Engineered extracellular vesicles carrying let-7a-5p for alleviating inflammation in acute lung injury.

Background: Acute lung injury (ALI) is a life-threatening respiratory condition characterized by severe inflammation and lung tissue damage, frequently causing rapid respiratory failure and long-term complications. The microRNA let-7a-5p is involved in the progression of lung injury, inflammation, and fibrosis by regulating immune cell activation and cytokine production. This study aims to use an innovative cellular electroporation platform to generate extracellular vesicles (EVs) carring let-7a-5p (EV-let-7a-5p) derived from transfected Wharton's jelly-mesenchymal stem cells (WJ-MSCs) as a potential gene therapy for ALI.

Maternal hypercholesterolemia exacerbates atherosclerosis lesions in female offspring through potentiating macrophage polarization toward an inflammatory M1 phenotype.

Maternal hypercholesterolemia induces early onset of cardiovascular diseases in offspring; however, its underlying mechanism remains poorly understood. We hypothesized that maternal hypercholesterolemia increases offspring susceptibility to atherosclerosis in adulthood through developmental modifications of macrophages. Female apolipoprotein E (ApoE)-deficient mice were fed a Western-type diet (WD) or a control diet (CD) prior to and throughout gestation and lactation.

Substituent effects of AIE-active α-cyanostilbene-containing triphenylamine derivatives on electrofluorochromic behavior.

Four aggregation-induced emission (AIE)- and electro-active cyanostilbene-based triphenylamine-containing derivatives with different substituents were synthesized to investigate their effects on the photoluminescence properties and electrochromic (EC) and electrofluorochromic (EFC) behavior of gel-type electrochromic devices (ECDs). The optical and photoluminescence properties of the obtained materials were influenced by the substituents, and revealed AIE-active characteristics, exhibiting stronger fluorescence intensity in the aggregated state than in solution. Consequently, the EFC devices could be fabricated by combining these AIE- and electro-active materials with cathodic EC heptyl viologen HV into the gel-type electrolyte system to enhance the emission intensity, on/off contrast ratio, and response capability.

Biofilm formation of beta-hemolytic group G Streptococcus dysgalactiae subspecies equisimilis isolates and its association with emm polymorphism.

Biofilm formation has been well known as a determinant of bacterial virulence. Group G Streptococcus dysgalactiae subspecies equisimilis (SDSE), a relevant pathogen with increasing medical importance, was evaluated for the biofilm-forming potential. Microtiter plate assay was used to assess the most feasible medium for group G SDSE to form a biofilm.