Anadenanthera colubrina regulated LPS-induced inflammation by suppressing NF-κB and p38-MAPK signaling pathways.

We aimed to determine the chemical profile and unveil Anadenanthera colubrina (Vell.) Brenan standardized extract effects on inflammatory cytokines expression and key proteins from immunoregulating signaling pathways on LPS-induced THP-1 monocyte. Using the RT-PCR and Luminex Assays, we planned to show the gene expression and the levels of IL-8, IL-1β, and IL-10 inflammatory cytokines.

Development of nanobiosilicate, tricalcium phosphate and chlorhexidine materials for biomineralization with crystallographic similarity to hydroxyapatite and biomodified collagen.

Objectives: The aim of this work is to test experimental cements, doped with a silicate based bioactive nanoparticle (NanoBiosilicate). Methods, we synthesized a glass nanoparticle by Sol-Gel Stöber method, used to be incorporated in a dental material for endodontic uses.

Materials And Methods: We assess the mineralizing properties and biocompatibility.

Outer Membrane Proteins 29 and 29 Paralogue Induce Evasion of Immune Response.

() is abundant within the microbial dysbiotic community of some patients with periodontitis. outer membrane protein 29 (OMP29), a member of the OMPA family, mediates the invasion of to gingival epithelial cells (GECs). This study evaluated the effect of OMP29 and its paralogue OMP29 on the response of GECs to .

Yeast-Host Interactions: Modulates Virulence Factors of and Inflammatory Response .

Oral candidiasis is one of the most common fungal infections in humans. Its incidence has increased widely, as well as the antifungal resistance, demanding for the search for novel antifungal therapeutic agents. (Vell.

(Vell.) Brenan as an inhibitor of HIV-1 BaL infection.

We reported the anti-HIV-1 activity, cytotoxicity, cytokines expression and chemical profile of . Cytotoxicity was evaluated on TZM-bl, HL2/3 cells and macrophages. Anti-HIV-1 activity was determined by Luciferase assay (TZM-bl cells) and by HIV-p24 quantification (macrophages) assessed by ELISA.

derivatives as inhibitors of HIV-1 BaL infection.

The emphasis of the present study is to evaluate a natural product and the potential microbicide activity using a dual chamber infection method. extracts and fractions were screened for anti-HIV activity by measuring the virus-antibody neutralization. Plant extracts with strong antiviral activity working in nanomolar or picomolar range can be used to enhance the activity of synthetic compounds and work as anti-HIV agents.

Effects of Monolaurin on Oral Microbe-Host Transcriptome and Metabolome.

The aim of this study was to evaluate the effects of monolaurin against (Aa) and determine their effects on the host transcriptome and metabolome, using an oral cell/bacteria co-culture dual-chamber model to mimic the human periodontium. For this, the Aa, was applied to cross the monolayer of epithelial keratinocytes (OBA-9) to reach the fibroblasts layer (HGF-1) in the basal chamber. The Monolaurin treatments (25 or 50 μM) were added immediately after the inoculation of the dual-chamber with Aa.

Fungal-Host Interaction: Curcumin Modulates Proteolytic Enzyme Activity of and Inflammatory Host Response .

Current treatments for infection are limited due to the limited number of antifungal drugs available and the increase in antifungal resistance. Curcumin is used as a spice, food preservative, flavoring, and coloring agent that has been shown to have many pharmacological activities. Thus, this study evaluated the modulatory effects of curcumin on major virulence factors associated with the pathogenicity of .

Anti-HIV-1 activity of flavonoid myricetin on HIV-1 infection in a dual-chamber in vitro model.

HIV infection by sexual transmission remains an enormous global health concern. More than 1 million new infections among women occur annually. Microbicides represent a promising prevention strategy that women can easily control.

TLR2 and TLR4 gene promoter methylation status during chronic periodontitis.

Aim: The objective of this study was to analyse the status of DNA methylation in the promoter region of the toll-like receptor (TLR)2 and TLR4 genes in gingival tissue samples from healthy subjects, smokers and non-smokers affected by chronic periodontitis.

Material And Methods: Genomic DNA and total RNA were purified from gingival tissue using the TRIZOL reagent protocol. Genomic DNA was then digested by methylation-sensitive restriction enzymes, amplified by polymerase chain reaction (PCR), electrophoresed on a 10% polyacrylamide gel and stained using SYBR Gold.

Latex use as an occlusive membrane for guided bone regeneration.

Latex extracted from Hevea brasiliensis was used as an occlusive membrane for guided bone regeneration. Twenty-four rabbits were divided in two groups: treated and control group. Critical size bone defects (2 cm × 1 cm) were surgically made in the rabbit calvarium.

Effects of maternal ethanol intake on immunoexpression of epidermal growth factor in developing rat mandibular molar.

Objective: A polyclonal antibody was used to investigate the effects of ethanol ingestion before and during pregnancy, in the expression of EGF on dentinogenesis and amelogenesis of rat mandibular first molar.

Design: Ethanol was administered to drinking water (treated group) starting at concentrations of 1% and increasing weekly to 5, 10, 15, 20 and 25% (v/v). During week 7, these rats were mated and continued to receive the 25% alcoholic solution, up to delivery.