Production and application of purified mutanase from novel Cellulosimicrobium funkei SNG1 in the in vitro biofilm degradation.

Mutanase (α-1,3-glucanase) is an inducible extracellular enzyme with potential medical applications in dentistry. A novel Cellulosimicrobium funkei strain SNG1 producing mutanase enzyme using α-1,3-glucans was isolated, and the enzyme was optimized for increased productivity using the one-factor-at-a-time approach. Maximum growth and enzyme-specific activity (2.

A comprehensive review on mutan (a mixed linkage of α-1-3 and α-1-6 glucans) from bacterial sources.

Mutan is an extracellular sticky polymer having α-1-3 and α-1-6 glycosidic linkages with a large diversity in molecular weights and structures depending on the source. These compounds are reported to be highly thermostable and also have potential physiochemical and biological applications. The main aim of this review is to provide an overview of glucosyltransferases and their role in mutan synthesis.