Inflammatory cytokines reaction elicited by root-end filling materials.

Cytokines are inflammation in the list of tissue reactions that cytokines control of cell and tissue growth, development, and differentiation. Root-end filling materials often contact with existing periapical tissue, and they need to be biocompatible with remnant periapical tissue. The aim of this study was to focus the effects of the root end filling materials on bone cell viability and expression of inflammatory cytokines and their role in maintaining health and stability of the restored dental tissues.

Biocompatibility of human osteosarcoma cells to root end filling materials.

Ideal root end filling materials should have good physical and chemical properties, and the most important is that the material should be biocompatible with periradicular tissue. The biocompatibility of three root end filling materials, mineral trioxide aggregate, calcium hydroxide-based cement, and eugenol-based cement, were investigated in vitro by culturing extracts of these materials with human osteogenic sarcoma cells (U2OS). Extracts of each of the materials were made after incubation of the materials for 1 day and 1 week with complete McCoy's medium.