Wohlfahrtiimonas chitiniclastica Bacteremia: A Rare Case of a Male With Maggot-Infested Lower Extremity Wounds.

 () is an emerging gram-negative bacillus rarely found in patients presenting with fly myiasis or parasitic larvae infection. Here, we present the case of a 58-year-old male who presented with bacteremia from lower extremity wounds complicated by fly larvae infestation. Blood cultures were analyzed with matrix-assisted laser desorption ionization-time of flight mass spectrometry, which identified .

Circulating tumor cell investigation in breast cancer patient-derived xenograft models by automated immunofluorescence staining, image acquisition, and single cell retrieval and analysis.

Background: Breast cancer patient-derived xenograft (BC-PDX) models represent a continuous and reproducible source of circulating tumor cells (CTCs) for studying their role in tumor biology and metastasis. We have previously shown the utility of BC-PDX models in the study of CTCs by immunohistochemistry (IHC) on serial paraffin sections and manual microscopic identification of cytokeratin-positive cells, a method that is both low-throughput and labor-intensive. We therefore aimed to identify and characterize CTCs from small volume mouse blood samples and examined its practical workflow in a study of BC-PDX mice treated with chemotherapy using an automated imaging platform, the AccuCyte®-CyteFinder® system.