Inhibition of LncRNA Vof-16 expression promotes nerve regeneration and functional recovery after spinal cord injury.

Our previous RNA sequencing study showed that the long non-coding RNA ischemia-related factor Vof-16 (lncRNA Vof-16) was upregulated after spinal cord injury, but its precise role in spinal cord injury remains unclear. Bioinformatics predictions have indicated that lncRNA Vof-16 may participate in the pathophysiological processes of inflammation and apoptosis. PC12 cells were transfected with a pHBLV-U6-MCS-CMV-ZsGreen-PGK-PURO vector to express an lncRNA Vof-16 knockdown lentivirus and a pHLV-CMVIE-ZsGree-Puro vector to express an lncRNA Vof-16 overexpression lentivirus.

A new griseofulvin derivative from a soft coral-derived fungus sp. SCSIO41208.

A new griseofulvin derivative, eupenigriseofulvin (), together with six known compounds, griseofulvin (), dechlorogriseofluvin (), dechloroisogriseofulvin (), trichopyrone (), 2-(4-hydroxyphenyl)-ethanol (), and 1-phenylethane-1,2-diol (), were isolated from the EtOAc extract of sp. SCSIO41208. The structures of these compounds were elucidated by spectroscopic methods including NMR and mass spectrometry.

Culturable fungal diversity and cellulase production by mixed culture Aspergillus fungi from Sanya mangrove.

Fungi are the most suitable cellulase producers attributing to its ability to produce a complete cellulase system. 33 Genus, 175 Species fungi were isolated from Sanya mangrove, Hainan, China. Using congo red cellulose (CMC) medium, five fungi of cellulose-degrading were selected for further study.

Community Composition and Transcriptional Activity of Ammonia-Oxidizing Prokaryotes of Seagrass in Coral Reef Ecosystems.

Seagrasses in coral reef ecosystems play important ecological roles by enhancing coral reef resilience under ocean acidification. However, seagrass primary productivity is typically constrained by limited nitrogen availability. Ammonia oxidation is an important process conducted by ammonia-oxidizing archaea (AOA) and bacteria (AOB), yet little information is available concerning the community structure and potential activity of seagrass AOA and AOB.

Fungal Community Successions in Rhizosphere Sediment of Seagrasses Enhalus acoroides under PAHs Stress.

Seagrass meadows represent one of the highest productive marine ecosystems and are of great ecological and economic values. Recently, they have been confronted with worldwide decline. Fungi play important roles in sustaining the ecosystem health as degraders of polycyclic aromatic hydrocarbons (PAHs), but fewer studies have been conducted in seagrass ecosystems.

Molecular docking and site-directed mutagenesis of a Bacillus thuringiensis chitinase to improve chitinolytic, synergistic lepidopteran-larvicidal and nematicidal activities.

Bacterial chitinases are useful in the biocontrol of agriculturally important pests and fungal pathogens. However, the utility of naturally occurring bacterial chitinases is often limited by their low enzyme activity. In this study, we constructed mutants of a Bacillus thuringiensis chitinase with enhanced activity based on homology modeling, molecular docking, and the site-directed mutagenesis of target residues to modify spatial positions, steric hindrances, or hydrophilicity/hydrophobicity.

Cellulose nanowhiskers: preparation, characterization and cytotoxicity evaluation.

Objective: To characterize the structure and to evaluate the cytotoxicity of cellulose nanowhiskers (CNW) prepared from cotton linters.

Methods: A series of CNW dispersions was prepared by hydrolyzing cotton linters with sulfuric acid. The structure of CNW was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD).