Design and in vitro anticancer assessment of a click chemistry-derived dinuclear copper artificial metallo-nuclease.

Copper compounds with artificial metallo-nuclease (AMN) activity are mechanistically unique compared to established metallodrugs. Here, we describe the development of a new dinuclear copper AMN, Cu2-BPL-C6 (BPL-C6 = bis-1,10-phenanthroline-carbon-6), prepared using click chemistry that demonstrates site-specific DNA recognition with low micromolar cleavage activity. The BPL-C6 ligand was designed to force two redox-active copper centres-central for enhancing AMN activity-to bind DNA, via two phenanthroline ligands separated by an aliphatic linker.

Analysis of non-canonical three- and four-way DNA junctions.

The development of compounds that can selectively bind with non-canonical DNA structures has expanded in recent years. Junction DNA, including three-way junctions (3WJs) and four-way Holliday junctions (HJs), offer an intriguing target for developmental therapeutics as both 3WJs and HJs are involved in DNA replication and repair processes. However, there are a limited number of assays available for the analysis of junction DNA binding.

Antitumor copper(II) complexes with hydroxyanthraquinones and N,N-heterocyclic ligands.

Five ternary copper(II) complexes, [Cu(phen)(L1)(ClO)] (1), [Cu(phen)(L1)(DMSO)](PF6) (2), [Cu(bpy)(L1)(ClO)(HO)] (3), [Cu(dmp)(L1)(ClO)(HO)] (4), and [Cu(phen)(L2)](ClO) (5), in which phen = 1,10-phenanthroline, bpy = 2,2'-bipyridine, dmp = 2,9-dimethyl-1,10-phenanthroline, HL1 = 1,4-dihydroxyanthracene-9,10-dione and HL2 = 1-hydroxyanthracene-9,10-dione, DMSO = dimethylsulfoxide, were synthesized and fully characterized. Complex 2 was obtained through the substitution of perchlorate for DMSO. When two hydroxyquinone groups are present, L1 makes a bridge between two Cu(II) ions, which also bind two nitrogens of the respective diimine ligand.

Focal traumatic brain stem injury is a rare type of head injury resulting from assault: a forensic neuropathological study.

Introduction: Brainstem haemorrhage is common in cases of head injury when it is associated with space-occupying lesion and increases in the intracranial pressure (duret haemorrhage), in cases of diffuse axonal injury (in dorso-lateral quadrant) and diffuses vascular injury (in the periventricular tissue). However focal traumatic brainstem injury is rare.

Material And Method: We identified 12 cases of focal traumatic brainstem injury from review of 319 case of head injury.

Spectral modeling of channel band shapes in wavelength selective switches.

A model for characterizing the spectral response of the passband of Wavelength Selective Switches (WSS) is presented. We demonstrate that, in contrast to the commonly used supergaussian model, the presented model offers a more complete match to measured results, as it is based on the physical operation of the optical system. We also demonstrate that this model is better suited for calculation of WSS channel bandwidths, as well as predicting the final bandwidth of cascaded WSS modules.