Publications by authors named "Simone Dill"
Article Synopsis
- Bispecific antibodies (bsAbs) were developed to deliver small interfering RNA (siRNA) specifically to cancer cells by targeting tumor antigens while also binding to digoxigenin (Dig).
- The bsAb-siRNA complexes effectively internalized into cells, but the release of siRNA into the cytoplasm was initially insufficient for mRNA knockdown.
- To enhance delivery and achieve effective mRNA knockdown, the siRNA was formulated into nanoparticles, enabling targeted siRNA delivery in a variety of cell types and showing promising results in tumor models in mice.
In this study, we explored the application of a yeast three-hybrid (Y3H)-based compound/protein display system to scanning the proteome for targets of kinase inhibitors. Various known cyclin-dependent kinase (CDK) inhibitors, including purine and indenopyrazole analogs, were displayed in the form of methotrexate-based hybrid ligands and deployed in cDNA library or yeast cell array-based screening formats. For all inhibitors, known cell cycle CDKs as well as novel candidate CDK-like and/or CDK-unrelated kinase targets could be identified, many of which were independently confirmed using secondary enzyme assays and affinity chromatography.
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