Publications by authors named "Silviatun Nihayah"
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease 9 (CRISPR/Cas9) offers a robust approach for genome manipulation, particularly in cancer therapy. Given its high expression in triple-negative breast cancer (TNBC), targeting with CRISPR/Cas9 holds promise as a therapeutic strategy. The aim of this study was to design specific single guide ribonucleic acid (sgRNA) for CRISPR/Cas9 to permanently knock out the gene, exploring its potential as a therapeutic approach in breast cancer while addressing potential off-target effects.
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- * Traditional methods for studying DNA methylation are invasive, so this study explores using buccal (cheek) cells as a less invasive alternative to collect biomarkers related to stunting.
- * Using adaptive sampling from Oxford Nanopore Technology, researchers found that stunted children had lower levels of 5-methylcytosine and identified specific gene loci on chromosome 21 that are differentially methylated and previously unassociated with stunting.
The prevalence of stunting in Indonesian children under five years of age is about 20%. Chronic maternal malnutrition contributes to the risk of stunting by affecting global DNA methylation. In the present study, we aimed to evaluate the levels of 5-methyl-cytosine (5mC), as a surrogate marker of global DNA methylation, in buccal swabs and its potential association with risk of stunting and cognitive performance.
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