An ultra-sensitive, multiplexed, and cost-effective POCT system for the detection of co-infecting respiratory viruses, including SARS-CoV-2, Flu A, Flu B, and RSV, within 30 min.

The co-circulation of respiratory viruses, including SARS-CoV-2, Influenza A (Flu A), Influenza B (Flu B), and respiratory syncytial virus (RSV), poses a significant public health threat. Timely recognition of these viruses allows healthcare professionals to implement effective infection control measures, allocate medical resources properly, and prevent complications from incorrect treatments. Multiplex nucleic acid testing Point-of-care test (mNAT-POCT) circumvents issues of traditional tests, such as high demands on laboratory environments, personnel, and equipment, and limited target analyses, allowing its use in point-of-care settings.

Recent in vitro advances in the ocular antimicrobial agents against Acanthamoeba.

This review examines the advancements in antimicrobial drug discovery with in vitro assays for Acanthamoeba, highlighting the efficacy of current topical antimicrobial agents. In recent decades, the treatment and diagnosis of Acanthamoeba keratitis (AK) have presented clinical challenges. Clinicians often rely on clinical judgment, risk factors, and patient travel history to guide initial treatment decisions.

A Facebow-Free Technique for Mechanical Articulator Remounting Using Computed Tomography.

This technical note introduces a technique for remounting dental casts to a mechanical articulator using computed tomography (CT) without a facebow. We have developed a jig method that ensures precise remounting by aligning the virtual dental casts to the upper and lower jaw models reconstructed from the CT and transferring them to a virtual articulator based on anatomical landmarks. A custom-designed positioning jig and splint further enhance the accuracy of the process, preserving the spatial relationship between the midface and mandible.

Search for MeV-Scale Axionlike Particles and Dark Photons with PandaX-4T.

Axionlike particles (ALPs) and dark photons (DPs) are viable dark matter particle candidates. We have searched for possible ALP/DP signals in the PandaX-4T liquid xenon detector using 440  kg·yr of data. A binned likelihood fit is constructed to search for possible mono-energetic peaks induced by the absorption processes between ALPs/DPs and atomic electrons of xenon.

High identification and positive-negative discrimination but limited detailed grading accuracy of ChatGPT-4o in knee osteoarthritis radiographs.

Purpose: To explore the potential of ChatGPT-4o in analysing radiographic images of knee osteoarthritis (OA) and to assess its grading accuracy, feature identification and reliability, thereby helping surgeons to improve diagnostic accuracy and efficiency.

Methods: A total of 117 anterior‒posterior knee radiographs from patients (23.1% men, 76.

Detection of Analytes with the Outer Surface of Solid-State Nanochannels: From pm to μm.

ConspectusAccurately simulating or sensitively monitoring specific substances, such as ions, molecules, and proteins in the life process, is essential for gaining a fundamental comprehension of the underlying biological mechanism, which has been a trending topic for many years. Solid-state nanochannels, inspired by biological ion channels, have been developed for decades and have achieved significant success, representing the forefront of the interdisciplinary fields of bioanalytical chemistry and nanotechnology. Typically, solid-state nanochannels with a pore size of less than 100 nm are selected to construct nanochannel-based biosensors, which can be an excellent platform to analyze small analytes, such as ions and small molecules, in a restricted space and simulate the intricate process of ion transport in living organisms.

Prognostic potential of neutrophil-to-lymphocyte ratio for appendicular skeletal muscle mass reduction in males aged 70 and older.

Inflammation plays a pivotal role in the age-related decline of skeletal muscle mass, leading to sarcopenia in the elderly. The prevalence of sarcopenia notably increases among males aged ≥ 70. However, it remains unclear whether inflammatory indexes are associated with the reduction in skeletal muscle mass in the elderly population.

Correlation of noninvasive imaging of tumour-infiltrating lymphocytes with survival and BCG immunotherapy response in patients with bladder cancer: a multicentre cohort study.

Background: Tumour-infiltrating lymphocytes (TILs) are strongly correlated with the prognosis and immunotherapy response in bladder cancer. The TIL status is typically assessed through microscopy as part of tissue pathology. Here, the authors developed Rad-TIL model, a novel radiomics model, to predict TIL status in patients with bladder cancer.

Evaluation of the Efficacy of the Traditional Chinese Medicine Formulation Ru-Yi-Jin-Huang-Saan on Colles Fracture After Surgery: Protocol for a Randomized, Double-Blind, Placebo-Controlled Trial.

Background: Colles fracture, a common wrist injury, often requires surgical intervention. After surgery, patients may experience persistent pain and reduced wrist function, potentially resulting in long-term disability. In clinical practice, traditional Chinese medicine practitioners frequently use Ru-Yi-Jin-Huang-Saan (RYJHS) to treat such patients in Taiwan.

Association of Screen Content With Early Development Among Preschoolers in Shanghai: 7-Day Monitoring Study With Auto Intelligent Technology.

Background: It is unclear how exposure to different types of screen content is associated with early development among preschool children.

Objective: This study aims to precisely evaluate the screen exposure time across different content types and to explore the associations with the Ages and Stages Questionnaire, Third Edition (ASQ-3) score and 5 capacity domains in children aged 34.5-66 months.

Auricular Acupressure Versus an Intermittent Low-Carbohydrate Diet in Children With Overweight or Obesity With Gastric-Heat and Dampness-Obstruction Syndrome: Protocol for a Randomized Controlled Trial.

Background: Childhood obesity frequently persists into adulthood and is associated with an increased risk and earlier onset of cardiovascular disease in later life. Behavioral change strategies have been proposed as the first-line weight management approach for children and adolescents with obesity. Nonpharmacological interventions, such as traditional Chinese medicine (TCM) auricular acupressure treatment and intermittent low-carbohydrate diet (ILCD), are increasingly being investigated in the young obese population.

Use of Artificial Intelligence, Internet of Things, and Edge Intelligence in Long-Term Care for Older People: Comprehensive Analysis Through Bibliometric, Google Trends, and Content Analysis.

Background: The global aging population poses critical challenges for long-term care (LTC), including workforce shortages, escalating health care costs, and increasing demand for high-quality care. Integrating artificial intelligence (AI), the Internet of Things (IoT), and edge intelligence (EI) offers transformative potential to enhance care quality, improve safety, and streamline operations. However, existing research lacks a comprehensive analysis that synthesizes academic trends, public interest, and deeper insights regarding these technologies.

Radiation Dose-Volume Effects on Negative Tumor-Draining Lymph Nodes Impacted T-Cell Activation and Prognosis in Esophageal Cancer with Chemoradiotherapy.

Purpose: Preclinical model found that elective nodal irradiation (ENI) attenuated the efficacy of radiotherapy and radio-immunotherapy. However, limited clinical studies have explored the correlation between radiation dose-volume parameters of negative tumor draining lymph nodes (TDLNs) and T-cell activation/prognosis for cancer patients treated with definitive radio(chemo)therapy.

Experimental Design: Patients with locally advanced esophageal cancer undergoing definitive chemoradiotherapy (CRT) were selected from two prospective trials.

Multi-Axis Feature Diversity Enhancement for Remote Sensing Video Super-Resolution.

How to aggregate spatial-temporal information plays an essential role in video super-resolution (VSR) tasks. Despite the remarkable success, existing methods adopt static convolution to encode spatial-temporal information, which lacks flexibility in aggregating information in large-scale remote sensing scenes, as they often contain heterogeneous features (e.g.

Citrulline regulates macrophage metabolism and inflammation to counter aging in mice.

Metabolic dysregulation and altered metabolite concentrations are widely recognized as key characteristics of aging. Comprehensive exploration of endogenous metabolites that drive aging remains insufficient. Here, we conducted an untargeted metabolomics analysis of aging mice, revealing citrulline as a consistently down-regulated metabolite associated with aging.

Rewilding catalyzes maturation of the humoral immune system.

Inbred mice used for biomedical research display an underdeveloped immune system compared with adult humans, which is attributed in part to the artificial laboratory environment. Despite representing a central component of adaptive immunity, the impact of the laboratory environment on the B cell compartment has not been investigated in detail. Here, we performed an in-depth examination of B cells following rewilding, the controlled release of inbred laboratory mice into an outdoor enclosure.

Penalised regression improves imputation of cell-type specific expression using RNA-seq data from mixed cell populations compared to domain-specific methods.

Gene expression studies often use bulk RNA sequencing of mixed cell populations because single cell or sorted cell sequencing may be prohibitively expensive. However, mixed cell studies may miss expression patterns that are restricted to specific cell populations. Computational deconvolution can be used to estimate cell fractions from bulk expression data and infer average cell-type expression in a set of samples (e.

Patient-Selection of a Clinical Trial Primary Outcome: The ENHANCE-AF Outcomes Survey.

Introduction: Before the initiation of the ENHANCE-AF clinical trial, which tested a novel digital shared decision-making tool to guide the use of anticoagulants in stroke prevention for patients with atrial fibrillation, this study aimed to identify the most appropriate, patient-selected primary outcome and to examine whether outcome selection varied by demographic and clinical characteristics.

Methods: Our cross-sectional survey asked 100 participants with atrial fibrillation to rank two alternative scales based on the scales' ability to reflect their experiences with decision-making for anticoagulation. The Decisional Conflict Scale (DCS), a 16-item scale, measures perceptions of uncertainty in choosing options.

A Sterically Tuned 2-Fluoropyridinium Salt for the Catalyst-Free, Visible-Light-Mediated Deoxygenation of Alcohols via an Electron Donor-Acceptor Complex.

We here report the use of a sterically tuned 2-fluoro-1-methylpyridinium salt for the catalyst-free, visible-light-mediated deoxygenative transformation of alcohols through alkoxypyridinium intermediates. The key to this process is the introduction of a bulky cyclohexyl group at the 3-position of the pyridinium ring, which enforces a favorable conformation for C-O bond cleavage and the formation of an electron donor-acceptor complex between the pyridinium ring and amines.

Combining Apatinib and Oxaliplatin Remodels the Immunosuppressive Tumor Microenvironment and Sensitizes Desert-Type Gastric Cancer to Immunotherapy.

Immune checkpoint blockade (ICB) therapies have achieved significant breakthroughs in cancer treatment over the past decade. However, ICB is largely ineffective in desert-type gastric cancer (GC) due to intrinsic tumor heterogeneity and a highly immunosuppressive tumor microenvironment (TME). Transforming tumors from immunosuppressive to immunostimulatory is a potential approach to enhance ICB response.

Brain White Matter Changes in Non-demented Individuals with Color Discrimination Deficits and Their Association with Cognitive Impairment: A NODDI Study.

Previous studies have found associations between color discrimination deficits and cognitive impairments besides aging. However, investigations into the microstructural pathology of brain white matter (WM) associated with these deficits remain limited. This study aimed to examine the microstructural characteristics of WM in the non-demented population with abnormal color discrimination, utilizing Neurite Orientation Dispersion and Density Imaging (NODDI), and to explore their correlations with cognitive functions and cognition-related plasma biomarkers.

Potential mechanism of impaired perceptual reasoning in children with obstructive sleep apnea syndrome: topological analysis of brain white matter network employing graph theory.

Childhood obstructive sleep apnea syndrome (OSAS) disrupts normal ventilation and sleep structure and affects cognitive functions. However, the neurophysiological mechanisms underlying cognitive impairment are unclear. This study investigates the topological connectivity of white matter networks in children with moderate to severe OSAS and explores the underlying mechanisms of cognitive impairment.

Degradation and detoxification of aflatoxin B1 by two peroxidase enzymes from Irpex lacteus F17.

Aflatoxin B1 (AFB1), the most toxic mycotoxin produced by some Aspergillus species, is commonly found in agricultural products, especially grains, and poultry feeds. Enzymic degradation is considered to be the most promising detoxification method, because it is efficient, safe and causes minimal damage to the nutritional quality of treated foods. In this study, a recombinant manganese peroxidase (Il-MnP1) and a recombinant dye-decolorizing peroxidase (Il-DyP4) from Irpex lacteus F17 were used to degrade AFB1, either individually or in combination.

Exploring seed-based upcycled oils: types, extraction processes, and emerging applications.

The agri-food sector generates substantial by-products, particularly from fruits and vegetables, which often contribute to food waste. Among these by-products, seeds are frequently overlooked despite their high potential for valuable applications. Recognizing this unexplored potential, this literature review categorizes and describes various seed-based upcycled oils, highlighting their potential for commercial use and their nutritional benefits.