Comparison of anti-tumor efficacy of paclitaxel delivered in nano- and microparticles.

This research compares the anti-tumor efficacy of paclitaxel delivered intratumorally in PLGA nanoparticles, microparticles, or the commercial Paclitaxel Injection((R)). The hypothesis of the research is that larger PLGA microparticles adhere to mucus on the cell surface, release paclitaxel locally, and enhance cellular association of paclitaxel. PLGA-paclitaxel particles of mean diameters 315 nm, 1 microm, and 10 microm were prepared and their drug content, in vitro release, and cellular association of paclitaxel into 4T1 cells quantified.

Effect of particle size of nanospheres and microspheres on the cellular-association and cytotoxicity of paclitaxel in 4T1 cells.

Purpose: To compare the effect of size of delivery systems on the cell-association and in vitro cytotoxicity of paclitaxel.

Methods: Four sizes of PLGA-paclitaxel particles were prepared to study the effect of particle size on the cell-association of paclitaxel in 4T1 monolayer in the presence, and absence, of BCRP inhibitor, endocytic inhibitor, and P-glycoprotein (P-gp) inhibitor. Paclitaxel cell-association studies were repeated in Caco-2, Cor-L23/R, and bovine brain microvessel endothelial cells (BBMECs), as well as the association of etoposide in 4T1 cells.

Particle size and temperature effect on the physical stability of PLGA nanospheres and microspheres containing Bodipy.

The purpose of this study was to investigate the effect of particle size, storage temperature, and duration of storage on the physical stability and morphology of polylactic-co-glycolic acid (PLGA) nanospheres and microspheres. PLGA nanospheres and microspheres containing the fluorescent dye, Bodipy, were prepared in varying sizes by controlling the method and degree of agitation during the emulsification phase of preparation. Mean diameters of the particles were measured by dynamic light scattering.

Polymer relationships during preparation of chitosan-alginate and poly-l-lysine-alginate nanospheres.

The preparation of chitosan-alginate nanospheres is described and their properties compared to the poly-L-lysine-alginate system. The mass ratio range of sodium alginate:CaCl(2):cationic polymer (poly-L-lysine [PLL] or chitosan) to prepare nanospheres was 100:17:10. This mass ratio ensured that the calcium alginate was maintained in the pre-gel phase and sufficient cationic polymer was present to form nanospheres.