Alterations of the gene and the resulting changes in the BRAF protein are one example of molecular cancer profiling in humans and dogs. We tested 227 samples of canine carcinomas from different anatomical sites (anal sac ( = 23), intestine ( = 21), liver ( = 21), lungs ( = 19), mammary gland ( = 20), nasal cavity ( = 21), oral epithelium ( = 18), ovary ( = 20), prostate ( = 21), thyroid gland ( = 21), urinary bladder ( = 22)) with two commercially available primary anti-BRAF antibodies (VE1 Ventana, VE1 Abcam). The immunohistochemical results were confirmed with droplet digital PCR (ddPCR).
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