A simple, cost-efficient stability-indicating RP-HPLC method for simultaneous estimation of embelin and piperine for routine analysis of marketed polyherbal capsules and tablets.

Embelin (EMB) and Piperine (PIP) alkaloids are reported for -antidiabetic, hypolipidemic, antioxidant, and anti-cancer properties. However, simultaneous analytical methods are scarce. A stability-indicating RP-HPLC method was developed with mobile phase MeOH: 0.

Anti-oxidants as therapeutic agents for oxidative stress associated pathologies: future challenges and opportunities.

Free radicals have been implicated in the pathogenesis of cancer along with cardiovascular, neurodegenerative, pulmonary and inflammatory disorders. Further, the relationship between oxidative stress and disease is distinctively established. Clinical trials using anti-oxidants for the prevention of disease progression have indicated some beneficial effects.

Rapid detection of the invasive tomato leaf miner, Phthorimaea absoluta using simple template LAMP assay.

The tomato leaf miner (TLM), Phthorimaea absoluta Meyrick, 1917 (Lepidoptera: Gelechiidae) is a destructive invasive insect that has expanded its global distribution. Rapid and accurate identification of invasive pests is essential to support subsequent management and devise control measures. To accurately diagnose P.

Synthesis, X-ray, antioxidant, in-vitro biological & in-silico docking studies of novel organoselenides: Promising colorectal cancer inhibitors.

A series of multi-target organoselenides 3a-h has been synthesized with the advantages of a simple operation, and good yields of 66-89 % escorted by mechanistic enlightenment. The compounds 3b, 3c continued to exist as orthorhombic and trigonal, whereas 3d exist as monoclinic confirmed by the X-ray crystallography. Organoselenides 3c and 3f displayed the highest % radical scavenging potential with % inhibition of 98.

Pterygium extended removal followed by minor ipsilateral simple limbal epithelial transplantation (PERMISLET): An innovative technique for recurrent pterygium.

We describe a novel technique for recurrent pterygium and assess the advantage of properties of extended tenonectomy, amniotic membrane transplantation, and limbal epithelial transplantation in terms of recurrence rate, postoperative symptoms, postoperative orthoptics, and other complications. A total of nine eyes with recurrent pterygium underwent PERMISLET, i.e.

The eSAH score: a simple practical predictive model for SAH mortality and outcomes.

We developed a simple quantifiable scoring system that predicts aneurysmal subarachnoid hemorrhage (aSAH) mortality, delayed cerebral ischemia (DCI), and modified Rankin scale (mRS) outcomes using readily available SAH admission data with SAH volume (SAHV) measured on computed tomography (CT). We retrospectively analyzed a cohort of 277 patients with aSAH admitted at our Comprehensive Stroke Center at Mayo Clinic in Jacksonville, Florida, between January 5, 2012, and February 24, 2022. We developed a mathematical radiographic model SAHV that measures basal cisternal SAH blood volume using a derivation of the ABC/2 ellipsoid formula (A = width/thickness, B = length, C = vertical extension) on noncontrast CT, which we previously demonstrated is comparable to pixel-based manual segmentation on noncontrast CT.

DEEP LEARNING-BASED FRAMEWORK TO DETERMINE THE DEGREE OF COVID-19 INFECTIONS FROM CHEST X-RAY.

The corona virus disease-19 (COVID-19) epidemic, the whole globe is suffering from a medical condition catastrophe that is unprecedented in scale. As the coronavirus spreads, scientists are worried about offering or helping in the supply of remedies to preserve lives and end the epidemic. Artificial intelligence (AI), for example, has occurred altered to deal with the difficulties raised by pandemics.

Synthesis and Characterization of N-Doped Carbon Quantum Dots and its Application for Efficient Delivery of Curcumin in Live Cell.

To improve bioavailability, enhance the solubility and stability of the hydrophobic drug curcumin, nanoparticles such as carbon quantum dots (CQDs) are unique choices. In this study, we present a simple, cost-effective, and eco-friendly method for synthesizing nitrogen-doped carbon quantum dots (N-CQDs) and their application in the efficient delivery of hydrophobic drugs curcumin into live cancer cells. The N-CQDs produced in this study exhibit excellent water solubility, remarkable stability, and high biocompatibility.

International Validation of a Novel PEACE Scale to Improve the Quality of Upper Gastrointestinal Mucosal Inspection During Endoscopy.

Introduction: The performance of a high quality esophagogastroduodenoscopy (EGD) is dependent on the mucosal cleanliness. Recently, the Polprep: Effective Assessment of Cleanliness in EGD (PEACE) scale was created to assess the degree of mucosal cleanliness during EGD. The aim of this study was to validate this scoring system in a cohort of international endoscopists.

Staff education compared with active real-time waste segregation to reduce the environmental impact of hip and knee arthroplasty.

Aims: Arthroplasty has been shown to generate the most waste among all orthopaedic subspecialties, and it is estimated that hip and knee arthroplasty generate in excess of three million kg of waste annually in the UK. Infectious waste generates up to ten times more CO2 compared with recycled waste, and previous studies have shown that over 90% of waste in the infectious stream is misallocated. We assessed the effect of real-time waste segregation by an unscrubbed team member on waste generation in knee and hip arthroplasty cases, and compared this with a simple educational intervention during the 'team brief' at the start of the operating list across two sites.

A simple freeze-thaw based method for efficient purification of recombinant human proinsulin from inclusion bodies.

Insulin is a pivotal peptide hormone essential for regulating glucose homeostasis. It has been known for over 100 years, but its production and purification methods are still under improvement. Escherichia coli based bacterial expression system is primarily used for insulin production.

Nanostructured Boron-Doped Ultra-Nanocrystalline Diamond Micro-Pyramids: Efficient Electrochemical Supercapacitors.

The miniaturization of electrochemical supercapacitors (EC-SCs) requires electrode materials that are both durable and efficient. Boron-doped diamond (BDD) films are an ideal choice for EC-SC due to their durability and exceptional electrochemical performance. In this study, nanostructured boron-doped ultra-nanocrystalline diamonds (NBUNCD) are fabricated on Si micro-pyramids (Si) using a simple reactive ion etching (RIE) process.

Development of validated HPTLC methods for determination of triterpenoids and flavan-3-ols in leaf and stembark of Dubard.

Dubard is a highly reputed medicinal plant of Ayurveda. In the current study, High-performance Thin-layer chromatographic (HPTLC) techniques were established for the analysis of triterpenoids like lupeol (LU), betulinic acid (BA), epimeric flavan-3-ols (+)-catechin (CA), and (-)-epicatechin (ECA), in its leaf and stembark. HPTLC separation was performed using petroleum ether: ethyl acetate: toluene: formic acid (7:2:1:0.

Water-mediated ribonucleotide-amino acid pairs and higher-order structures at the RNA-protein interface: analysis of the crystal structure database and a topological classification.

Water is essential for the formation, stability and function of RNA-protein complexes. To delineate the structural role of water molecules in shaping the interactions between RNA and proteins, we comprehensively analyzed a dataset of 329 crystal structures of these complexes to identify water-mediated hydrogen-bonded contacts at RNA-protein interface. Our survey identified a total of 4963 water bridges.

Faeco-vaginal fistulae: An algorithm for management.

Faeco-vaginal fistulae are not uncommon. We suggest a simple management algorithm to provide tailored approach for every patient.

Integrating traditional QSAR and read-across-based regression models for predicting potential anti-leishmanial azole compounds.

Leishmaniasis, a neglected tropical disease caused by various Leishmania species, poses a significant global health challenge, especially in resource-limited regions. Visceral Leishmaniasis (VL) stands out among its severe manifestations, and current drug therapies have limitations, necessitating the exploration of new, cost-effective treatments. This study utilized a comprehensive computational workflow, integrating traditional 2D-QSAR, q-RASAR, and molecular docking to identify novel anti-leishmanial compounds, with a focus on Glycyl-tRNA Synthetase (LdGlyRS) as a promising drug target.

Optimized and Simplified Technique for the Production and Culture of Precision-Cut Liver Slices.

This protocol presents a simple system for the creation and culture of Precision-cut Liver Slices (PCLS). PCLS contains all cells in an intact environment and, therefore, resembles a mini model of the whole organ. They enable the study of live tissues while replicating their complex phenotypes.

Steri-Strip Sling Technique - A Simple Procedure for the Management of Ingrown Toenails - A Prospective Study.

Background: Ingrown toenail is a nail disorder that commonly affects great toenails. Surgical modalities are commonly used as first-line treatment. A conservative approach is preferable as surgical procedures lead to pain and affect routine activities.

Assessment of genetic diversity and population structure analysis in Himalayan garlic genotypes from Sikkim using SSR markers.

Background: Garlic (Allium sativum L.) is an important crop with significant medicinal and culinary uses. Understanding the genetic diversity and population structure of Himalayan garlic genotypes from Sikkim is crucial for effective breeding and conservation efforts.

A novel dual-staining method for cost-effective visualization and differentiation of microbial biofilms.

Microbial biofilms are intricate communities that pose significant challenges in clinical and microbiological settings due to their resistance to antibiotics and immune responses. Advanced microscopy techniques, such as scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and fluorescence microscopy, are often employed to visualize and differentiate between these biofilms. However, these methods are not feasible in all laboratories because of their high cost and complexity.

Cardiac Papillary Fibroelastoma and Cerebrovascular Events: A Systematic Review.

Background: Cardiac papillary fibroelastomas (CPFs) are benign endocardial tumours with embolic potential. This article is a systematic review to identify the clinical profile, diagnosis, tumour characteristics, and treatment modalities in patients with CPF presenting with stroke or transient ischemic attack (TIA).

Methods: This systematic review was conducted using the PubMed and Embase databases, including case reports and/or series and observational studies (for a search period of up until April 2022).