Publications by authors named "Silvie Ostrizkova"
Background: Lung carcinoma is one of the most common malignancies worldwide. At present, unfortunately, there are no markers that would allow early identification of this tumor in the preclinical or early clinical stage. The use of sniffer dogs has been reported to show some promise in early diagnosis of this type of cancer Aim: This study aimed to evaluate the possibility of utilizing changes in the heart rate of sniffer dogs (which increases when finding a positive sample) in tumor detection.
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- - The study explored how effective the ddPCR instrument is for analyzing DNA from low-quality saliva samples, compared to traditional blood samples, since saliva collection is easier and less invasive.
- - Researchers collected blood and saliva from 47 university students, extracted DNA, and performed analyses using ddPCR, thereby assessing the quality of the isolates.
- - Results showed that ddPCR effectively analyzed even low-concentration saliva samples, achieving 100% agreement with blood sample results, indicating its potential for use in large-scale genetic studies despite poor sample quality.
Background: Thrombophilic mutations in genes for factor V Leiden and factor II prothrombin are among the most important risk factors for developing the thromboembolic disease (TED), along with the use of oral contraceptives (OCs) or smoking.
Aim: This study aimed to investigate the occurrence of risk factors in young women using droplet digital PCR (ddPCR) and, based on the results of this investigation, to perform a cost-benefit analysis of ddPCR-based screening in young women starting to take OCs compared to the treatment costs of patients who develop preventable TED in the Czech Republic.
Methods: In this cross-sectional study, female university students filled in a questionnaire and provided a blood sample for DNA isolation and ddPCR analysis of both aforementioned genetic risk factors.