Molecular Mechanisms of Resistance to Ionizing Radiation in and Its Relationship with Aging, Oxidative Stress, and Antioxidant Activity.

The repair of the damage produced to the genome and proteome by the action of ionizing radiation, oxidizing agents, and during aging is important to maintain cellular homeostasis. Many of the metabolic pathways influence multiple processes. In this way, this work aims to study the relationship between resistance/response to ionizing radiation, cellular aging, and the response mechanisms to oxidative stress, free radicals, reactive oxygen species (ROS), and antioxidant activity in the yeast .

Microbiome Alterations and Alzheimer's Disease: Modeling Strategies with Transgenic Mice.

In the last decade, the role of the microbiota-gut-brain axis has been gaining momentum in the context of many neurodegenerative and metabolic disorders, including Alzheimer's disease (AD) and diabetes, respectively. Notably, a balanced gut microbiota contributes to the epithelial intestinal barrier maintenance, modulates the host immune system, and releases neurotransmitters and/or neuroprotective short-chain fatty acids. However, dysbiosis may provoke immune dysregulation, impacting neuroinflammation through peripheral-central immune communication.

Pulsed magnetic field increases the effect of ultraviolet C radiation and thermal shock in aged yeasts.

The study of the effects of the magnetic field (MF) on living matter continues to be a dilemma. Until now, the interaction mechanisms of MF with living matter that explain the observed phenomena are unknown. Despite the existing literature and the multiple effects described to date, there are few published articles that study the combined effect of MF with other physical agents during the cellular aging process.

Genomic DNA damage induced by co-exposure to DNA damaging agents and pulsed magnetic field.

Purpose: Many articles describe the effects of extremely low-frequency magnetic fields (MFs) on DNA damage induction. However, the mechanism of MF interaction with living matter is not yet known with certainty. Some works suggest that MF could induce an increase in the efficacy of reactive oxygen species (ROS) production.

Growth alteration of L. roots exposed to 1.5 mT, 25 Hz pulsed magnetic field.

The response of plants to magnetic fields (MF) is not fully understood. This work studies the effects of pulsed MF on the germination and growth of roots. Onions were exposed to 25Hz, 1.

Exposure of to pulsed magnetic field during chronological aging could induce genomic DNA damage.

This study evaluates the DNA damage induced by pulsed magnetic field (MF) on cells exposed during chronological aging. Samples were exposed to 25 Hz pulsed MF (1.5mT, 8 h/day) while cells were aging chronologically.

Identification of new proteins related with cisplatin resistance in Saccharomyces cerevisiae.

The aim of this study is to select a cisplatin-resistant Saccharomyces cerevisiae strain to look for new molecular markers of resistance and the identification of mechanisms/interactions involved. A resistant strain was obtained after 80 days of cisplatin exposure. Then, total protein extraction, purification, and identification were carried out, in wild-type (wt) and resistant strains, by tandem mass spectrometry using a "nano HPLC-ESI-MS/MS" ion trap system.

Effect of low frequency magnetic field on efficiency of chromosome break repair.

DNA repair is essential to maintain genome integrity. There is scientific evidence that exposure to magnetic fields (MF) can produce alterations in DNA repair without clear conclusions. This work aims to study the cellular response to and repair of a very deleterious type of DNA damage, the DNA double strand break (DSB), in , under MF exposure.

Effect of sinusoidal and pulsed magnetic field exposure on the chronological aging and cellular stability of .

The aim of this study is to investigate the effects of low frequency and intensity sinusoidal magnetic field (SMF) and pulsed magnetic field (PMF) exposure on the chronological aging and cellular stability of Saccharomyces . The wild type strain (WS8105-1C) was exposed to SMF (2.45 mT, 50 Hz, continuous) and PMF (1.

Inactivation of RAD52 and HDF1 DNA repair genes leads to premature chronological aging and cellular instability.

The present study aims to investigate the role of radiation sensitive 52 (RAD52) and high-affinity DNA binding factor 1 (HDF1) DNA repair genes on the life span of budding yeasts during chronological aging. Wild type (wt) and rad52, hdf1, and rad52 hdf1 mutant Saccharomyces cerevisiae strains were used. Chronological aging and survival assays were studied by clonogenic assay and drop test.

Long-term exposure to a pulsed magnetic field (1.5 mT, 25 Hz) increases genomic DNA spontaneous degradation.

The aim of this work is to investigate whether long-term pulsed magnetic field (MF) has genotoxic activity by induction of DNA damage on DNA molecules in vitro, in the absence of repair mechanisms. Yeast genomic DNA prepared by phenol extraction from S. cerevisiae cultures and the commercial DNA molecular weight marker Hyperladder I (HL-I) were exposed to 1.