due to abnormal neuronal firing in the brain. Epilepsy is understood to be caused by an imbalance between excitatory and inhibitory neurotransmitters in the neural network. The close association between astrocytes and synapses can regulate the excitability of neurons through the clearance of neurotransmitters.

Anion Substitution to Suppress the Voltage Hysteresis of NaMnTi(PO) as a Cathode Material for Sodium-Ion Batteries.

The Mn-based polyanion compound NaMnTi(PO) (NMTP) with a Na superionic conductor (NASICON) structure has attracted incremental attention as a potential cathode material for sodium-ion batteries. However, the occupation of Mn on Na vacancies usually leads to severe voltage hysteresis, which in turn results in significant capacity loss, slow Na diffusion kinetics, and poor cycling stability. Herein, anion-substituted compounds NaMnTi(PO)(SiO) ( = 0.

Ultrasonic imaging of delamination in thick CFRP laminates using an energy-compensation reverse time migration method.

In ultrasonic reflection method, the precision of defect detection in thick carbon fiber reinforced plastics (CFRP) is compromised by acoustic energy attenuation. An energy-compensation reverse time migration (ECRTM) method is proposed to identify multiple defects accurately. Forward and backward wavefields are formed using the finite element method within an anisotropic acoustic model based on the Christoffel equation and Bond transformation.

Boosting Peroxymonosulfate Activation via CoS/MXene Nanocomposite for Rhodamine B Degradation under Simulated Sunlight Irradiation.

Cobalt-based heterogeneous catalysts have been demonstrated as an effective PMS activator for pollutant degradation. However, the limited active sites on their surface lead to an unsatisfactory catalytic efficiency. Immobilizing the catalysts on the support material can be a promising modification strategy to solve this problem.

Repeated reserpine treatment induces depressive-like behaviors accompanied with hippocampal impairment and synapse deficit in mice.

Depression remains a significant public health concern, and current animal models of depression are limited in their ability to accurately mimic human depression. However, studying the new development of antidepressants requires the use of progressive animal models. In this study, the mice were exposed to a low dose of reserpine (0.

Stanniocalcin 1 promotes lung metastasis of breast cancer by enhancing EGFR-ERK-S100A4 signaling.

Lung metastasis is the leading cause of breast cancer-related death. The tumor microenvironment contributes to the metastatic colonization of tumor cells in the lungs. Tumor secretory factors are important mediators for the adaptation of cancer cells to foreign microenvironments.

Anti-Photoaging Effects of Nanocomposites of Amphiphilic Chitosan/18β-Glycyrrhetinic Acid.

Improving the transdermal absorption of weakly soluble drugs for topical use can help to prevent and treat skin photoaging. Nanocrystals of 18β-glycyrrhetinic acid (i.e.

[Establishment of a THP-1 macrophage model infected by recombinant Mycobacterium smegmatis expressing green fluorescent protein].

Objective To establish a THP-1 macrophage model infected by Mycobacterium smegmatis expressing green fluorescent protein (GFP), to quickly locate and visually detect Mycobacterium smegmatis, and to provide a tracer tool to identify the pathogenesis of tuberculosis and develop new tuberculosis vaccines. Methods The enhanced green fluorescent protein (EGFP) gene sequence was amplified by PCR using pEGFP-N1 plasmid as a template to obtain the coding gene of EGFP, and the amplified product was cloned into the vector pALACE to establish the recombinant plasmid pALACE-EGFP. Electroporation transformed the pALACE-EGFP into Mycobacterium smegmatis, and recombinant Mycobacterium smegmatis clones were screened by hygromycin resistance.

[IL-6 promotes the activation of rat astrocytes and down-regulation of the expression of Kir4.1 channel].

Objective To identify the effects of interleukin-6 (IL-6) on astrocytes activation, and the regulation of the expression of inwardly rectifying potassium 4.1 (Kir4.1) channels in astrocytes.

Iridoid Glycosides from Phlomis Medicinalis Diels: Optimized Extraction and Hemostasis Evaluation.

Phlomis medicinalis Diels, an important perennial herbal plant unique to the Qinghai-Tibet Plateau, is often used as Tibetan Materia Medicine Radix Phlomii for the treatment of cold, cough, and convergence trauma. In order to efficiently extract the iridoid glycosides from P. medicinalis, an ultrasound-assisted deep eutectic solvent extraction technique was employed.

Optimized preparation of gastric acid-response sulfhydryl functionalized chitosan/alginate/tilapia peptide hydrogel and its protective effects on alcohol-induced liver and brain injury.

Long-term alcohol intake or drinking large quantities of alcohol at one time can cause organ damage, which in turn can lead to chronic diseases. It is of important clinical and social significance to find effective approaches for the prevention and treatment of alcohol-induced diseases. In this paper, sulfhydryl functionalized chitosan (chitosan--acetyl-l-cysteine, CS-NAC) and sodium alginate (SA) were used as the matrix materials to contain tilapia peptide (TP), and a gastric acid-response hydrogel (CS-NAC/SA/TP) was prepared.

Gastric acid-response chitosan/alginate/tilapia collagen peptide composite hydrogel: Protection effects on alcohol-induced gastric mucosal injury.

Long-term excessive alcohol intake can easily lead to gastritis, gastric ulcer, and gastric bleeding. In this paper, the gastric acid-responsive hydrogel of CS-NAC/alginate/tilapia collagen peptide (CS-NAC/ALG/TCP) was developed. Its structure and properties were determined.

Use of 18β-glycyrrhetinic acid nanocrystals to enhance anti-inflammatory activity by improving topical delivery.

18β-Glycyrrhetinic acid (GA) is often topically applied in clinical treatment of inflammatory skin diseases. However, GA has poor solubility in water, which results in poor skin permeability and low bioavailability. Nanocrystallization of drugs can enhance their permeability and improve bioavailability.

[Insulin-like growth factor 1 (IGF-1) promotes phagocytic activity of mouse BV-2 microglial cells via activating PI3K/AKT signaling pathway].

Objective To investigate the effect of insulin-like growth factor 1 (IGF-1) on the phagocytic activity of mouse BV-2 microglial cells. Methods Western blotting was performed to detect the protein levels of IGF-1 and IGF-1 receptor (IGF-1R) in the murine brain after the establishment of acute central nervous system inflammation models by intraperitoneal lipopolysaccharide (LPS) injection (10 mg/kg). The protein level of IGF-1R on BV-2 microglial cells that had been stimulated by 500 ng/mL LPS for 4, 12 and 24 hours was measured by Western blotting.

Catechol functionalized chitosan/active peptide microsphere hydrogel for skin wound healing.

Chitosan-based thermosensitive hydrogels have been widely used in drug delivery and tissue engineering, but their poor bioactivity has limited their further applications. Integral active oyster peptide microspheres (OPM) with an average particle diameter of 3.9 μm were prepared with high encapsulation efficiency (72.

Chitosan-Based Thermo-Sensitive Hydrogel Loading Oyster Peptides for Hemostasis Application.

Uncontrolled massive hemorrhage is one of the principal causes of death in trauma emergencies. By using catechol-modified chitosan (CS-C) as the matrix material and β glycerol phosphate (β-GP) as a thermo-sensitive agent, chitosan-based thermo-sensitive hydrogel loading oyster peptides (CS-C/OP/β-GP) were prepared at physiological temperature. The hemostatic performance of CS-C/OP/β-GP hydrogel was tested in vivo and in vitro, and its biological safety was evaluated.

MCU-dependent negative sorting of miR-4488 to extracellular vesicles enhances angiogenesis and promotes breast cancer metastatic colonization.

Based on Stephen Paget's well-established theory, both cell-autonomous and non-cell-autonomous mechanisms are crucial for metastasis. Although the mitochondrial calcium uniporter (MCU) has been suggested to be involved in breast cancer (BC) progression via cell-autonomous mechanisms, whether it assists the metastasis of BC cells through non-cell-autonomous mechanisms remains unclear. This study aimed to demonstrate that the MCU regulates BC metastatic colonization via non-cell-autonomous mechanisms.

Marine collagen peptide grafted carboxymethyl chitosan: Optimization preparation and coagulation evaluation.

Uncontrolled bleeding has always been a sudden accident, which is the main cause of casualties in war trauma, emergency events and surgical operations. Rapid hemostatic materials can effectively reduce casualties and save lives. In this paper, marine collagen peptide grafted carboxymethyl chitosan (CMCS-MCP) was synthesized by 1-ethyl-(dimethylaminopropyl) carbodiimide (EDC)-mediated coupling reaction.

Calcium-sensing stromal interaction molecule 2 upregulates nuclear factor of activated T cells 1 and transforming growth factor-β signaling to promote breast cancer metastasis.

Background: Stromal interaction molecule (STIM) 2 is a key calcium-sensing molecule that regulates the stabilization of calcium ions (Ca) and therefore regulates downstream Ca-associated signaling and cellular events. We hypothesized that STIM2 regulates epithelial-mesenchymal transition (EMT) to promote breast cancer metastasis.

Methods: We determined the effects of gain, loss, and rescue of STIM2 on cellular motility, levels of EMT-related proteins, and secretion of transforming growth factor-β (TGF-β).

Sponges of Carboxymethyl Chitosan Grafted with Collagen Peptides for Wound Healing.

Burns are physically debilitating and potentially fatal injuries. Two marine biomaterials, carboxymethyl chitosan (CMC) and collagen peptides (COP), have emerged as promising burn dressings. In this paper, sponges of carboxymethyl chitosan grafted with collagen peptide (CMC-COP) were prepared by covalent coupling and freeze drying.

Knockdown of FAM64A suppresses proliferation and migration of breast cancer cells.

Background: FAM64A is a mitotic regulator promoting cell metaphase-anaphase transition, and it is frequently reported to be highly expressed in cancer cells. However, the role of FAM64A in human breast cancer (BrC) is poorly studied.

Methods: The expression of FAM64A mRNA in BrC samples was determined by RT-qPCR assay and TCGA database mining.

Investigation of the Effects of Molecular Parameters on the Hemostatic Properties of Chitosan.

Hemorrhea is one of the major problems in war, trauma care, and surgical operation that threaten the life of the injured and patients. As a novel polymeric hemostatic agent, biodegradable chitosan can stop bleeding through a variety of approaches. In this paper, chitosan with various molecular parameters was prepared from chitin as raw material through deacetylation, oxidative degradation, hydrophilic modification, and salt formation reactions.

Chitosan-Based Composite Materials for Prospective Hemostatic Applications.

Effective hemostasis is vital to reduce the pain and mortality of patients, and the research and development of hemostatic materials are prerequisite for effective hemostasis. Chitosan (CS), with good biodegradability, biocompatibility and non-toxicity, has been widely applied in bio-medicine, the chemical industry, the food industry and cosmetics. The excellent hemostatic properties of CS have been extensively studied.

Exosomes derived from breast cancer lung metastasis subpopulations promote tumor self-seeding.

Lung metastasis is a primary obstacle in the clinical treatment of metastatic breast cancer. Most patients with lung metastasis eventually die of recurrence. Recurrence may be related to self-seeding, which occurs when circulating tumor cells re-seed into the tumors they originated from (metastasis or carcinoma in situ).

Determination of the field strength and realization of the high-field anodization of aluminum.

Previous studies suggested that high-field anodization of aluminum can be realized given that aluminum is anodized at a high voltage just below the breakdown value. However, increasing the applied voltage cannot guarantee the enhancement in electric field strength across the barrier layer of porous anodic alumina (PAA) due to a concurrent increase of the barrier-layer thickness. Here, we report comparative studies of aluminum anodization in a highly concentrated (0.