Modern photonic devices demand low-cost, scalable methods for creating periodic patterns over diverse surfaces including nonplanar and tipped ones, the examples of which can be readily found in fiber optics. Laser-induced periodic surface structures (LIPSS) offer an attractive route for fabricating such patterns in a single-step straightforward procedure, where the temporal and spatial locality of the self-interference effects ensure robustness against variations of the laser processing parameters. In this work, we show the LIPSS-assisted oxidation of thin titanium films by near-IR femtosecond laser pulses as a promising technology for the production of regular gratings consisting of rutile ridges.
View Article and Find Full Text PDFMultimode fibers are attractive for high-power lasers if transverse modes are efficiently controlled. Here, a dielectric thin-film mirror (R~20%) is micro-fabricated on the central area of the end face of a 1 km multimode 100/140 µm graded-index fiber and tested as the output mirror of a Raman laser with highly multimode (M~34) 940 nm diode pumping. In the cavity with highly reflective input FBG, Raman lasing of the Stokes wave at 976 nm starts at the threshold pump power of ~80 W.
View Article and Find Full Text PDFGeorgian Med News
April 2024
HIV infection is one of the most acute problems of our time, characterized by slow development, prolonged course, and numerous clinical manifestations. Currently, there is a large number of drugs acting on different processes of human immunodeficiency virus replication, which constitute the group of highly active antiretroviral therapy (HAART). This article shows a theoretical review of modern HAART and analyzes the prescribed treatment regimens for patients with HIV infection.
View Article and Find Full Text PDFA recombinant humanized monoclonal antibody (mAb) Eculizumab, C5-complement cascade inhibitor, is an important treatment of complement-based diseases recommended by international guidelines. Elizaria® Drug Product (DP), developed by IBC Generium, Russia, is the world's first registered biosimilar of eculizumab (Soliris®, Alexion Pharmaceuticals). Using sensitive state-of-the-art analytical techniques extensive similarity assessment has been conducted to demonstrate the structural and functional similarity of original Soliris® (Eculizumab Reference Product, RP) and the biosimilar Elizaria®, focusing on the physicochemical and biological quality attributes, including those known to affect the mechanisms of action.
View Article and Find Full Text PDFPharmaceuticals (Basel)
November 2021
The disfunction or deficiency of the C1 esterase inhibitor (C1INH) is associated with hereditary or acquired angioedema (HAE/AAE), a rare life-threatening condition characterized by swelling in the skin, respiratory and gastrointestinal tracts. The current treatment options may carry the risks of either viral infection (plasma-derived Berinert) or immune reaction (human recombinant C1INH from rabbit milk, Ruconest). This study describes the physicochemical and biological characterization of a novel recombinant human C1 esterase inhibitor (rhC1INH) from Chinese hamster ovary (CHO) cells for the treatment of hereditary angioedema compared to the marketed products Berinert and Ruconest.
View Article and Find Full Text PDFAntibodies specific to β-Glucocerebrosidase were selected from phage displayed naïve scFv libraries. Biopannings were performed against recombinant human protein β-Glucocerebrosidase immobilized on polystyrene surface, specific phages were eluted with 50% ethylene glycol in citrate buffer (pH 6.0).
View Article and Find Full Text PDFTherapeutic monoclonal antibodies (mAbs) are within the fastest growing group of pharmaceuticals on the global market. IgG1 subclass is the most potent effector in Fc-related functions. The N-linked glycosylation of mAbs Fc-domain significantly influences its therapeutic activity and the presence of this modification is largely dependent on producer cell and parameters of manufacturing process.
View Article and Find Full Text PDFA new variant of all-fiber multiple-beam interferometer capable to perform narrow-band filtration of a reflected light, with characteristics similar to those for Fabry-Perot interferometer in a transmission, is presented. The interferometer design accompanied with parameters simulation is conducted, the experimental sample is fabricated and the study of its characteristics is undertaken. Experimental results conform the calculations.
View Article and Find Full Text PDFZh Nevrol Psikhiatr Im S S Korsakova
July 2014
The results of psychological examination of 33 male patients with a diagnosis of schizotypal disorder (SD), mean age 22 years, with age at onset in adolescence and 20 healthy young men are presented. Cognitive performance in patients with SD did not differ from healthy people on all measures, with the exception of social adaptation. There was a trend towards the change of normative responses and, respectively, estimation of the results of psychological techniques.
View Article and Find Full Text PDFThe effects of nanodispersed iron forms on the morphology and function of the blood system were studied. Maghemite and lepidocrocite caused a leukocytic shift towards segmented neutrophil forms, reduction of lymphocyte rigidity, and stimulated their compactization. In addition, the counts of small hyperchromatic erythrocytes with high rigidity increased in the blood flow.
View Article and Find Full Text PDFFlowering plants in Australia have been geographically isolated for more than 34 million years. In the Northern Hemisphere, previous work has revealed a close fit between the optimal discrimination capabilities of hymenopteran pollinators and the flower colours that have most frequently evolved. We collected spectral data from 111 Australian native flowers and tested signal appearance considering the colour discrimination capabilities of potentially important pollinators.
View Article and Find Full Text PDFThe interaction between flowers and insect pollinators is an important aspect of the reproductive mechanisms of many plant species. Several laboratory and field studies indicate that raising flower temperature above ambient can be an advantage in attracting pollinators. Here we demonstrate that this preference for warmer flowers is, in fact, context-dependent.
View Article and Find Full Text PDFThe possibility of using the atomic-force microscopy for the evaluation of the geometrical characteristics of blood cells has been shown. It has been found by comparing the hemocyte morphometric indices obtained at different modes of scanning that noncontact and semicontact methods are adequate for the estimation of the size and geometry of biological objects. The scanning of cells in the semicontact mode leads to the irreversible deformation of samples.
View Article and Find Full Text PDFThe polymerase complexes of double-stranded RNA (dsRNA) viruses are multifunctional RNA processing machineries that carry out viral genome packaging, replication, and transcription. The polymerase complex forms the innermost virion shell and is structurally related in dsRNA viruses infecting a diversity of host organisms. In this study, we analyzed the properties and functions of the minor polymerase complex protein P7 of dsRNA bacteriophage phi6 using terminally truncated P7 polypeptides and an in vitro self-assembly system established for the phi6 polymerase complex.
View Article and Find Full Text PDFActa Crystallogr D Biol Crystallogr
December 2004
Bacteriophage varphi12 protein P7 is a structural component of the polymerase complex and ensures stable packaging of the genomic RNA. varphi12 P7 has been cloned, purified and crystallized. Crystals belong to space group P3(2)21, with unit-cell parameters a = 75.
View Article and Find Full Text PDFUrol Nefrol (Mosk)
November 1994
Basing on the data obtained on percutaneous removal of stag-horn nephroliths in 81 patients (44 females and 37 males aged 10-65), the authors discuss the operative technique and surgical results. The patients were divided into 4 groups depending on the stone size, its position in the pelvicaliceal system, the presence and severity of calicectasis. Nephroliths sizes ranged from 3.
View Article and Find Full Text PDFUsing several HIV-1-specific antibodies and the immunogold labelling technique, we have detected and localized distinct viral proteins on ultrathin sections of HIV-1 infected cells embedded in the Lowicryl K4M resin. Monoclonal antibodies (MAbs) against p24, p17 and gp160/gp41 showed a preferential labelling on viral formations still attached to the cell membrane (budding process) or free in the extracellular space. The anti-p24 and the anti-p17 MAb yielded a gold labelling not only on mature but also on immature virions where the gold particles were associated with the ring-like electron-dense material.
View Article and Find Full Text PDFThe paper presents the results of telelithotripsy monotherapy for 34 staghorn stones in 32 nephrolithiasis children carried out at the RSFSR Research Institute of Urology, Moscow. Commonest manifestations of the disease were leukocyturia (87.5% of cases) and Proteus bacteriuria (31.
View Article and Find Full Text PDFThe paper describes the enzyme immunoassay system for detection of human immunodeficiency virus antigens, which is based on the use of rabbit anti-HIV antibodies and monoclonal antibodies to HIV-1 gene proteins gag. The system may be useful in the examination of laboratory and clinical samples to reveal both free and conjugated antigens in the composition of immune complexes. The sensitivity of the assay system under development is 0.
View Article and Find Full Text PDFThe authors describe results of transurethral endoscopic treatment of ureterocele in 61 patients, two techniques of a relevant operative intervention, indications, complications of an early (pyelonephritis) and late (vesicoureteral reflux) postoperative period, measures of their prevention. Advancing impairment of the urine outflow via the upper urinary tracts on ureterocele side necessitates earlier surgery (transurethral dissection or resection of ureterocele). Good 4-year follow-up results (normal function of the kidney, absence of pyelonephritis attacks and vesicoureteral reflux) suggest endoscopic transurethral treatment of ureterocele to be a method of choice the failure of which implies open surgical intervention.
View Article and Find Full Text PDFThe Urat-P lithotriptor made in this country was employed for remote lithotripsy (RLT) in 94 children with urolithiasis. Altogether 132 sessions were conducted. In 120 cases the concrement disintegration progressed in a sparing regimen (impulse duration 0.
View Article and Find Full Text PDFA competitive time-resolved fluoroimmunoassay (TR-FIA) system for the detection of antibodies to protein p24 of HIV was developed on the basis of monoclonal antibodies. The advantages of this test system over analogous enzyme immunoassay system and commercial test system "Antigen" (USSR) were demonstrated. The newly developed test system of TR-FIA was used for examination of sera from HIV-infected persons.
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