Genetic alterations of IDH1 and Vegf in brain tumors.

Background: This study evaluates the presence of R132H mutation in isocitrate dehydrogenase () gene and the vascular endothelial growth factor () +936 C/T polymorphism in brain tumors. The impact of these genetic alterations on overall survival (OS) and progression free survival (PFS) was evaluated.

Methods: A cohort of 80 patients surgically treated at Hospital Clínico San Carlos, Madrid, between March 2004 and November 2012, was analyzed.

Effect of β-catenin alterations in the prognosis of patients with sporadic colorectal cancer.

Context: Wnt pathway activation represents a critical step in the etiology of most of colorectal cancer (CRC) and it is commonly due to mutations in the APC gene, which originates the loss of β-catenin regulatory function. It has been suggested that APC inactivation or β-catenin alteration have similar effects in tumor progression in CRC tumorigenesis.

Aims: The aim of this study was to analyze the frequency of β-catenin gene mutation in patients with sporadic CRC and to determine its effect in prognosis.

seqCNA: an R package for DNA copy number analysis in cancer using high-throughput sequencing.

Background: Deviations in the amount of genomic content that arise during tumorigenesis, called copy number alterations, are structural rearrangements that can critically affect gene expression patterns. Additionally, copy number alteration profiles allow insight into cancer discrimination, progression and complexity. On data obtained from high-throughput sequencing, improving quality through GC bias correction and keeping false positives to a minimum help build reliable copy number alteration profiles.

A9 region in EPHB2 mutation is frequent in tumors with microsatellite instability. Analysis of prognosis.

Aim: The aim of the present study was to determine the relation of EPH tyrosine kinase receptor B2 (EPHB2) A9 region mutation and microsatellite instability (MSI); and to analyze their influence in prognosis of patients with sporadic colorectal cancer (CRC).

Patients And Methods: A total of 481 patients with CRC were examined. MSI (NCI criteria) and EPHB2 were analyzed using PCR and fragment analysis software.

Serum level of fibroblast growth factor 23 in maintenance renal transplant patients.

Background: The discovery of fibroblast growth factor 23 (FGF23) provides a new conceptual framework that improves our understanding of the pathogenesis of post-transplant bone disease. Excess FGF23 is produced in the early post-transplant period; levels return to normal in the months following transplant. However, few manuscripts discuss FGF23 levels in stable long-term renal transplant recipients.

Circulating tumor cells in solid tumor in metastatic and localized stages.

Unlabelled: The aim of this study was the detection of circulating tumor cells (CTC) in three tumor types of epithelial origin.

Patients And Methods: Four hundred and thirty-eight patients with breast cancer (56.2% localized and 43.

Circulating tumor cells in metastatic breast cancer: timing of blood extraction for analysis.

Purpose: Circulating tumor cells (CTCs) can be detected in the peripheral blood of around 50% of patients with metastatic breast cancer. Their numbers are an independent predictor of the patient's progression-free survival (PFS) and of overall survival (OS). However, to date, none of the studies carried out with the most commonly used system of CTC determination (the CellSearch System, approved by the US Food and Drug Administration) has examined the intra-patient variation in CTC numbers, a variation that could impact on prognosis assessment.

Circulating tumour cells in locally advanced breast cancer.

Material And Methods: A prospective study was conducted to determine the value of changes in circulating tumour cell (CTC) levels prior to and after the first cycle of neoadjuvant treatment in early prediction of pathologic response in locally advanced breast cancer (LABC). Two blood samples were obtained from 72 eligible LABC patients to isolate and enumerate CTCs before neoadjuvant chemotherapy started on day 1, and on day 21, immediately before second cycle administration.

Results: Sixty patients (83.

Inactivation of p16 by CpG hypermethylation in renal cell carcinoma.

Objective: Renal carcinoma develops as a consequence of the accumulation of several genetic aberrations. Alterations in the p16 gene have been described in many tumors. Methylation of its promoter in CpG islands is the most common mechanism of inactivation of this gene.

Circulating tumoral cells lack circadian-rhythm in hospitalized metastasic breast cancer patients.

Background: The relationship between breast cancer and circadian rhythm variation has been extensively studied. Increased breast tumorigenesis has been reported in melatonin-suppressed experimental models and in observational studies.

Objectives: Circulating Tumor Cells (CTC) circadian- rhythm may optimize the timing of therapies.