Physicochemical Profile of Canastra Cheese Inoculated with Starter Cultures of and .

Canastra cheese, an artisanal cheese produced in Serra da Canastra-Brazil, has great cultural importance. Furthermore, this cheese has nutritional and sensory attributes that make it of great economic importance. Its microbiota is composed of different bacteria and yeasts.

Phytotoxic Activity Analysis of 2-Methoxyphenol and 2,6-Di--butyl-4-methylphenol Present in L. Essential Oil.

The evaluation of the wide variety of allelochemicals present in allelopathic plants allows the detection of safer bioherbicides with new mechanisms of action. This study tested two phenolic compounds of essential oil (2-Methoxyphenol and 2,6-Di--butyl-4-methylphenol), which are commercially available. At 0.

Ecological dynamics of Enterobacteriaceae in the human gut microbiome across global populations.

Gut bacteria from the Enterobacteriaceae family are a major cause of opportunistic infections worldwide. Given their prevalence among healthy human gut microbiomes, interspecies interactions may play a role in modulating infection resistance. Here we uncover global ecological patterns linked to Enterobacteriaceae colonization and abundance by leveraging a large-scale dataset of 12,238 public human gut metagenomes spanning 45 countries.

Finding the balance: Advancing strategies for managing severely calcified coronary lesions.

This letter to the editor provides a critical and constructive analysis of the article "Intravascular Lithotripsy Compared with Rotational Atherectomy for Calcified Coronary Lesions: A Meta-analysis of Outcomes", highlighting key methodological limitations and the exclusion of relevant contemporary studies. It emphasizes the clinical importance of addressing severely calcified coronary lesions, a significant challenge in interventional cardiology, and advocates for future research to prioritize randomized clinical trials, subgroup analyses, and cost-effectiveness evaluations to improve the applicability of findings across healthcare settings. By promoting dialogue within the scientific community and encouraging the integration of evolving data, the letter aims to refine clinical strategies and align them with evidence-based public health approaches, particularly in resource-limited environments.

Proteolytic profile and nematicidal potential of proteases produced by Pleurotus djamor in coprocultures.

The aim of this study was to evaluate the proteolytic profile of the cell-free crude extract (CFCE) of Pleurotus djamor and its nematicidal action on Haemonchus spp. and Trichostrongylus spp. larvae in coprocultures.

Multi-omics integration and immune profiling identify possible causal networks leading to uterine microbiome dysbiosis in dairy cows that develop metritis.

Background: Cows that develop metritis experience dysbiosis of their uterine microbiome, where opportunistic pathogens overtake uterine commensals. An effective immune response is critical for maintaining uterine health. Nonetheless, periparturient cows experience immune dysregulation, which seems to be intensified by prepartum over-condition.

Effectiveness of Integrated Digital Solutions to Empower Older Adults in Aspects Related to Their Health: Systematic Review and Meta-Analysis.

Background: Digital solutions, such as mobile apps or telemonitoring devices, are frequently considered facilitators in the process of empowering older adults, but they can also act as a source of digital exclusion or disempowerment if they are not adequate for older adults' needs and characteristics.

Objective: This study aimed to synthesize and critically evaluate existing evidence on the effectiveness of integrated digital solutions that enable interaction for empowering older adults in aspects related to their health and to explore potential factors (eg, type of technology, participants' characteristics) impacting effectiveness.

Methods: A systematic search was carried out in PubMed, ScienceDirect, SCOPUS, EBSCO, and SciELO using a combination of terms informed by previous reviews on empowerment.

Biological silage from tambaqui (Colossoma macropomum) by-products on the productive performance, hematological parameters and egg quality of older commercial hens.

This study evaluated the effects of incorporating biological silage from tambaqui (Colossoma macropomum) by-products (BST) on the performance, hematological and plasma biochemical parameters, and egg quality (physical, proximate composition and sensory characteristics) of older commercial hens. The BST was prepared by ensiling tambaqui by-products with lactic acid bacteria, cassava trimmings, and preservatives, producing a nutrient-rich, high-protein feed ingredient. One hundred and twenty Hisex Brown hens (83 weeks old) were divided into five treatment groups, each receiving diets with 0, 1, 2, 3, or 4% BST.

Pons herniation after skull base chondrosarcoma surgery: A rare complication after transclival endoscopic endonasal approach.

Background: Intracranial chondrosarcomas are malignant tumors that most commonly affect the clivus region. For tumors in this location, the extended endoscopic endonasal approach could offer an alternative route. We present a case of pons herniation after this technique.

A modified orthogonal-distance ray-tracer method applied to dual rotation PET systems.

A new projector, Orthogonal-Distance Ray-tracer Varying-Full Width at Half Maximum (OD-RT-VF), was developed to model a shift-variant elliptical point-spread function (PSF) response to improve the image quality of a preclinical dual-rotation PET system. Approach: The OD-RT-VF projector models different FWHM values of the PSF in multiple directions, using half-height and half-width tube-of-response (ToR) values. The OD-RT-VF method's performance was evaluated against the original OD-RT method and a ToR model with constant response.

Newly Proposed Dose of Daclatasvir to Prevent Lethal SARS-CoV-2 Infection in Human Transgenic ACE-2 Mice.

Coronavirus disease 2019 (COVID-19) still causes death in elderly and immunocompromised individuals, for whom the sustainability of the vaccine response may be limited. Antiviral treatments, such as remdesivir or molnupiravir, have demonstrated limited clinical efficacy. Nirmatrelvir, an acute respiratory syndrome coronavirus 2 (SARS-CoV-2) major protease inhibitor, is clinically effective but has been associated with viral rebound and antiviral resistance.

-Derived β-Glucan Capsules as a Delivery System for DNA Vaccines.

Background/objectives: DNA vaccines are rapidly produced and adaptable to different pathogens, but they face considerable challenges regarding stability and delivery to the cellular target. Thus, effective delivery methods are essential for the success of these vaccines. Here, we evaluated the efficacy of capsules derived from the cell wall of the yeast as a delivery system for DNA vaccines.

Longitudinal Immunological Analysis of Portuguese Healthcare Workers Across the COVID-19 Pandemic Reveals Differences in the Humoral Immune Response to Vaccines.

A vaccination programme against severe acute respiratory syndrome coronavirus 2 was initiated in Portugal in December 2020. In this study, we report the findings of a prospective cohort study implemented with the objective of monitoring antibody production in response to COVID-19 vaccination. The humoral immune response to vaccination was followed up using blood samples collected from 191 healthcare workers.

Upcity: Addressing Urban Problems Through an Integrated System.

Current technologies could potentially solve many of the urban problems in today's cities. Many cities already possess cameras, drones, thermometers, pollution air gauges, and other sensors. However, most of these have been designated for use in individual domains within City Hall, creating a maze of individual data domains that cannot connect to each other.

Advances in Encapsulating Marine Bioactive Compounds Using Nanostructured Lipid Carriers (NLCs) and Solid Lipid Nanoparticles (SLNs) for Health Applications.

As life expectancy rises and modern lifestyles improve, there is an increasing focus on health, disease prevention, and enhancing physical appearance. Consumers are more aware of the benefits of natural ingredients in healthcare products while also being mindful of sustainability challenges. Consequently, marine bioactive compounds have gained popularity as ingredients in cosmetics and food supplements due to their diverse beneficial properties.

Exploring Novel Fungal-Bacterial Consortia for Enhanced Petroleum Hydrocarbon Degradation.

Bioremediation, involving the strategic use of microorganisms, has proven to be a cost-effective alternative for restoring areas impacted by persistent contaminants such as polycyclic aromatic hydrocarbons (PAHs). In this context, the aim of this study was to explore hydrocarbon-degrading microbial consortia by prospecting native species from soils contaminated with blends of diesel and biodiesel (20% biodiesel/80% diesel). After enrichment in a minimal medium containing diesel oil as the sole carbon source and based on 16S rRNA, Calmodulin and β-tubulin gene sequencing, seven fungi and 12 bacteria were identified.

Antinociceptive Potential of L. Bark Extract and Caffeic Acid: Insights into Pain Modulation Pathways.

This study evaluated the antinociceptive effect of the L. bark extract (HEXA) and its primary component, caffeic acid (CA), through in vivo assays. : The antinociceptive properties were assessed using abdominal writhing, hot plate, and Von Frey tests.

Phytochemical Study and In Vitro Antioxidant Activity of Along with Antitumor Activity of the Isolated -Tiliroside and Luteolin 4'--β-Xyloside.

Twelve compounds (-), kaempferol (), luteolin (), luteolin 4'--xyloside (), luteolin 4'--β-glucoside (), quercetin 4'--β-xyloside (), kaempferol-3--[6″--(E)-p-coumaroyl]-β-D-glucoside (-tiliroside) (), protocatechuic acid (), gallic acid (), methyl gallate (), ethyl gallate (), shikimic acid-3--gallate (), and 3,3',4'-tri--methyl-ellagic acid 4-sulfate (), were isolated and identified from the aerial parts of (Cav.) Pers (synonym: C. Presl.

Larvicidal and Oviposition Activity of Commercial Essential Oils of Ledeb., (Blanco) Benth., L. and Their Combinations Against .

is a vector responsible for the transmission of various arboviruses and is considered by the World Health Organization to be one of the main public health problems in the world. This study evaluated the larvicidal and oviposition activity of essential oils from , and and their formulations. Chromatographic analysis by GCMS identified a total of 28, 52 and 18 compounds for the oils of the species , and , respectively.

Structural Characterization and In Vitro and In Silico Studies on the Anti--Glucosidase Activity of Anacardic Acids from .

The growing focus on sustainable use of natural resources has brought attention to cashew nut shell liquid (CNSL), a by-product rich in anacardic acids (AAs) with potential applications in diabetes treatment. In this study, three different AAs from CNSL, monoene (15:1, AAn1), diene (15:2, AAn2), and triene (15:3, AAn3), and a mixture of the three (mix) were evaluated as -glucosidase inhibitors. The samples were characterized by combining 1D and 2D NMR spectroscopy, along with ESI-MS.

Increasing the Construct Validity of Computational Phenotypes of Mental Illness Through Active Inference and Brain Imaging.

After more than 30 years since its inception, the utility of brain imaging for understanding and diagnosing mental illnesses is in doubt, receiving well-grounded criticisms from clinical practitioners. Symptom-based correlational approaches have struggled to provide psychiatry with reliable brain-imaging metrics. However, the emergence of computational psychiatry has paved a new path not only for understanding the psychopathology of mental illness but also to provide practical tools for clinical practice in terms of computational metrics, specifically computational phenotypes.

Therapeutic Potential of Dimethyl Fumarate for the Treatment of High-Fat/High-Sucrose Diet-Induced Obesity.

Obesity is characterized by an imbalance between energy intake and expenditure that triggers abnormal growth of adipose tissues. Dimethyl fumarate (DMF) and its primary active metabolite, monomethyl fumarate (MMF), are Nrf2 activators and have been recognized as strategic antioxidants. This study aimed to evaluate the potential of MMF and DMF to interfere with adipogenesis and obesity, and identify the molecular mechanisms involved.

The Effects of a Blend of Essential Oils in the Milk of Suckling Calves on Performance, Immune and Antioxidant Systems, and Intestinal Microbiota.

The objective of the present study was to determine whether the addition of a blend based on the essential oils of cinnamon, oregano, and eucalyptus to the liquid diets of calves would stimulate the immune system combined with anti-inflammatory action, minimize oxidative responses, and alter the intestinal microbiota, consequently enhancing animal growth. Twenty-four male Holstein calves (approximately five days old) were suckled for 60 days, underwent a weaning process, and were followed up until day 75 of the experiment. The calves were divided into control ( = 12) and phytobiotic ( = 12) groups, receiving commercial milk replacer and pelleted concentrate ad libitum.