Loss-of-function mutations in Dnmt3a and Tet2 lead to accelerated atherosclerosis and concordant macrophage phenotypes.

Clonal hematopoiesis of indeterminate potential (CHIP) is defined by the presence of a cancer-associated somatic mutation in white blood cells in the absence of overt hematological malignancy. It arises most commonly from loss-of-function mutations in the epigenetic regulators DNMT3A and TET2. CHIP predisposes to both hematological malignancies and atherosclerotic cardiovascular disease in humans.

Climate change will reduce North American inland wetland areas and disrupt their seasonal regimes.

Climate change can alter wetland extent and function, but such impacts are perplexing. Here, changes in wetland characteristics over North America from 25° to 53° North are projected under two climate scenarios using a state-of-the-science Earth system model. At the continental scale, annual wetland area decreases by ~10% (6%-14%) under the high emission scenario, but spatiotemporal changes vary, reaching up to ±50%.

and gene polymorphism-a possible association with drug-resistant tuberculosis in the north Indian population.

Objectives: The objective of this study is to analyze the association between and gene polymorphism with drug resistant tuberculosis (PTB, MDR-TB, and XDR-TB) in a population from Agra, Uttar Pradesh.

Methods: The present case-control study included 101 pulmonary TB patients, 104 multidrug-resistant TB patients, 48 extremely drug-resistant TB patients, and 130 healthy and unrelated controls residing in the same locality. The genotyping method for was carried out by allele-specific polymerase chain reaction (PCR), and gene polymorphism was performed by hybridization probe chemistry in Roche Real-Time PCR.

Investigation of In Vitro Anti-cancer and Apoptotic Potential of Onion-Derived Nanovesicles Against Prostate and Cervical Cancer Cell Lines.

Plant-derived compounds have recently garnered significant interest in the field of medicine due to their rich repertoire of phytochemicals, which holds promise for exploring novel therapies to treat cancer. This study embarks on the first-time investigation of the anti-cancerous effect of onion-derived nanovesicles (ODNVs). ODNVs were isolated employing differential centrifugation followed by ultracentrifugation and subsequent characterization using dynamic light scattering (DLS), field emission scanning electron microscopy (FESEM), and Fourier transform infrared spectroscopy (FTIR).

Pan msr gene deleted strain of Salmonella Typhimurium suffers oxidative stress, depicts macromolecular damage and attenuated virulence.

Salmonella encounters but survives host inflammatory response. To defend host-generated oxidants, Salmonella encodes primary antioxidants and protein repair enzymes. Methionine (Met) residues are highly prone to oxidation and convert into methionine sulfoxide (Met-SO) which compromises protein functions and subsequently cellular survival.

Many Cooks in the Kitchen: Iterating a Qualitative Analysis Process Across Multiple Countries, Sites, and Teams.

Establishing and proving methodological rigor has long been a challenge for qualitative researchers where quantitative methods prevail, but much published literature on qualitative analysis assumes a relatively small number of researchers working in relative proximity. This is particularly true for research conducted with a grounded theory approach. Different versions of grounded theory are commonly used, but this methodology was originally developed for a single researcher collecting and analyzing data in isolation.

Sustainable Nanoparticles from Stephania glabra and Analysis of Their Anticancer Potential on 2D and 3D Models of Prostate Cancer.

In pursuit of a novel effective treatment for prostate cancer, methanolic extract of Stephania glabra tubers (Sg-ME) was utilized to fabricate silver (Sg-AgNP), copper oxide (Sg-CuONP), and silver-copper bimetallic nanoparticles (Sg-BNP). The characterization of the nanoparticles confirmed spherical shape with average diameters of 30.72, 32.

Paired bone marrow and peripheral blood samples demonstrate lack of widespread dissemination of some CH clones.

Clonal hematopoiesis (CH) represents clonal expansion of mutated hematopoietic stem cells detectable in the peripheral blood or bone marrow through next generation sequencing. The current prevailing model posits that CH mutations detected in the peripheral blood mirror bone marrow mutations with clones widely disseminated across hematopoietic compartments. We sought to test the hypothesis that all clones are disseminated throughout hematopoietic tissues by comparing CH in hip vs peripheral blood specimens collected at the time of hip replacement surgery.

India's Riverine Nitrogen Runoff Strongly Impacted by Monsoon Variability.

Agricultural intensification in India has increased nitrogen pollution, leading to water quality impairments. The fate of reactive nitrogen applied to the land is largely unknown, however. Long-term records of riverine nitrogen fluxes are nonexistent and drivers of variability remain unexamined, limiting the development of nitrogen management strategies.

Systematic review and meta-analysis of human Toll-like receptors genetic polymorphisms for susceptibility to tuberculosis infection.

Epidemiological data from the world health organization (WHO) show that Globally an estimated 10 million (range, 8.9-11.0 million) people around the world were infected with TB in 2019.

Mutant - and -Driven Hematopoiesis Populates the Hematopoietic Compartment in Response to Peptide Receptor Radionuclide Therapy.

Purpose: Hematologic toxic effects of peptide receptor radionuclide therapy (PRRT) can be permanent. Patients with underlying clonal hematopoiesis (CH) may be more inclined to develop hematologic toxicity after PRRT. However, this association remains understudied.

Implication of imposing fertilizer limitations on energy, agriculture, and land systems.

Since the 1950's, global fertilizer usage has increased by more than 800% resulting in detrimental impacts to the environment. The projected increase in crop production due to increasing demands for food, feed, biofuel, and other uses, may further increase fertilizer usage. Studies have examined achieving agricultural intensification in environmentally sustainable ways, however, they have not focused on the whole-system economic aspects of changes in fertilizer usage over the long term.

Exploring the Role of C-C Motif Chemokine Ligand-2 Single Nucleotide Polymorphism in Pulmonary Tuberculosis: A Genetic Association Study from North India.

The C-C motif chemokine ligand-2 (CCL2) was evidenced to be associated with tuberculosis susceptibility in some ethnic groups. In the present study, effort was made to find out the association of -2518 A>G and -362 G>C variants with susceptibility to TB in a population from North India. The genotyping was carried out in 373 participants with pulmonary TB (PTB) and 248 healthy controls (HCs) for 2518 A>G and -362 G>C polymorphisms by PCR-RFLP and by melting curve analysis using fluorescence-labeled hybridization fluorescent resonance energy transfer (FRET) probes, respectively, followed by DNA sequencing in a few representative samples.

A proof-of-concept of lateral flow based luteinizing hormone detection in urine for ovulation prediction in buffaloes.

We present a method for the detection of luteinizing hormone (LH) in buffalo urine by using gold nanoparticles (AuNPs) conjugated with novel anti-peptide antibodies against LH (anti LHP) in lateral flow assay format. Buffalo LH is an important reproductive hormone and is a chemically complex glycoprotein. Its surge release precedes ovulation and therefore detecting LH has implications in identifying the ovulation event.

Long-Term Changes in Precipitation and Temperature Have Already Impacted Nitrogen Loading.

Increases in nitrogen loading over the past several decades have led to widespread water quality impairments across the U.S. Elevated awareness of the influence of climate variability on nitrogen loading has led to several studies investigating future climate change impacts on water quality.

Societal decisions about climate mitigation will have dramatic impacts on eutrophication in the 21 century.

Excessive nitrogen runoff leads to degraded water quality, harming human and ecosystem health. We examine the impact of changes in land use and land management for six combinations of socioeconomic pathways and climate outcomes, and find that societal choices will substantially impact riverine total nitrogen loading (+54% to -7%) for the continental United States by the end of the century. Regional impacts will be even larger.

Biomarkers of Cardiac Health and Disease.

Cardiovascular diseases (CVDs) are globally associated with high rates of morbidity and mortality. CVDs are diagnosed based on clinical assessment, physical examination, disease history, electrocardiogram, and chest X-ray. However, these parameters have limited sensitivity and specificity.

Data and methods for studying commercial motor vehicle driver fatigue, highway safety and long-term driver health.

This article summarizes the recommendations on data and methodology issues for studying commercial motor vehicle driver fatigue of a National Academies of Sciences, Engineering, and Medicine study. A framework is provided that identifies the various factors affecting driver fatigue and relating driver fatigue to crash risk and long-term driver health. The relevant factors include characteristics of the driver, vehicle, carrier and environment.

Long-Term Phosphorus Loading and Springtime Temperatures Explain Interannual Variability of Hypoxia in a Large Temperate Lake.

Anthropogenic eutrophication has led to the increased occurrence of hypoxia in inland and coastal waters around the globe. While low dissolved oxygen conditions are known to be driven primarily by nutrient loading and water column stratification, the relative importance of these factors and their associated time scales are not well understood. Here, we explore these questions for Lake Erie, a large temperate lake that experiences widespread annual summertime hypoxia.

Eutrophication will increase during the 21st century as a result of precipitation changes.

Eutrophication, or excessive nutrient enrichment, threatens water resources across the globe. We show that climate change-induced precipitation changes alone will substantially increase (19 ± 14%) riverine total nitrogen loading within the continental United States by the end of the century for the "business-as-usual" scenario. The impacts, driven by projected increases in both total and extreme precipitation, will be especially strong for the Northeast and the corn belt of the United States.

Association of Nitric Oxide Synthase2 gene polymorphisms with leprosy reactions in northern Indian population.

The pathogen Mycobacterium leprae causes leprosy that affects mainly skin and nerves. Polymorphisms of certain genes are substantiated to be associated with the susceptibility/resistance to leprosy. The present investigation addressed the association of Nitric Oxide Synthase2 gene polymorphisms and leprosy in a population from northern part of India.

Immunofocusing using conformationally constrained V3 peptide immunogens improves HIV-1 neutralization.

V3-directed antibodies are present in practically all HIV-1 infected patients and in individuals vaccinated with gp120. The levels of maternal V3-directed antibodies were recently shown to correlate with reduced mother to child transmission, and V3 IgGs were found to be a negative correlate of risk in the RV-144 human trial. mAb directed to the tip of the V3 are capable of broad neutralization of Tier-1 and some Tier-2 viruses.