Purpose: To identify the presence of endogenous superoxide anion-generating system in rabbit lens epithelial cells.
Methods: The generation of superoxide anion was quantified by using lucigenin-amplified chemiluminescence (LUCL) in intact rabbit lens epithelial cells (N/N 1003A). The LUCL readings were recorded with a luminometer (LumiStar BMG) immediately upon arachidonic acid (AA) addition.