Publications by authors named "Siriporn Nathalang"
and Allele Frequencies Among Southern Thai Blood Donors.
Indian J Hematol Blood Transfus
July 2018
Diego (DI) blood group genotyping is clinically important in Asian populations. Data of Diego blood type among southern Thais is still unknown. This study aimed to report and allele frequencies in southern Thai blood donors and to estimate potential risk of Di incompatibility and alloimmunization in Thai populations.
View Article and Find Full Text PDFBackground: Antigen-negative red cell transfusion is required for transfusion-dependent patients. We developed multiplex PCR for red cell genotyping and calculated the possibility of finding compatible predicted phenotypes in Thai blood donor populations according to red cell alloantibodies found among Thai patients.
Methods: 600 DNA samples obtained from unrelated healthy central and northern Thai blood donors were tested with the newly developed multiplex PCR for , , , , , , and , and allele detections.
Article Synopsis
- The study aimed to create an in-house multiplex-PCR technique for genotyping human neutrophil antigens (HNA) -1, -3, -4, and -5 within the Thai population to assist in diagnosing alloimmune neutropenia and improve granulocyte transfusions.
- 500 DNA samples from healthy Thai blood donors were used for testing, with results from the new multiplex-PCR showing validity and agreement with established PCR methods; rare genotype HNA-4b4b was notably absent.
- Findings indicate that multiplex-PCR is a cost-effective and efficient alternative to traditional methods, making it suitable for routine HNA genotyping, especially in developing countries.
Alloantibodies to the Diego (DI) blood group system, anti-Dia and anti-Dib are clinically significant in causing hemolytic transfusion reactions (HTRs) and hemolytic disease of the fetus and newborn (HDFN), especially in Asian populations with Mongolian ancestry. This study aimed to report the frequency of the DI*A and DI*B alleles in a Central Thai population and to compare them with those of other populations previously published. Altogether, 1,011 blood samples from unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok were included.
View Article and Find Full Text PDFBackground: Duffy (FY) blood group genotyping is important in transfusion medicine because Duffy alloantibodies are associated with delayed hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. In this study, FY allele frequencies in Thai blood donors were determined by in-house PCR with sequence-specific primers (PCR-SSP), and the probability of obtaining compatible blood for alloimmunized patients was assessed.
Methods: Five hundred blood samples from Thai blood donors of the National Blood Centre, Thai Red Cross Society, were included.
Severe transfusion-related acute lung injury (TRALI) is often due to antibodies in blood components directed against human neutrophil antigen (HNA)-3a. This study aimed to report the genotype frequencies of the HNA-3 system and to estimate the potential risk of HNA-3 incompatibility and alloimmunization in two Thai populations. Eight hundred DNA samples obtained from 500 unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok and 300 samples from the Blood Bank, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand were included.
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