Development of an improved technique for the perfusion of the isolated caudal lobe of sheep liver.

The study was designed to develop an improved technique for perfusing the isolated caudal lobe of sheep liver. Twenty caudal lobes were perfused for 3-4 h, in a non-recirculating mode, with Krebs-Henseleit bicarbonate buffer. The perfusion system was designed to give a constant flow.

Comparative effects of adrenaline and noradrenaline on the synthesis and secretion of soluble protein by isolated hepatocytes.

1. Adrenaline (A) supplementation of the incubation medium of monolayer cultures of hepatocytes at 0.1, 0.

The effect of hydrocortisone and albumin on the synthesis and secretion of soluble proteins by isolated hepatocytes.

Monolayer cultures of hepatocytes were prepared. Supplementation of the incubation medium with bovine serum albumin increasing from 150 mg% to 6% resulted in an increase in retained and total newly synthesised proteins. No clear effects on secretion of soluble proteins were observed.

Effects of anti-albumin sera on the synthesis and secretion of soluble proteins by monolayers of hepatocytes.

Hepatocytes were isolated from male Sprague-Dawley rats and monolayer cultures prepared. The effects of supplementation of the incubation medium with homologous and heterologous antisera directed against albumin on the synthesis and secretion of soluble proteins were ascertained. Supplementation of the medium with antisera generally resulted in a time related decrease in the synthesis and secretion of soluble proteins.

Blood composition in culled elephants and buffaloes.

Blood composition of succinyldicholine culled elephants and buffaloes was compared with that of undisturbed animals shot in the brain. The results show statistically significant differences in a number of variables including plasma ACTH and cortisol concentrations. The observed changes are attributed to stress induced by a combination of herding and darting with succinyldicholine and asphyxia.

Protein and fat metabolism of hepatocytes from hyperosmolar non-ketotic diabetic rats.

The secretion of newly synthesized proteins and urea, triacylglycerol and beta-hydroxybutyrate by hepatocytes in cultures from normal and hyperosmolar non-ketotic diabetic rats has been compared. Secretion of protein and of urea was very similar in the two preparations. Secretion of protein was enhanced by addition of insulin to the culture medium, that of urea was decreased.

The influence of hyperosmolar non-ketotic diabetes on protein synthesis in rat tissues.

We have compared the effects of acute ketotic diabetes on the capacity of isolated ribosomes to carry out protein synthesis with the effects of the non-ketotic but hyperosmolar state developed in rats as a model of the human condition by the procedure described by Joffe et al. These workers concluded that the hyperosmolar non-ketotic condition was consistent with the liver receiving adequate insulin but with the periphery being insulin-deficient. In support of this hypothesis we find that there is no impairment of hepatic protein synthesis as judged by the activity of isolated ribosomes, nor of the proportion of ribosomes in polysomes in the hyperosmolar non-ketotic condition, this being in contrast with the ketotic state, where protein synthesis and polysome levels are markedly reduced.

Urea cycle enzymes and glutamate dehydrogenase in Xenopus laevis and Bufo viridis adapted to high salinity.

Xenopus laevis was adapted stepwise to 600 m osmolar sodium chloride. After adaptation, the level of argininosuccinate lyase was raised 9-fold, carbamoylphosphate synthetase 6-fold, and ornithine carbamoyltransferase and arginase 3-fold. Liver glutamate dehydrogenase was also raised 5-fold; kidney glutamate dehydrogenase was unchanged.