Mach-Zehnder interferometer (MZI) point-of-care system for rapid multiplexed detection of microRNAs in human urine specimens.

MicroRNAs have been identified as promising biomarkers for human diseases. The development of a point-of-care (POC) test for the disease-associated miRNAs would be especially beneficial, since miRNAs are unexpectedly well preserved in various human specimens, including urine. Here, we present the Mach-Zehnder interferometer-miRNA detection system capable of detecting multiple miRNAs in clinical urine samples rapidly and simultaneously in a label-free and real-time manner.

Multiplexed detection and differentiation of the DNA strains for influenza A (H1N1 2009) using a silicon-based microfluidic system.

Pandemic influenza by the swine-origin influenza virus (H1N1 2009) has attracted considerable concern worldwide. A convenient and accurate diagnostic approach that can be deployed at the point of care, such as in a doctor's office or at an airport, is critical for disease control. Here we report the development of a silicon-based microfluidic system for subtype differentiation of the novel H1N1 2009 strain vs.

Electrochemical detection of oligonucleotide by attaching redox probes onto its backbone.

An approach was demonstrated to detect oligonucleotide by attaching redox probes onto its backbone. First, peptide nucleic acid (PNA) with a neutral backbone was immobilized onto a gold (Au) electrode surface as a capture. Second, when the PNA capture hybridized with a target oligonucleotide (a short DNA), an assembly of Au-PNA-DNA formed and phosphate groups were thus brought into the assembly from the DNA's backbone.