Feasibility and Acceptability of Point-of-Care Testing for Sexually Transmitted Infections in Outpatient Clinics Offering Integrated Services in Eswatini.

Background: Lack of point-of-care testing (POCT) for sexually transmitted infections (STIs) is a continuing missed opportunity in Sub-Saharan Africa. We assessed feasibility and acceptability of STI POCT in Eswatini.

Methods: Sexually transmitted infection POCT for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) was piloted among sexually active adults 18 to 45 years old attending 2 urban outpatient clinics offering integrated services.

Thin layer chromatography-direct bioautography guided isolation of β-sitosterol from the leaves of .

The aim of the study was to isolate and characterise antimicrobial agents from the leaves of using thin-layer chromatography-direct bioautography (TLC-DB). is a medicinal plant used traditionally to treat various ailments. Previous studies have shown that species of the genus contain several classes of secondary metabolites, including sterols.

Projections of human papillomavirus vaccination and its impact on cervical cancer using the Caputo fractional derivative.

We propose a fractional order model for human papillomavirus (HPV) dynamics, including the effects of vaccination and public health education on developing cervical cancer. First, we discuss the general structure of Caputo fractional derivatives and integrals. Next, we define the fractional HPV model using Caputo derivatives.

Blood transcriptomic signatures associated with molecular changes in the brain and clinical outcomes in Parkinson's disease.

The ability to use blood to predict the outcomes of Parkinson's disease, including disease progression and cognitive and motor complications, would be of significant clinical value. We undertook bulk RNA sequencing from the caudate and putamen of postmortem Parkinson's disease (n = 35) and control (n = 40) striatum, and compared molecular profiles with clinical features and bulk RNA sequencing data obtained from antemortem peripheral blood. Cognitive and motor complications of Parkinson's disease were associated with molecular changes in the caudate (stress response) and putamen (endothelial pathways) respectively.

Determinants of complementary feeding practices among children aged 6-24 months in urban slums of Pune, Maharashtra, in India.

Background: Inequalities in child feeding practices are evident in urban slums in developing nations. Our study identified the determinants of complementary feeding (CF) practices in the informal settings of Pune, India, a district close to the business capital of India.

Methods: Employing a cross-sectional study design, 1066 mother-children dyads were surveyed.

IL-4i1 Regulation of Immune Protection During Mycobacterium tuberculosis Infection.

Background: Interleukin 4 (IL-4i1)-induced gene 1 encodes L-phenylalanine oxidase that catabolizes phenylalanine into phenylpyruvate. IL-4i1 is mainly expressed by antigen-presenting cells (APCs), inhibits T-cell proliferation, regulates B-cell activation, modulates T cell responses, and drives macrophage polarization, but its role in bacterial infections is understudied.

Methods: We evaluated IL-4i1 deletion in macrophages and mice on infection with virulent H37Rv and W-Beijing lineage hypervirulent HN878 Mycobacterium tuberculosis (Mtb) strains.

New estimations of child marriage: Evidence from 98 low- and middle-income countries.

The Sustainable Development Goals include a target on eliminating child marriage, a human rights abuse. Yet, the indicator used in the SDG framework is a summary statistic and does not provide a full picture of the incidence of marriage at different ages. This paper aims to address this limitation by providing an alternative method of measuring child marriage.

Ethics for integrating emerging technologies to contain COVID-19 in Zimbabwe.

Zimbabwe is among the countries affected with the coronavirus disease (COVID-19) and implemented several infection control and measures such as social distancing, contact tracing, regular temperature checking in strategic entry and exit points, face masking among others. The country also implemented recursive national lockdowns and curfews to reduce the virus transmission rate and its catastrophic impact. These large-scale measures are not easy to implement, adhere to and subsequently difficult to practice and maintain which lead to imperfect public compliance, especially if there is a significant impact on social and political norms, economy, and psychological wellbeing of the affected population.

Child and adolescent mental health services and systems in low and middle-income countries: from mapping to strengthening.

Purpose Of Review: Most of the world's children and adolescents live in low and middle-income countries (LMICs) where mental health services are very limited. Here, we reviewed literature over the last 18 months describing the current landscape of child and adolescent mental health services and systems (CAMHSS) in LMICs, and present findings from systems strengthening research.

Recent Findings: The challenges in CAMHSS are well described with little change over the last two decades.

Antitubercular 2-Pyrazolylpyrimidinones: Structure-Activity Relationship and Mode-of-Action Studies.

Phenotypic screening of a Medicines for Malaria Venture compound library against () identified a cluster of pan-active 2-pyrazolylpyrimidinones. The biology triage of these actives using various tool strains of suggested a novel mechanism of action. The compounds were bactericidal against replicating and retained potency against clinical isolates of .

Spatial risk assessment of an emerging pandemic under data scarcity: A case of COVID-19 in Eswatini.

Coronavirus (COVID-19) has rapidly spread across many countries in pandemic proportions since the first case was reported in Hubei, China in December 2019. Understanding transmission, susceptibility and exposure risks is crucial for surveillance, control and response to the disease. Knowing the geographic distribution of health resource scarcity areas is necessary if a country is to adequately anticipate and prepare for the full impact of infections.

Birth Testing for Infant HIV Diagnosis in Eswatini: Implementation Experience and Uptake Among Women Living With HIV in Manzini Region.

Introduction: HIV testing at birth of HIV-exposed infants (HEIs) may improve the identification of infants infected with HIV in utero and accelerate antiretroviral treatment (ART) initiation.

Methods: ICAP at Columbia University supported implementation of a national pilot of HIV testing at birth (0-7 days) in Eswatini at 2 maternity facilities. Dried blood spot (DBS) samples from neonates of women living with HIV (WLHIV) were collected and processed at the National Molecular Reference Laboratory using polymerase chain reaction (PCR).

Solvent-Free Iron(III) Chloride-Catalyzed Direct Amidation of Esters.

Amide functional groups are prominent in a broad range of organic compounds with diverse beneficial applications. In this work, we report the synthesis of these functional groups via an iron(iii) chloride-catalyzed direct amidation of esters. The reactions are conducted under solvent-free conditions and found to be compatible with a range of amine and ester substrates generating the desired amides in short reaction times and good to excellent yields at a catalyst loading of 15 mol%

The State of Adolescent Sexual and Reproductive Health.

In the 25 years since the 1994 International Conference on Population and Development, significant progress has been made in adolescent sexual and reproductive health and rights (ASRHR). Trend analysis of key ASRHR indicators at global, national, and subnational levels indicates that adolescent girls today are more likely to marry later, delay their first sexual experience, and delay their first childbirth, compared with 25 years ago; they are also more likely to use contraceptives. Despite overall progress, however, unequal progress in many ASRHR outcomes is evident both within and between countries, and in some locations, the state of adolescents' lives has worsened.

A Mathematical Model for the Transmission Dynamics of Lymphatic Filariasis with Intervention Strategies.

This manuscript considers the transmission dynamics of lymphatic filariasis with some intervention strategies in place. Unlike previously developed models, our model takes into account both the exposed and infected classes in both the human and mosquito populations, respectively. We also consider vaccinated, treated and recovered humans in the presented model.

Exploring factors that influence the integration of HIVST with HCT using a qualitative comparative cross-over design in KwaZulu-Natal, South Africa.

The World Health Organization recommends using HIV self-testing (HIVST) to improve HIV testing coverage by integrating self-testing for HIV into public domain primary healthcare (PHC) clinics in areas with a high burden of HIV. However, an understanding of the relationship and interaction between HIVST and the standard HIV counselling and testing (HCT), the primary HIV testing approach within public PHC clinics, is limited. We therefore sought to compare experiences of people seeking an HIV test, specifically, how HIVST can be used to enhance HCT when introduced together.

Repeat HIV testing practices in the era of HIV self-testing among adults in KwaZulu-Natal, South Africa.

Repeat HIV testing is important in high HIV burden communities to enable sustainability of prevention initiatives; however, an understanding of repeat testing practices is limited. Additional HIV testing approaches may be required to increase testing. HIV self-testing is an additional testing approach, but knowledge on its potential for repeat testing is limited.

HIV self-testing as part of a differentiated HIV testing approach: exploring urban and rural adult experiences from KwaZulu-Natal, South Africa using a cross-over study design.

Background: Suboptimal HIV testing rates through available testing approaches such as HIV counselling and testing have directed research efforts toward recognizing the potential of HIV self-testing as an additional testing method. However, HIV self-testing is not readily available within HIV testing facilities and data on how HIV self-testing and HIV counselling and testing will co-exist within HIV testing facilities is limited. Therefore, this study sought to fill this knowledge gap.

An investigation of the fate and behaviour of a mixture of WO and TiO nanoparticles in a wastewater treatment plant.

The fate and behaviour of WO and TiO mixture were investigated following the Organisation for Economic Co-operation and Development 303A guidelines. The nanoparticles were found not to influence the chemical oxygen demand removal efficiency which was maintained >80% hence the activated sludge process was on affected. The nanoparticles were eliminated from the wastewater with a greater percentage of 99.

Integrating cardiovascular disease risk factor screening into HIV services in Swaziland: lessons from an implementation science study.

Objective: To study the feasibility of cardiovascular disease risk factor (CVDRF) screening at an HIV clinic in Swaziland.

Methods: A sample of HIV-positive patients at least 40 years on antiretroviral treatment was screened for hypertension, diabetes, hyperlipidemia, and tobacco smoking.

Results: A total of 1826 patients were screened; 684 (39%) had at least one CVDRF.

A time-motion study of cardiovascular disease risk factor screening integrated into HIV clinic visits in Swaziland.

Introduction: Screening of modifiable cardiovascular disease (CVD) risk factors is recommended but not routinely provided for HIV-infected patients, especially in low-resource settings. Potential concerns include limited staff time and low patient acceptability, but little empirical data exists. As part of a pilot study of screening in a large urban HIV clinic in Swaziland, we conducted a time-motion study to assess the impact of screening on patient flow and HIV service delivery and exit interviews to assess patient acceptability.

Novel Antitubercular 6-Dialkylaminopyrimidine Carboxamides from Phenotypic Whole-Cell High Throughput Screening of a SoftFocus Library: Structure-Activity Relationship and Target Identification Studies.

A BioFocus DPI SoftFocus library of ∼35 000 compounds was screened against Mycobacterium tuberculosis (Mtb) in order to identify novel hits with antitubercular activity. The hits were evaluated in biology triage assays to exclude compounds suggested to function via frequently encountered promiscuous mechanisms of action including inhibition of the QcrB subunit of the cytochrome bc complex, disruption of cell-wall homeostasis, and DNA damage. Among the hits that passed this screening cascade, a 6-dialkylaminopyrimidine carboxamide series was prioritized for hit to lead optimization.

Microscale Gaseous Slip Flow in the Insect Trachea and Tracheoles.

An analytical investigation into compressible gas flow with slight rarefactions through the insect trachea and tracheoles during the closed spiracle phase is undertaken, and a complete set of asymptotic analytical solutions is presented. We first obtain estimates of the Reynolds and Mach numbers at the channel terminal ends where the tracheoles directly deliver respiratory gases to the cells, by comparing the magnitude of the different forces in the compressible gas flow. The 2D Navier-Stokes equations with a slip boundary condition are used to investigate compressibility and rarefied effects in the trachea and tracheoles.

Mathematical analysis of a lymphatic filariasis model with quarantine and treatment.

Background: Lymphatic filariasis is a globally neglected tropical parasitic disease which affects individuals of all ages and leads to an altered lymphatic system and abnormal enlargement of body parts.

Methods: A mathematical model of lymphatic filariaris with intervention strategies is developed and analyzed. Control of infections is analyzed within the model through medical treatment of infected-acute individuals and quarantine of infected-chronic individuals.