Investigation of conceptus stimulated gene expression in buffalo peripheral blood mononuclear cells as potential diagnostic markers of early pregnancy.

Exploration of novel strategies for early pregnancy diagnosis is pivotal in enhancing the reproductive potential and monetary gains from dairy herds. In buffalo, the trophectoderm cells of the elongating conceptus secrete interferon-tau that stimulates the transcription of various genes in peripheral blood mononuclear cells (PBMC) during the peri-implantation period. We explored the differential expression of classical () and novel ( and ) early pregnancy markers in PBMC of buffaloes during various stages of pregnancy.

Evaluation of adenosine deaminase activity in serum of cattle and buffaloes in the diagnosis of bovine tuberculosis.

Background And Aim: Bovine tuberculosis (bTB) is a chronic bacterial disease of cattle caused by . bTB causes severe economic losses resulting from livestock deaths, chronic disease, and trade restrictions. Determination of serum levels of adenosine deaminase (ADA), an enzyme produced by monocytes/macrophages and lymphocytes, has been used in the diagnosis of human TB.

ISMap element targeted nested polymerase chain in the detection of subsp. in fecal samples of cattle and buffaloes.

Background And Aim: Johne's disease is chronic granulomatous enteritis which affects ruminants. There are many diagnostic approaches for the detection of subsp. (MAP) of which molecular detection methods using various elements are less time consuming and more accurate.