Identification of food preservative, stress relief compounds by GC-MS and HR-LC/Q-TOF/MS; evaluation of antioxidant activity of leaves methanolic extract (in vitro) and polyphenolic fraction (in vivo).

The present paper has been designed to evaluate phytochemical profile, in vitro free radical scavenging activity, cytotoxicity of methanolic extract and in vivo antioxidant activity of polyphenolic fraction of leaves. Methanolic extract of leaves (MEAIL) contained rich amount of phenols, flavonoids and saponins. The GC-MS analysis of extract revealed 13 compounds, whereas HR-LC/Q-TOF/MS showed 87, and all were coincided with functional groups identified by FTIR.