A new ophthalmic microtrephine.

We present a new instrument designed to facilitate the microtrephination of tissues required in various ophthalmic procedures. This microtrephine is a 21-gauge stainless steel tube, 0.81 mm in diameter and 16 mm long, with a sharpened cutting end and a plastic finger-grip area to facilitate micro-rotation.

New minitrephine makes lacrimal canalicular rehabilitation an office procedure.

A new minitrephine, with a stainless steel tube of 0.81-mm outer diameter, 16-mm length, a plastic hub and handle assembly for manual rotation, Luer lock attachment to either standard or specially designed 3-ml syringe with spring-assisted plunger, and an intraluminal stylet--all disposable--has been designed by us principally for opening a distally occluded lacrimal canaliculus prior to stenting with any desired implant. Local anesthesia via a nasociliary nerve block makes this an office procedure.

A convenient procedure for rapid release of protoplasts from Ustilago maydis.

Filtrates from cultures of Trichoderma harzianum, grown on a liquid mineral salts medium containing vitamin and trace element supplements, 0.5 g/l glucose and 2 g/l of dried residue of ethanol-extracted commercial mushrooms (Agaricus brunnescens), are a good source of enzymes for the release of protoplasts from the sporidia of Ustilago maydis. When concentrated 50-fold by (NH4)2SO4 precipitation, the enzymes liberated 95% of the protoplasts from the sporidia within 40 min.

Surgical correction of blepharoptosis and lower-eyelid retraction in dry-eyed patients.

A Fasanella-Servat upper-eyelid tarsal excision was coupled with reimplantation of the excised specimen into the lower lid between its antitarsal margin and the retractor muscles of the lower lid. The reimplantation of tissues preserved secretory elements in four dry-eyed patients whose upper-lid blepharoptosis and lower-lid retraction were both surgically corrected by this procedure.

One-way flow in the lacrimal drainage system: determinants from the basic sciences.

All life processes must be compatible with known physical laws. While the physiology of the lacrimal pump and valvular system are well known, the physical and histologic aspects related to anatomy remain to be clarified. Maximization of forward-directed capillarity, the operation of Bernoulli's law, the Venturi tube effect, and histologic microciliation are related to the system's basic anatomy and directed toward forward flow only, or, conversely, the avoidance of backflow of nasal secretions toward the conjunctival sac.

Mode of action of the azasteroid antibiotic 15-aza-24 methylene-d-homocholesta-8,14-dien-3 beta-ol in Ustilago maydis.

Ustilago maydis sporidia treated with 0.1 mug of azasterol (15-aza-24-methylene-d-homocholesta-8,14-dien-3beta-ol) per ml appeared branched and vacuolated after 6 h of incubation. Sporidial multiplication, dry weight increase, and synthesis of protein, deoxyribonucleic acid, and ribonucleic acid were only slightly or moderately inhibited during the initial 3 h of incubation.

Effects of miconazole and dodecylimidazole on sterol biosynthesis in Ustilago maydis.

Miconazole at minimal fungitoxic concentrations inhibits ergosterol biosynthesis in sporidia of Ustilago maydis by interference with sterol C14 demethylation. The action is analogous to that of the fungicides triarimol and fenarimol. The fungicide 1-dodecylimidazole at low concentrations (0.

Iontophoretic local anesthesia for conjunctival surgery.

An iontophoretic unit, originally designed to deliver local anesthesia to the tympanic membrane, has been adapted for use on the tarsal conjunctiva before excision of conjunctivolithiasis plaques and other intra- and subconjunctival lesions.

Effects of Ancymidol (a Growth Retardant) and Triarimol (a Fungicide) on the Growth, Sterols, and Gibberellins of Phaseolus vulgaris (L.).

The effect of the two substituted pyrimidines, ancymidol (a growth retardant) and triarimol (a fungicide) on Phaseolus vulgaris was studied. Both compounds retarded shoot and root elongation as well as increases in fresh weight. Both compounds caused production of ethylene-like responses when given in high dosages or when applied shortly after germination.

Injected mixture for hemostasis in dacryocystorhinostomy.

A mixture of epinephrine and hyaluronidase, injected into the naso-orbital recess at the start of dacryocystorhinostomy and into the nasal mucosa immediately after bone penetration, appears to have a greater hemostatic effect than epinephrine alone. Surgical time is shortened by the reduced need for angular vessel ligation, suction, cautery of bleeding, and nasal packing. Postoperative ecchymosis and nasal clotting are also reduced.

Senile ectropion and entropion: a comparative histopathological study.

A microscopic histopathological study was done on 500 full-eyelid-thickness surgical specimens: 25 with the diagnosis of senile ectropion and 25 with that of senile entropion. Five different staining techniques were used. There appears to be significantly more orbicularis and Riolan's muscle ischemia, atrophy, and collagen fragmentation with ectropion than with entropion.

Gene mutation eliminating antimycin A-tolerant electron transport in Ustilago maydis.

A class of mutants of Ustilago maydis selected on a fungitoxic oxathiin lack of antimycin A-tolerant respiratory system which is present in wild-type cells. This system provides, directly or indirectly, for considerable resistance to antimycin A because growth of mutant cells lacking the system is much more sensitive to the antibiotic than that of the wild type. Antimycin A-sensitive O(2) uptake and growth is found in half of the progeny from crosses of mutant to wild type.