Unearthing novel and multifunctional peptides in peptidome of fermented chhurpi cheese of Indian Himalayan region.

Fermented foods of the Indian Himalaya are unexplored functional resources with high nutritional potential. Chhurpi cheese, fermented by defined native proteolytic lactic acid bacteria of Sikkim was assessed for ACE inhibitory, HOCl reducing, and MPO inhibitory, activity across varying stages of gastrointestinal (GI) digestion. The enhanced bioactivity of Lactobacillus delbrueckii WS4 chhurpi was associated with the generation of bioactive and multifunctional peptides during fermentation and GI digestion.

Comparative assessment of empirical and hybrid machine learning models for estimating daily reference evapotranspiration in sub-humid and semi-arid climates.

Improving the accuracy of reference evapotranspiration (RET) estimation is essential for effective water resource management, irrigation planning, and climate change assessments in agricultural systems. The FAO-56 Penman-Monteith (PM-FAO56) model, a widely endorsed approach for RET estimation, often encounters limitations due to the lack of complete meteorological data. This study evaluates the performance of eight empirical models and four machine learning (ML) models, along with their hybrid counterparts, in estimating daily RET within the Gharb and Loukkos irrigated perimeters in Morocco.

A Giant Nevus Lipomatosus Cutaneous Superficialis Treated With Serial Surgical Excision: A Case Report.

Nevus lipomatosus cutaneous superficialis (NLCS) is a rare dermatological condition characterized by ectopic mature adipocytes in the dermis. The classic variety presents multiple clustered skin-colored nodules, while the solitary form is a single papule or nodule. We report the case of a 25-year-old female who presented with multiple cerebriform nodules coalescing into a large plaque over the left iliac region that developed over 17 months.

Distal infusion stomal enteroclysis: An effective technique to manage postoperative enterostomal output.

Background: High output enterostomy leads to malnutrition and fluid/electrolyte loss which may be challenging to manage despite dietary modification, anti-motility, anti-secretory drugs, and parenteral nutrition. Distal infusion stomal enteroclysis (DISE) is an alternative to restore nutritional deficit and replace parenteral nutrition in resource-limited settings where treatment cost and availability of trained nurses are limiting factors.

Objective: To assess the effectiveness and feasibility of DISE in managing postoperative enterostomal output.

Spatiotemporal regulation of anther's tapetum degeneration paved the way for a reversible male sterility system in cotton.

Male sterility is an important agronomical trait in self-pollinating plants for producing cost-effective F1 hybrids to harness the heterosis. Still, large-scale development and maintenance of male sterile lines and restoring fertility in F1 hybrids pose significant challenges in plant hybrid breeding. Cotton is a self-pollinating crop and exhibits strong hybrid vigor.

Phyto-safe in vitro regeneration and harnessing antimicrobial-resistant endophytes as bioinoculants for enhanced growth and secondary metabolites yield in Nilgirianthus ciliatus.

Nilgirianthus ciliatus, extensively exploited for its pharmacological properties, is now classified as vulnerable. In vitro micropropagation offers a sustainable approach for ecological conservation and rational utilization of this biodiversity resource. This study aimed to reduce endophytes during in vitro propagation and isolating antimicrobial-resistant endophytes from N.

NGS-Based Metagenomics Depicting Taxonomic and Functional Insights into North-Western Himalayan Hot Springs.

Unlabelled: Hot springs have tremendous significance due to their divulging physiochemical features. In the recent past, metagenomics has emerged as a unique methodology to explore microbiota as well as new biocatalysts possessing advantageous biochemical properties from hot springs. In the present study, metagenomics has been employed for microbial diversity exploration and identification of genes involved in various metabolic pathways among two hot springs, Manikaran and Tatapani, located in Himachal Pradesh, India.

Promoter of COR2-like gene is a stress inducible regulatory region in banana.

A promoter is a crucial component in driving the expression of a transgene of interest for biotechnological applications in crop improvement and thus characterization of varied regulatory regions is essential. Here, we identified the promoter of COR2-like (codeinone reductase-like) from banana and characterized its tissue specific and stress inducible nature. MusaCOR2-like of banana is closely related to COR2 and CHR (chalcone reductase) sequences from different plant species and contains signature sequences including a catalytic tetrad typical of proteins with aldo-keto reductase activity.

Physiological significance of the two isoforms of initiator tRNAs in .

Unlabelled: possesses four initiator tRNA (i-tRNA) genes, three of which are present together as and the fourth one as . In B, all four genes ( and ) encode i-tRNA, in which the G at position 46 is modified to mG46 by TrmB (mG methyltransferase). However, in K, because of a single-nucleotide polymorphism, encodes a variant, i-tRNA, having an A in place of mG46.

Mycobacterial biofilms: Understanding the genetic factors playing significant role in pathogenesis, resistance and diagnosis.

Even though the genus Mycobacterium is a diverse group consisting of a majority of environmental bacteria known as non-tuberculous mycobacteria (NTM), it also contains some of the deadliest pathogens (Mycobacterium tuberculosis) in history associated with chronic disease called tuberculosis (TB). Formation of biofilm is one of the unique strategies employed by mycobacteria to enhance their ability to survive in hostile conditions. Biofilm formation by Mycobacterium species is an emerging area of research with significant implications for understanding its pathogenesis and treatment of related infections, specifically TB.

Lamotrigine-mediated rescue of RsgA-deficient reveals another role of IF2 in ribosome biogenesis.

Unlabelled: RsgA (small ribosomal subunit, 30S, GTPase), a late-stage biogenesis factor, releases RbfA from 30S-RbfA complex. Δ (deleted for ) shows a slow growth phenotype and an increased accumulation of 17S rRNA (precursor of 16S rRNA) and the ribosomal subunits. Here, we show that the rescue of the Δ strain by multicopy (IF2) is enhanced by simultaneous overexpression of initiator tRNA (i-tRNA), suggesting a role of initiation complex formation in growth rescue.

Protocol for the Development and Analysis of the Oxford and Reading Cognitive Comorbidity, Frailty and Ageing Research Database-Electronic Patient Records (ORCHARD-EPR).

Background: Hospital electronic patient records (EPRs) offer the opportunity to exploit large-scale routinely acquired data at relatively low cost and without selection. EPRs provide considerably richer data, and in real-time, than retrospective administrative data sets in which clinical complexity is often poorly captured. With population ageing, a wide range of hospital specialties now manage older people with multimorbidity, frailty and associated poor outcomes.

Aerosol-PM2.5 Dynamics: In-situ and satellite observations under the influence of regional crop residue burning in post-monsoon over Delhi-NCR, India.

The increasing air pollution in the urban atmosphere is adversely impacts the environment, climate and human health. The alarming degradation of air quality, atmospheric conditions, economy and human life due to air pollution needs significant in-depth studies to ascertain causes, contributions and impacts for developing and implementing an effective policy to combat these issues. This work lies in its multifaceted approach towards comprehensive understanding and mitigating severe pollution episodes in Delhi and its surrounding areas.

Exploring peptidomes of by-products generated during production using WS4 for identification of novel bioactive peptides.

The considerable value of whey is evident from its significant potential applications and contributions to the functional food and nutraceutical market. The by-products were individually obtained during functional and novel soy cheese production using defined lactic acid bacterial strains of Sikkim Himalaya's traditional . Hydrolysis of substrate proteins by starter proteinases resulted in a comparable peptide content in whey and soy whey which was associated with antioxidant and ACE inhibition potential.

Exploring the influence of organ differentiation on biosynthesis and accumulation of camptothecin in var. .

Unlabelled: Genus has recently emerged as one of the promising sources of Camptothecin (CPT), an antitumour monoterpene indole alkaloid. It possesses CPT in its every part and has a relatively short life span. To determine whether differentiation plays any role in the synthesis and/or accumulation of CPT, the concentration of CPT was analyzed across various tissues of var.

A Comprehensive Review of Minimally Invasive Dermatosurgical Procedures.

Minimally invasive dermatosurgical procedures have revolutionized the field of dermatology, offering patients effective treatment options with reduced risks and downtime. This review provides a comprehensive overview of these procedures, beginning with their definition and historical context. We classify minimally invasive techniques, including both surgical and nonsurgical approaches, and explore their wide-ranging applications in cosmetic and therapeutic dermatology.