Cell-based assay to detect small molecules restoring levels of let-7 miRNAs.

Blockage of miRNA biogenesis by LIN28, or other mechanisms, results in derepression of target genes, some of which are oncogenic (e.g., ) potentially contributing to tumor progression and drug resistance.

Results and lessons from dual extraction of DNA and RNA from formalin-fixed paraffin-embedded breast tumor tissues for a large Cancer epidemiologic study.

Background: The use of archived formalin-fixed paraffin-embedded (FFPE) tumor tissues has become a common practice in clinical and epidemiologic genetic research. Simultaneous extraction of DNA and RNA from FFPE tissues is appealing but can be practically challenging. Here we report our results and lessons learned from processing FFPE breast tumor tissues for a large epidemiologic study.