Improvement of antioxidant capacity, aroma quality, and antifungal ability of cherry by phenyllactic acid treatment during low temperature storage.

Introduction: Sweet cherries ( L.) are highly valued for their taste and nutrients but are prone to decay due to their delicate skin and high respiration rate. Traditional chemical preservatives have drawbacks like residues and resistance, prompting the search for natural alternatives.

Calcium channels as therapeutic targets in head and neck squamous cell carcinoma: current evidence and clinical trials.

Head and neck squamous cell carcinoma (HNSCC) originates from the mucosal epithelium of the oral cavity, pharynx, and larynx, and is marked by high rates of recurrence and metastasis. Calcium signaling is associated with the progression of HNSCC and the development of drug resistance. Changes in calcium ion flow can trigger severe pathophysiological processes, including malignant transformation, tumor proliferation, epithelial-mesenchymal transition, and apoptosis evasion.

Successful resection of a huge brainstem enterogenous cyst: case report and literature review.

Enterogenous cysts (ECs) are rare, benign, congenital ectopic endodermal cysts that only occasionally involve the central nervous system. We presented the diagnosis and treatment of an exceedingly rare case of EC located in the brainstem, which has previously been reported only seven times in pediatric patients. The patient underwent complete surgical resection and experienced no recurrence during the 6-month follow-up.

Combined use of super-resolution ultrasound imaging and shear-wave elastography for differential diagnosis of breast masses.

Objectives: Shear-wave elastography (SWE) provides valuable stiffness within breast masses, making it a useful supplement to conventional ultrasound imaging. Super-resolution ultrasound (SRUS) imaging enhances microvascular visualization, aiding in the differential diagnosis of breast masses. Current clinical ultrasound diagnosis of breast cancer primarily relies on gray-scale ultrasound.

Single-cell transcriptomic analysis of chondrocytes in cartilage and pathogenesis of osteoarthritis.

Chondrocyte is considered the only cell type in cartilage. However, the cell heterogeneity of chondrocytes in human articular cartilage is still not well defined, which hinders our understanding of the pathogenesis of osteoarthritis (OA). Here, we constructed a single-cell transcriptomic atlas of chondrocytes in healthy cartilage and identified nine chondrocyte subsets including homeostatic chondrocytes, proliferate fibrochondrocytes, and hypertrophic chondrocytes (HTC).

Analysis of risk factors for abnormal renal function in patients with gout in Southwest China: a cross-sectional study.

Objective: To analyse the associations between renal function and clinical laboratory indicators and explore the renal function abnormality risk factors for gout patients in Southwest China.

Methods: Outpatient and hospitalized gout patients ( = 4384) at the First Affiliated Hospital of Chengdu Medical College between January 2017 and December 2020 were divided into normal ( = 2393) and abnormal ( = 1991) renal function groups according to their eGFR. The relationships between clinical laboratory indicators and the eGFR were analysed, and a logistic regression model was fit to identify significant risk factors.

Evaluation of the ability of large language models to self-diagnose oral diseases.

Large language models (LLMs) offer potential in primary dental care. We conducted an evaluation of LLMs' diagnostic capabilities across various oral diseases and contexts. All LLMs showed diagnostic capabilities for temporomandibular joint disorders, periodontal disease, dental caries, and malocclusion.

Design, synthesis, anticancer activity and molecular docking of quinoline-based dihydrazone derivatives.

Based on the biologically active heterocycle quinoline, we successfully synthesized a series of quinoline-based dihydrazone derivatives (3a-3d). H NMR, C NMR, ESI-HRMS, IR, element analysis, UV/Vis spectroscopy and fluorescence spectroscopy were performed to comprehensively characterize their chemical structures, spectral properties and stability. Nitrosamine impurities were not detected in 3a-3d, and the systemic toxicological assessment indicated that the toxicity of 3a-3d was lower.

Optimization Control Method for Low-Voltage DC Microgrid with Low Carbon, Economy, and Reliability.

From the perspectives of economy, low carbon, and safety in DC microgrids, a multiscenario optimization control method of low-voltage DC microgrids based on the nondominant sorting arctic puffin optimization algorithm (NSAPOA) is proposed in this paper. The Wasserstein generative adversarial network with gradient penalty (WGAN-GP) is used to generate typical output scenarios of photovoltaic and loads that are reduced by the K-means clustering method to deal with the uncertainty of photovoltaic and load. Based on the time of use electricity price, the operating modes of the low-voltage DC microgrid system are divided to formulate relevant energy exchange strategies.

Tumor burden with AFP improves survival prediction for TACE-treated patients with HCC: An international observational study.

Background & Aims: Current prognostic models for patients with hepatocellular carcinoma (HCC) undergoing transarterial chemoembolization (TACE) are not extensively validated and widely accepted. We aimed to develop and validate a continuous model incorporating tumor burden and biology for individual survival prediction and risk stratification.

Methods: Overall, 4,377 treatment-naive candidates for whom TACE was recommended, from 39 centers in five countries, were enrolled and divided into training, internal validation, and two external validation datasets.

Enhancing smart healthcare networks: Integrating attribute-based encryption for optimization and anti-corruption mechanisms.

This study investigates the feasibility and effectiveness of integrating Attribute-Based Encryption (ABE) into smart healthcare networks, with a particular focus on its role in enhancing anti-corruption mechanisms. The study provides a comprehensive analysis of current vulnerabilities in these networks, identifying potential data security risks. An anti-corruption mechanism is designed to ensure data integrity and reliability.

Trends in Obesity Prevalence Among Adults With Hypertension in the United States, 2001 to 2023.

Background: Obesity is a factor contributing to the occurrence of hypertension and a risk factor for adverse outcomes in populations with hypertension. The changes in the prevalence of obesity in populations with hypertension remain unclear. Investigating the changes in the prevalence of obesity in populations with hypertension can provide information for the treatment and management of hypertension.

Predicting Antidepressant Treatment Response From Cortical Structure on MRI: A Mega-Analysis From the ENIGMA-MDD Working Group.

Accurately predicting individual antidepressant treatment response could expedite the lengthy trial-and-error process of finding an effective treatment for major depressive disorder (MDD). We tested and compared machine learning-based methods that predict individual-level pharmacotherapeutic treatment response using cortical morphometry from multisite longitudinal cohorts. We conducted an international analysis of pooled data from six sites of the ENIGMA-MDD consortium (n = 262 MDD patients; age = 36.

From PD-1/PD-L1 to tertiary lymphoid structures: Paving the way for precision immunotherapy in cholangiocarcinoma treatment.

Cholangiocarcinoma (CCA) is a highly malignant hepatobiliary tumor characterized by limited treatment options and poor prognosis. The recent rise of immunotherapy has significantly influenced research in this field. This study presents a bibliometric analysis of 416 articles retrieved from the WOSCC, Wan fang Data, CNKI and VIP databases, spanning contributions from 32 countries, 589 institutions and 3,200 authors.

Advances in cross-coupling and oxidative coupling reactions of NH-sulfoximines - a review.

Due to the special structure and physicochemical properties of sulfoximines, research on sulfoximines has achieved great progress in recent decades, especially in chemical and medicinal fields. This review highlights recent advancements in the N-functionalization of NH-sulfoximines, focusing on classical cross-coupling reactions with electrophilic agents and oxidative coupling reactions with extensive organic compounds, including specific (hetero)arenes, alkenes (1,4-naphthoquinones), alkanes (cyclohexanes), nucleophiles (thiols, disulfides, sulfinates, diarylphosphine oxides), organyl boronic acids, and arylhydrazines. Transition metal-catalyzed, metal-free, electrochemical and radical oxidative coupling reactions are discussed.

A Slimming/Excavating Strategy for Enhanced Intratumoral Penetration of Acid-Disassemblable NO-Releasing Nanomedicines.

Poor tumor penetration is the major predicament of nanomedicines that limits their anticancer efficacy. The dense extracellular matrix (ECM) in the tumor is one of the major barriers against the deep penetration of nanomedicines. In this work, a slimming/excavating strategy is proposed for enhanced intratumoral penetration based on an acid-disassemblable nanomicelles-assembled nanomedicine and the NO-mediated degradation of ECM.

Assessment of the application of the FA280-a fully automated fecal analyzer for diagnosing clonorchiasis: a mixed-method study.

Background: Clonorchiasis is an important foodborne parasitic disease in China caused by Clonorchis sinensis. Accurate and rapid diagnosis of this disease is vital for treatment and control. Traditional fecal examination methods, such as the Kato-Katz (KK) method, are labor-intensive, time-consuming, and have limited acceptance.

Future atherosclerotic cardiovascular disease in systemic lupus erythematosus based on CSTAR (XXVIII): the effect of different antiphospholipid antibodies isotypes.

Background: Patients with systemic lupus erythematosus (SLE) suffered from an increasing risk of cardiovascular diseases. In this multi-center prospective study, we aimed to determine the association between antiphospholipid antibodies (aPLs) and future atherosclerotic cardiovascular disease (ASCVD) in SLE.

Methods: In total, 1573 SLE patients were recruited based on the Chinese SLE Treatment and Research group (CSTAR) registry.

Systematic and comprehensive insights into HIF-1 stabilization under normoxic conditions: implications for cellular adaptation and therapeutic strategies in cancer.

Hypoxia-inducible factors (HIFs) are essential transcription factors that orchestrate cellular responses to oxygen deprivation. HIF-1α, as an unstable subunit of HIF-1, is usually hydroxylated by prolyl hydroxylase domain enzymes under normoxic conditions, leading to ubiquitination and proteasomal degradation, thereby keeping low levels. Instead of hypoxia, sometimes even in normoxia, HIF-1α translocates into the nucleus, dimerizes with HIF-1β to generate HIF-1, and then activates genes involved in adaptive responses such as angiogenesis, metabolic reprogramming, and cellular survival, which presents new challenges and insights into its role in cellular processes.

Acidosis induces autophagic cell death through ASIC1-mediated Akt/mTOR signaling in HT22 neurons.

Although it has been confirmed that acid-sensing ion channel 1 (ASIC1) plays a critical role in acidosis-induced neuronal injury and death, its underlying mechanisms remain largely unclear. In the present study, we investigated the involvement of ASIC1 in acidosis-induced neuronal death and its underlying mechanisms in HT22 neurons. The neurons were cultured in acidic medium to mimic extracellular acidosis.

Molecular recognition of the promoter DNA signature sequence by Hms1p.

Transcriptional regulation of sterol biosynthetic genes is mediated by conserved sterol-regulatory element binding proteins (SREBPs) in human pathogenic fungi, however, its homolog in S. cerevisiae regulate filamentous growth during stress conditions. These pseudohyphal growths might be associated with the expression of MEP2 gene in response to ammonium limitation.

Gibberellin promotes xylem expansion and cell lignification by regulating sugar accumulation and the expression of JcMYB43 and JcMYB63 in the woody plant Jatropha curcas.

Gibberellins (GAs) are a group of diterpene plant hormones that regulate various plant developmental processes, including wood formation. Nevertheless, the regulatory pattern and the downstream targets of GA in the regulation of xylem expansion and cell lignification in woody plants remain unclear. In transgenic Jatropha curcas with significantly increased or decreased bioactive GA content via separate overexpression of JcGA20ox1 or JcGA2ox6, comparative transcriptomic, metabolomic and physiological investigations were conducted on the young stems.

Engineering an Mn(II)-oxidizing Pseudomonas whole-cell catalyst chassis to efficiently biosynthesize 2,5-furandicarboxylic acid from hydroxymethylfurfural.

2,5-Furandicarboxylic acid (FDCA) is a high-value chemical extensively used in the production of bio-based polymers, but bioconversion of furan derivatives like 5-hydroxymethylfurfural (HMF) into FDCA remains challenging owing to substrate cytotoxicity. Here, we engineered an Mn(II)-oxidizing Pseudomonas sp. MB04B for efficient FDCA biosynthesis from HMF.