Immobilization of Urokinase onto Magnetically Directed Micromotors.

In this presented work, a new micromotor was prepared for urokinase immobilization. A covalent bond was constructed between the urokinase and the carboxyl groups of the graphene oxide, which is located at the outer layer of micromotors by EDC/NHS chemistry. The inner nickel layer gave magnetic properties to the micromotors and enables them to be separated from the reaction medium with the help of a simple magnet.

Boron nitride nanosheet modified label-free electrochemical immunosensor for cancer antigen 125 detection.

In this presented study, a new boron nitride nanosheets modified label-free electrochemical immunosensors were prepared for early detection of cancer antigen 125 (CA125). To aim for, boron nitride (BN) nanosheets were synthesized by conventional sonication-assisted method and then characterized. BN nanosheets were used for the surface modification of the working electrode of the screen-printed electrode (SPE).

Inulinase Immobilized Lectin Affinity Magnetic Nanoparticles for Inulin Hydrolysis.

In this presented paper, concanavalin A-modified cysteine-functionalized FeO/Ag core/shell magnetic nanoparticles were synthesized and used as a support material for inulinase enzyme, which has been intensively used for the preparation of high-fructose syrup by hydrolyzing inulin. Inulinase adsorption capacity of Con A-functionalized Ag-coated magnetic nanoparticles was optimized by changing medium pH, temperature, and initial inulinase concentration, and maximum inulinase adsorption capacity was found to be 655.32 mg/g nanoparticle by using 1.

Peroxidase Immobilized Cryogels for Phenolic Compounds Removal.

In this presented work, preparation of poly(AAm) cryogel, peroxidase immobilization onto the poly(AAm) cryogel, and usability of these enzyme modified cryogels for phenolic compounds removal were described. For this purpose, poly(AAm) cryogels were synthesized by using cryocopolymerization technique at sub-zero temperatures, and covalently functionalized with peroxidase enzyme by EDC/NHS chemistry. Characterization of the cryogels was carried out by FTIR, SEM, and EDX analysis.

Bacteria killer enzyme attached magnetic nanoparticles.

The objective of the present work was to develop immobilized lysozyme systems through adsorption on magnetic nanoparticles for potential usage in bacteria killing studies. For this, magnetic poly(HEMA-GMA) nanoparticles were prepared by surfactant free emulsion polymerization technique and functionalized with dye ligand Reactive Green 5. Synthesized magnetic nanoparticles were then characterized by FTIR, SEM, EDX and ESR studies.

Enzymatic Activity of Urokinase Immobilized onto Cu-Chelated Cibacron Blue F3GA-Derived Poly (HEMA) Magnetic Nanoparticles.

In this presented work, magnetic poly(2-hydroxyethyl methacrylate) (p (HEMA)) nanoparticles were synthesized by surfactant-free emulsion polymerization technique. Cibacron Blue F3GA was covalently attached to the magnetic p (HEMA) nanoparticles and Cu ions were then chelated with dye molecules. Synthesized magnetic nanoparticles were spherical with the diameter of 80 nm and exhibited magnetic character.