Light-Blocking Nanofiber Membranes Facilitating Physiologically Relevant In Situ Transmigration Assay.

Nanofiber (NF) membranes have demonstrated considerable potential in cellular transmigration studies due to their resemblance to the biophysical properties of basement membranes, enabling cellular behaviors that closely mimic those observed in vivo. Despite their advantages, conventional NF membranes often encounter issues in transmigration assays due to their transparency, which leads to overlapping fluorescent signals from transmigrated and nontransmigrated cells. This overlap complicates the clear differentiation between these cell populations, making the quantitative evaluation of live-cell transmigration challenging.