Insight into the Potential of Somatostatin Vaccination with Goats as a Model: From a Perspective of the Gastrointestinal Microbiota.

Deciphering the gastrointestinal microbial response to oral SS DNA vaccines with different doses is helpful for identifying the mechanism for effective utilization of the vaccine for improving animal production. Here, we conduct a comparative study with different doses of vaccine (control: empty plasmid; low dose: 1 × 10 CFU vaccine; high dose: 1 × 10 CFU vaccine) using goat as a case to investigate the potential of somatostatin vaccination from the entire gastrointestinal microbiota perspective. Our results show that body weight gain and slaughter rate are greater in the L_SS group than in the C_SS group.

Risk of Cytomegalovirus Viremia Following Transplantation of Hepatitis C-Viremic Donor Kidneys Into Uninfected Recipients: A Multi-Center Retrospective Cohort Study.

Background: Several studies have suggested an increased risk of cytomegalovirus (CMV) viremia among Hepatitis C virus (HCV)-uninfected recipients of kidney transplants from HCV-RNA+ deceased donors (HCV D+/R-), but these studies featured small sample sizes and limited ability to address confounding variables.

Methods: We assembled a retrospective cohort of adult kidney transplant recipients at five US centers between 4/1/2015 and 12/31/2020 to determine the association between HCV D+/R- transplants and the outcomes of CMV viremia (> 1000 IU/mL), death-censored graft failure, and mortality in the first posttransplant year compared to HCV D-/R- transplants. We generated highly similar matched cohorts of HCV D+/R- and HCV D-/R- recipients based on attributes that affect the risk of CMV viremia.

Equity of National Essential Public Health Services (NEPHS) in Mainland China, 2019: a cross-sectional study based on the NEPHS database.

Background: China's National Essential Public Health Service (NEPHS) Program was launched in 2009 to deliver population-based public health and individual health management services to all residents at 800,000 primary health-care centers nationwide. This study assessed NEPHS utilization data and evaluated usage inequities using comprehensive nationwide data.

Methods: A cross-sectional study was conducted, selecting 16 indicators (out of 18) from 12 service packages to evaluate inequity.

Three-Dimensional Electrode Model for EEG Forward Problem.

Objective: This study aims to address inaccuracies in model description and boundary representation due to dimensionality loss in the commonly-used point electrode model (PEM) and complete electrode model (CEM) for EEG forward problem (FP).

Methods: To overcome these limitations, this paper proposes a three-dimensional electrode model (TEM). Firstly, we devise an extension-constraint framework to generate an electrode mesh based on the structural priors and seamlessly integrate it into the MRI-based head mesh through two modules: (1) an extension module designed to extend a cuboid mesh containing both head and electrode meshes to avoid the challenges in fusing two irregular stepped surface mesh, and (2) loosely coupled constraints formulated in the constraint module to describe the electrode structure and enhance the flexibility and extensibility.

Retinal Protective Effect of Mono-Ethyl Fumarate in Experimental Age-Related Macular Degeneration via Anti-Oxidative and Anti-Apoptotic Alterations.

Age-related macular degeneration (AMD) is a leading cause of vision impairment in people over the age of 60. Currently, the FDA-approved drugs for AMD have various side effects, and there is a notable lack of drug development for dry AMD. This study aimed to explore the therapeutic effects of mono-ethyl fumarate (MEF) on AMD.

[Fontanel compensation for infant electroencephalography forward modeling method].

Magnetic resonance imaging (MRI)-based electroencephalography (EEG) forward modeling method has become prevalent in the field of EEG. However, due to the inability to obtain clear images of an infant's fontanel through MRI, the fontanelle information is often lacking in the EEG forward model, which affects accuracy of modeling in infants. To address this issue, we propose a novel method to achieve fontanel compensation for infant EEG forward modeling method.

Monitoring of Pathogens Carried by Imported Flies and Cockroaches at Shenzhen Ports.

This study tested the efficacy of xenomonitoring using contaminated flies and cockroaches at ports in Shenzhen by analysing sample data from imported flies and cockroaches from October 2023 to April 2024 to identify the pathogens they carried. Among all the samples of flies and cockroaches collected, and accounted for the highest proportion, 27.59% and 66.

Targeted Delivery of α-ketoglutarate to Macrophages in Bone: A Novel Therapeutic Strategy for Improving Fracture Healing in Type 2 Diabetes.

Impaired fracture healing is a common complication in type 2 diabetes mellitus (T2DM), with limited effective treatments. This study investigates the role of macrophages in bone repair and introduces a novel therapeutic strategy. Reduced glutaminase (GLS) expression and glutaminolysis are found in macrophages from T2DM mice and monocytes from T2DM patients.

The association between a history of induced abortion for nonmedical reasons and maternal and neonatal perinatal outcomes: a retrospective cohort study.

Introduction: Following the implementation of the universal two-child policy in China, many multiparous women who had a history of induced abortion gave birth again. However, there is a lack of studies exploring the associations between induced abortion for nonmedical reasons and maternal and neonatal perinatal complications.

Methods: In this retrospective cohort study, the participants were multiparous women who gave birth to singleton babies at or after 28 weeks of gestation between 1 December 2015, and 1 December 2020.

The moderating effect of working years on nurses' medical narrative ability and empathy ability: a cross-sectional survey study.

Objective: To explore the moderating effect of working years on nurses' medical narrative ability and empathy ability.

Methods: A multi-center cross-sectional survey was conducted among 1920 clinical nurses from 12 general hospitals of different levels in 7 cities of Hunan Province. Data were collected using a general information questionnaire, Narrative Competence Scale (NCS), and the Jefferson Empathy Health Professional Scale (JSE-HP).

RNA neoantigen vaccines prime long-lived CD8 T cells in pancreatic cancer.

A fundamental challenge for cancer vaccines is to generate long-lived functional T cells that are specific for tumour antigens. Here we find that mRNA-lipoplex vaccines against somatic mutation-derived neoantigens may solve this challenge in pancreatic ductal adenocarcinoma (PDAC), a lethal cancer with few mutations. At an extended 3.

Polyploidization leads to salt stress resilience via ethylene signaling in citrus plants.

Polyploidization is a common occurrence in the evolutionary history of flowering plants, significantly contributing to their adaptability and diversity. However, the molecular mechanisms behind these adaptive advantages are not well understood. Through comprehensive phenotyping of diploid and tetraploid clones from Citrus and Poncirus genera, we discovered that genome doubling significantly enhances salt stress resilience.

Short-Term Associations Between Ambient Ozone and Acute Myocardial Infarction Onset Among Younger Patients: Results From the VIRGO Study.

The association between ambient ozone (O) and acute myocardial infarction (AMI) onset is unclear, particularly for younger patients and AMI subtypes. This study examined the short-term association of O with AMI onset in patients aged 18-55 years and explored differences by AMI subtypes and patient characteristics. We analyzed 2,322 AMI patients admitted to 103 US hospitals (2008-2012).

Injectable pH Responsive Conductive Hydrogel for Intelligent Delivery of Metformin and Exosomes to Enhance Cardiac Repair after Myocardial Ischemia-Reperfusion Injury.

Myocardial ischemia-reperfusion injury (MIRI) is a leading cause of complications and high mortality associated with acute myocardial infarction. Injectable hydrogel emerges as a promising biomaterial for myocardial repair due to their ability to mimic the mechanical and electrophysiological properties of heart tissue. In this study, an injectable conductive hydrogel is developed that responds to the weakly acidic microenvironment of ischemic injury, enabling the intelligent release of metformin and exosomes to enhance cardiac repair following MIRI.

Engineering for Biosynthesis of 3'-Hydroxygenistein.

The polyhydroxy isoflavone 3'-hydroxygenistein (3'-OHG) has a wide range of pharmaceutical and nutraceutical benefits. Therefore, it is important to develop an efficient and sustainable method for 3'-OHG production. Here, we engineered the metabolic pathways of to achieve biosynthesis of 3'-OHG.

Synthesis of Cationic Cyclic Oligo(disulfide)s via Cyclo-Depolymerization: A Redox-Responsive and Potent Antibacterial Reagent.

Antimicrobial peptides (AMPs) and synthetic topologically defined peptide mimics have been developed as alternatives to traditional small-molecule antibiotics. AMP mimetics arising from linear polymers used widely in preclinical studies have shown promise but have limited stability. Oligomers possessing cyclic topology have been proposed to have increased stability but remain understudied due to synthetic challenges and concerns over cytotoxicity.

Performance Assessment of All-Solid-Waste High-Strength Concrete Prepared from Waste Rock Aggregates.

In order to solve the problems of the large-scale resource utilization of iron ore waste rock, waste rock is used to prepare green building materials, but it needs to be further promoted for use in high-strength concrete. In this study, high-strength concrete was prepared using iron ore waste rock as coarse and fine aggregates combined with solid waste-based cementitious materials. The mechanical and durability properties of washed and unwashed concrete with two types of aggregates were compared, including compressive strength, freeze resistance, chloride ion resistance, carbonation resistance, pore distribution, microstructural characteristics, and environmental and economic benefits.

Identification and evaluation of blood transcriptional biomarker for tuberculosis screening.

Objectives: Non-sputum-based screening methods for active case finding are a priority for ending tuberculosis. We sought to identify and evaluate blood transcriptional biomarkers suitable for tuberculosis screening.

Methods: We integrated five blood RNA-seq datasets from global tuberculosis patients and identified genes that are differentially expressed between tuberculosis patients and healthy controls, using resampling and exhaustive testing.

Hybrid PET/MRI Imaging of 18F-Fluorodeoxyglucose and 18-kDa Translocator Protein for Presurgical Localization in Refractory Epilepsy.

Purpose: Surgery remains the only curative option for a third of refractory epilepsy patients, though success depends on precise localization of the epileptogenic zone (EZ). This study aims to assess the clinical value of hybrid F-FDG and F-DPA-714 PET/MRI for accurate localization and precise boundary delineation.

Methods: The refractory epilepsy patients who underwent surgery at Xuanwu Hospital from November 2022 to November 2023 were retrospectively recruited.

Synthesis and Bioactivity of Selenium Nanoparticles From Tussilago farfara L. Polysaccharides: Antioxidant Properties and MCF-7 Cell Inhibition.

The present study reports the synthesis of selenium nanocomplexes (Se-TFPs) using purified polysaccharides from Tussilago farfara L. (coltsfoot). It evaluates its structural characteristics, physicochemical properties, and inhibitory effects of Michigan Cancer Foundation-7 (MCF-7) breast cancer cells.

Opportunities and challenges of immuno-oncology: A bibliometric analysis from 2014 to 2023.

The emergence of immuno-oncology (IO) has led to revolutionary changes in the field of cancer treatment. Despite notable advancements in this field, a thorough exploration of its full depth and extent has yet to be performed. This study provides a comprehensive overview of publications pertaining to IO.

Assessment of cardiac iron deposition and genotypic classification in pediatric beta-thalassemia major: the role of cardiac MRI.

Background: Beta thalassemia major (β-TM) is a severe genetic anemia with considerable phenotypic heterogeneity. This study investigated whether genotype correlates with distinct myocardial iron overload patterns, assessed by cardiovascular magnetic resonance (CMR) T2* values.

Methods: CMR data for cardiac iron deposition evaluation, which recruited pediatric participants between January 2021 and December 2024, were analyzed with CVI42.

Clinical outcomes and care for spontaneous bacterial peritonitis: A national cohort study.

Background And Aims: Spontaneous bacterial peritonitis (SBP) leads to high rates of acute kidney injury (AKI), hepatorenal syndrome, and mortality. Population-based studies on contemporary SBP epidemiology are needed to inform care. In a large, national cohort of patients diagnosed with SBP and confirmed by ascitic fluid criteria, we characterized ascitic fluid characteristics, in-hospital and 12-month mortality, AKI, and recurrent SBP.