Ascorbic acid induces osteoblast differentiation of human suspension mononuclear cells.

Background Aims: Suspension mononuclear cells (MNCs) can be differentiated into osteoblasts with the induction of ascorbic acid and β-glycerophosphate. The aim of this study was to determine the ability of suspension MNCs to differentiate into osteoblasts using ascorbic acid only.

Methods: Suspension MNCs were obtained by a combination of gradient centrifugation and culture selection.