Publications by authors named "Siti Hesti Nurbayanti"
J Immunoassay Immunochem
July 2024
Article Synopsis
- Single Chain Variable Fragment (scFv) can replace monoclonal antibodies for diagnostics, but producing it in hosts has been tough due to issues like protein misfolding.
- This study successfully expressed anti-CHIKV E2 scFv by co-expressing three chaperones to boost protein solubility and used Origami B host for production.
- The resulting scFv showed a significant increase in yield and was effectively used in an electrochemical immunosensor for detecting CHIKV-E2, indicating its potential for various immunoassay applications.
