Astaxanthin Alleviates Oxidative Stress in Mouse Preantral Follicles and Enhances Follicular Development Through the AMPK Signaling Pathway.

This study investigates the effects of astaxanthin on oxidative stress, mitochondrial function, and follicular development in mouse preantral follicles, with a focus on the involvement of the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway. Astaxanthin (2.5 nM) significantly enhanced both the antrum formation (from 85.

Ionic Liquid-Based Polarity-Adjustable Deep Eutectic Solvent Extraction Followed by High-Performance Liquid Chromatography-Diode-Array Detection for the Determination of Liposoluble Anthraquinones in Salvia miltiorrhiza Bge. Root.

A simple, green, and efficient ultrasound-assisted deep eutectic solvents (DESs) extraction followed by high-performance liquid chromatography-diode array detector was established to detect four liposoluble anthraquinones in Salvia miltiorrhiza Bge. root. In this study, a series of polarity-adjustable DESs were prepared using n-hexanol and tetrabutylammonium chloride, and their structure, density, viscosity, pH, and thermal stability were determined.

Assessing the effectiveness of the varicella vaccine in primary and secondary school students in Qingdao, China: A matched case-control study.

Objectives: The effectiveness of varicella vaccination strategies needs to be further evaluated. The vaccine effectiveness (VE) of the varicella vaccine was evaluated, and changes in VE of varicella vaccination in primary and secondary school students in Qingdao, China, over time were analyzed.

Patients And Methods: We conducted a 1:3 matched case-control study, using data from the Chinese Information System for Disease Control and Prevention, Qingdao Student Health Examination Management System, and Qingdao Immunization Program Information System.

One-Pot Synthesis of Hydrophobic Porphyrin Zirconium-Based MOFs for the Photoreduction of CO to Formate.

Using zirconium tetrachloride as a metal source and tetra(4-carboxyphenyl)porphyrin as a ligand and by in situ introducing octadecylphosphonic acid (OPA), three hydrophobic porphyrin zirconium metal-organic frameworks (MOFs) with different structural topologies were constructed, where these MOFs are labeled as OPA@PCN-222, OPA@PCN-223, and OPA@PCN-224. Compared with the original Zr-MOFs without the modification of OPA, the modified porphyrin Zr-MOFs show excellent hydrophobic properties and can maintain excellent stability in a long-term humidity environment. Meanwhile, OPA@PCN-222, OPA@PCN-223, and OPA@PCN-224 exhibit wide absorption of visible light and steadfast and expeditious photocurrent response by leveraging the properties of porphyrin ligands.

An integrated analysis strategy for characterization of chromones and coumarins from Saposhnikovia divaricata (Turcz.) Schischk. by UHPLC-QTOF-MS.

This study aimed to employ a comprehensive data screening strategy to identify the chromones and coumarins present in Saposhnikovia divaricata (SD). Initially, the five-point mass defect filter (MDF) method was utilized to screen the respective three subclasses of chromones and coumarins for MS. In comparison to the traditional MDF method, the number of interference peaks was reduced from 3462 to 1053, representing a decrease of 69.

Siderophore-Mediated Cooperation in Anammox Consortia.

It has been widely accepted that iron plays an important role in stimulating the activity of anammox bacteria, which contain many iron clusters for electron transport in cells. However, whether anammox bacteria could directly use and how to uptake Fe(III) have been long-time ignored. Here, we found that micrometer-scale magnetite with the size of 10-20 μm significantly promoted the anammox bacterial activity by iron core and iron uptake.

Labile Carbon Input Mitigates the Negative Legacy Effects of Nitrogen Addition on Arbuscular Mycorrhizal Symbiosis in a Temperate Grassland.

Nitrogen (N) deposition and carbon (C) addition significantly influence the dynamics of plant-microbe interactions, particularly altering the symbiotic relationship between plants and arbuscular mycorrhizal fungi (AMF). However, the effects and underlying mechanisms of labile C input on the relationship between AMF and various plant species in a nitrogen-enriched environment remain a knowledge gap. A seven-year field experiment was conducted to examine how six levels of N and three levels of labile C addition impact AMF colonization in four key plant species: (Trin.

Interfacial creation of positively charged sites in LaPO/Fe(PO) heterojunctions for high-current-density oxygen evolution.

The creation of positively charged active sites is crucial for enhancing the adsorption of negatively charged hydroxide ions, thereby improving the sluggish oxygen evolution reaction (OER). We engineered LaPO/Fe(PO) heterojunctions with a low flat-band potential, facilitating interfacial charge transfer and generation of positively charged holes, resulting in a highly active and stable OER catalyst at 500 mA cm.

Clinical features and prognostic predictors of C. Psittaci Pneumonia: a systematic review and individual patient meta-analysis.

Background: The clinical presentations and prognostic indicators of C. psittaci pneumonia are inadequately investigated currently. The objective of the study was to assess the clinical presentation of C.

Artificial intelligence models assisting physicians in quantifying pancreatic necrosis in acute pancreatitis.

Background: Acute pancreatitis (AP) is a potentially life-threatening condition characterized by inflammation of the pancreas, which can lead to complications such as pancreatic necrosis. The modified computed tomography severity index (MCTSI) is a widely used tool for assessing the severity of AP, particularly the extent of pancreatic necrosis. The accurate and timely assessment of the necrosis volume is crucial in guiding treatment decisions and improving patient outcomes.

Readiness for hospital discharge among patients requiring home nutrition support: a cross-sectional study.

Background And Objectives: This study aimed to investigate readiness for hospital discharge of patients requiring home nutrition support and explore the factors that influence this readiness.

Methods And Study Design: This cross-sectional survey included 220 patients discharged from the general surgery department of a tertiary-care teaching hospital in China with home nutrition support. Readiness for Hospital Discharge Scale and Quality of Discharge Teaching Scale scores were calculated and general, disease- and therapy-related information were collected.

Systematic Characterization of the Chemical Constituents of in Vitro and in Vivo of Astragali Radix-Salvia miltiorrhiza Herb Pair by Ultra-High-Performance-Liquid Chromatography-Orbitrap Mass Spectrometry.

Rationale: Astragali radix-Salvia miltiorrhiza (AR-SM) is an herb pair with good therapeutic effects and is widely used. In this study, the in vitro and in vivo components of AR-SM were quickly classified and identified based on UHPLC-orbital mass spectrometry. This provided a basis for clarifying the bioactive substances after compatibility of AR and SM.

CRISPR/Cas12a with Universal crRNA for Indiscriminate Virus Detection.

Viruses, known for causing widespread biological harm and even extinction, pose significant challenges to public health. Virus detection is crucial for accurate disease diagnosis and preventing the spread of infections. Recently, the outstanding analytical performance of CRISPR/Cas biosensors has shown great potential and they have been considered as augmenting methods for reverse-transcription polymerase chain reaction (RT-PCR), which was the gold standard for nucleic acid detection.

The Contribution of Carbapenem-Resistant Pseudomonas Aeruginosa Isolation to Clinical Outcomes in Hospitalized Patients with Exacerbations of Bronchiectasis: A Retrospective Cohort Study.

Background: The antibiotic resistance of Pseudomonas aeruginosa (PA) is increasingly severe in bronchiectasis patients. However, there is currently a lack of research on the clinical outcomes of carbapenem-resistant PA (CRPA) isolation in hospitalized exacerbations of bronchiectasis (HEB) patients. We investigated the incidence, risk factors, and clinical outcomes of PA and CRPA isolation in HEB patients.

Ginseng glucosyl oleanolate inhibit cervical cancer cell proliferation and angiogenesis via PI3K/AKT/HIF-1α pathway.

Ginseng (Panax ginseng C.A. Meyer) is widely used in several functional foods at present.

Autophagy promotes p72 degradation and capsid disassembly during the early phase of African swine fever virus infection.

During viral infections, autophagy functions as a cell-intrinsic defense mechanism by facilitating the delivery of virions or viral components to the endosomal/lysosomal pathway for degradation. In this study, we report that internalized African swine fever virus (ASFV) virions enter autolysosomes during the early phase of viral infection. Autophagy selectively targets the major capsid protein p72 within the ASFV virion.

MiR-200a-3p Attenuates Neuropathic Pain by Suppressing the Bromodomain-Containing Protein 3-Nuclear Factor-κB Pathway.

MicroRNAs (miRNAs) have key roles in the pathological processes of neuropathic pain. Here, our aim was to elucidate the function of miR-200a-3p as well as its related regulatory mechanism in neuropathic pain. An animal model of neuropathic pain was established by chronic constriction injury (CCI) induction.

Multi-drug resistant Pseudomonas aeruginosa isolation is an independent risk factor for recurrent hemoptysis after bronchial artery embolization in patients with idiopathic bronchiectasis: a retrospective cohort study.

Background: Currently, there is a lack of research on multi-drug resistant Pseudomonas aeruginosa (MDR-PA) isolation in bronchiectasis-related hemoptysis. The aim of this study to analyze the risk factors for recurrent hemoptysis following bronchial artery embolization (BAE) and compare the recurrent hemoptysis-free rates between MDR-PA, non-MDR-PA, and non-PA isolation.

Methods: A retrospective study was performed of patients diagnosed with idiopathic bronchiectasis-related recurrent hemoptysis who underwent BAE at an university-affiliated hospital.

A green approach coupled with molecular dynamics simulations and toxicity assays to infer the mode of action for organophosphate esters.

Organophosphate esters (OPEs) have attracted extensive attention due to their toxic effects on human health and biological systems as plasticizers and flame retardants. This study focused on exploring a green approach to get toxicity mechanisms that used less solvents or organisms. The toxicity values of selected organophosphate esters including tri (2-chloroethyl) phosphate (TCEP), tri (1, 3-dichloro-2-propyl) phosphate (TDCP), tripropyl phosphate (TPrP), tri-n-butyl phosphate (TnBP), and tritolyl Phosphate (TCrP) to Photobacterium phosphoreum were determined.

Hepatocyte-specific NR5A2 deficiency induces pyroptosis and exacerbates non-alcoholic steatohepatitis by downregulating ALDH1B1 expression.

Nonalcoholic steatohepatitis (NASH) is a prevalent chronic disease, yet its exact mechanisms and effective treatments remain elusive. Nuclear receptor subfamily 5 group A member 2 (NR5A2), a transcription factor closely associated with cholesterol metabolism in the liver, has been hindered from comprehensive investigation due to the lethality of NR5A2 loss in cell lines and animal models. To elucidate the role of NR5A2 in NASH, we generated hepatocyte-specific knockout mice for Nr5a2 (Nr5a2) and examined their liver morphology across different age groups under a regular diet.

The antiviral effect and potential mechanism of Houttuynia cordata thunb. (HC) against coxsackievirus A4.

Ethnopharmacological Relevance: Hand, foot, and mouth disease (HFMD) is mainly caused by various of enteroviruses such as enterovirus 71 (EVA71), coxsackievirus A16 (CVA16), CVA6, and CVA10 in infants and children under 5 years old. During the past 5 years, CVA4 has become the dominant pathogen resulting in HFMD in China. However, there are no effective vaccines and antiviral drugs available.