Publications by authors named "Simon R Bungers"
Article Synopsis
- Presynaptic active zones (AZs) are specialized structures in neurons that manage synaptic vesicle interactions essential for neurotransmitter release.
- Different forms of Munc13 proteins, particularly Munc13-1 and bMunc13-2, play crucial roles in regulating short-term plasticity in synapses, affecting neuronal function.
- The recruitment of these Munc13 proteins to synapses is regulated by specific interactions with AZ proteins like RIMs and ELKS1, highlighting the complexity and functional diversity of synaptic connections.
For decades, neuroscientists have used enriched preparations of synaptic particles called synaptosomes to study synapse function. However, the interpretation of corresponding data is problematic as synaptosome preparations contain multiple types of synapses and non-synaptic neuronal and glial contaminants. We established a novel Fluorescence Activated Synaptosome Sorting (FASS) method that substantially improves conventional synaptosome enrichment protocols and enables high-resolution biochemical analyses of specific synapse subpopulations.
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