Measurement of serum monoclonal components: comparison between densitometry and capillary zone electrophoresis.

Quantitative measurement of serum monoclonal protein (M-protein) is one of the most important tools for monitoring disease activity in monoclonal gammopathies. The aims of this study were to evaluate serum M-protein quantification by capillary zone electrophoresis (CZE) and to compare results with those obtained by densitometric scanning of high-resolution agarose gel electrophoresis (HRE-AGE). The evaluation was carried out on 82 samples from patients with various monoclonal gammopathies.

Evaluation of a new capillary zone electrophoresis system for the identification and typing of Bence Jones Protein.

Objectives: Capillary electrophoresis has recently emerged as a new sensitive technique for the separation of urinary proteins. We evaluated a new method for Bence Jones Protein (BJP) detection and characterization on native urine samples by the Paragon CZE 2000 system. To avoid interference in electrophoretic separation, urine samples were preliminarily treated for the selective removal of interfering salt particles.