Stress-induced GHS-R1a expression in medial prefrontal cortical neurons promotes vulnerability to anxiety in mice.

The neural basis of anxiety is unclear, which hinders the treatment of anxiety disorders. Here, we found that αCaMKII neurons in the medial prefrontal cortex (mPFC) responded to stressors with increased activity both under physiological conditions and after repeated restraint stress (RRS) in mice. Chemogenetic activation of mPFC neurons ameliorated stress-induced anxiety.

Mulberry Leaves-Derived Carbon Dots for Photodynamic Treatment of Methicillin-Resistant -Infected Wounds via Metabolic Perturbation.

Antibiotic-resistant pathogens pose a significant global public health challenge, particularly in refractory infections associated with biofilms. The urgent development of innovative, safe, and therapeutically adaptive strategies to combat these resistant biofilms is essential. We present a novel, precise, and controllable photodynamic antibacterial carbon dot (B-M-CD) inspired by the natural antibacterial properties of the mulberry leaf and the bacterial targeting function of boric acid.

Complex associations among modifiable determinants of circadian syndrome among employed people in southwestern China.

Background: Circadian syndrome (CircS) may be closely linked to lifestyle, psychological, and occupational factors, but evidence is lacking. This study aimed to explore complex associations between lifestyle, psychological, and occupational factors and CircS among employed people in southwestern China.

Methods: In this study, network analysis was used to identify complex associations between lifestyle, psychological, and occupational factors and CircS in employed people from the Chinese Cohort of Working Adults (CCWA).

Sanguinarine induces the unfolding of Staphylococcus aureus-derived FtsZ and reduces the conformational flexibility necessary for assembly.

Methicillin-resistant Staphylococcus aureus (MRSA) represents a serious global health challenge due to its resistance to multiple antibiotics, especially β-lactams, which limits treatment options and results in high morbidity and mortality rates. Thus, there is an urgent need for effective antibacterial agents and a deeper understanding of their mechanisms of action to combat the increasingly severe threat of bacterial resistance. In this study we have performed microbiological, molecular biology experiments and molecular dynamics simulations to investigate the antimicrobial efficacy of the sanguinarine (SAN) against MRSA and its conformational modulation of the Filamentous temperature-sensitive mutant Z (FtsZ).

(Apo)Lipoprotein Profiling with Multi-Omics Analysis Identified Medium-HDL-Targeting PSRC1 with Therapeutic Potential for Coronary Artery Disease.

Identification of (apo)lipoprotein subclasses causally underpinning atherosclerosis may lead to identification of novel drug targets for treatment of atherosclerotic cardiovascular disease (ASCVD). In this study, observational and genetic associations between (apo)lipoprotein profile and carotid intima-media thickness-assessed atherosclerosis, and risks of coronary artery disease (CAD) and ischemic stroke (IS) are assessed, using data from the UK Biobank study, with further exploration of potential drug target for these two ASCVD subtypes through multi-omics analysis integrating genetic, transcriptomic, and proteomic data. Cholesteryl ester content in medium high-density lipoprotein causally protective of atherosclerosis is identified, plus a target gene, PSRC1, with therapeutic potential for CAD, but not IS, supported by consistent evidence from multi-omics layers of data, which also reveals that such therapeutic potential may be through downregulation of circulating proteins including TRP1, GRNs, and Pla2g12b, and upregulation of Neo1.

Mediating effect of illness perception between self-care ability and health-promoting behaviors among patients with stable coronary artery disease.

Background: The interaction between illness perception, self-care ability, and health-promoting behaviors (HPB) in stable coronary artery disease (SCAD) patients remains uncertain. We conducted a cross-sectional survey to explore the correlation between self-care ability, illness perception, and HPB among patients with SCAD, as well as the potential mediating role of illness perception between self-care ability and HPB.

Methods: A cross-sectional study was carried out among 184 inpatients with SCAD in Hefei, China, from December 2022 to March 2023.

NONHSAT141192.2 Facilitates the Stemness and Radioresistance of Glioma Stem Cells via the Regulation of PIK3R3 and SOX2.

Background: Glioma stem cells (GSCs) contribute to the initiation, recurrence, metastasis, and drug resistance of glioblastoma multiforme (GBM). Long noncoding RNAs (lncRNAs) are critical modulators in the development and progression of GBM; however, specific lncRNAs related to GSCs remain largely unexplored. This study aims to identify dysregulated lncRNAs in GSCs, unravel their contributions to GBM progression, and propose new targets for diagnosis and treatment.

Single-Cell Transcriptome Decoding Umbilical Cord-Derived Mesenchymal Stem Cell Heterogeneity Reveals a Unique IL1R1PDGFRA Ultroser-G-MSC With Osteogenesis and Chondrogenesis Signatures.

The heterogeneity of human umbilical cord mesenchymal stem cells (hUC-MSCs) is culturing-dependent, resulting in functional non-uniformness. To achieve the best clinical benefit, a comprehensive understanding of the origin of the heterogeneity in different culture systems can identify functional subgroups to direct the precise application of hUC-MSCs. Here, we create a single-cell transcriptome atlas of hUC-MSC in different culture systems for the identification of a subgroup of Ultroser-G-MSCs with high osteogenic and chondrogenic potentials featured by high expressions of IL1R1 and PDGFRA.

Anti-disturbance control of permanent magnet eddy-current coupler speed control system based on disturbance interval observer.

For the permanent magnet eddy-current coupler speed control system with external disturbances, consideration is given to achieving anti-disturbance objectives through disturbance compensation. Utilizing partial information of disturbance dynamics, it is convenient to design a disturbance interval observer (DIO), generating upper and lower bounds covering each element of the external disturbances, where the positive system theory ensures non-negativity of errors. Then, based on the DIO, disturbance reconstruction is performed for feedforward compensation, while feedback control is employed for tracking and stabilization, eliminating control chattering generated by the sliding mode.

SNR enhanced OAM mode division multiplexing with in-band noise modulation based on cascade delta-sigma modulation.

Due to mode coupling, a high signal-to-noise ratio (SNR) is required in orbital angular momentum (OAM) modular division multiplexing (MDM) systems to improve transmission performance. In this paper, a cascade delta-sigma modulation (CDSM) scheme is proposed for OAM-MDM intensity modulation and direct detection (IM/DD) transmission. Different from the traditional DSM (TDSM) scheme, the scheme is divided into signal modulation and in-band noise modulation, in which the in-band noise modulation is used to further decrease the quantization noise generated in the signal modulation.

Longitudinal Assessment of Pulmonary Involvement and Prognosis in Different Subtypes of COVID-19 Patients After One Year Using Low-Dose CT: A Prospective Observational Study.

Rationale And Objectives: Severe COVID-19 typically results in pulmonary sequelae. However, current research lacks clarity on the differences in these sequelae among various clinical subtypes. This study aimed to evaluate the changing lung imaging features and predictive factors in patients with COVID-19 pneumonia in northern China over a 12-month follow-up period after the relaxation of COVID-19 restrictions in 2022.

Odorant production surges induced by exogenous oxidative stress: An overlooked risk in PAA-based moderate preoxidation for algal-laden water.

Moderate preoxidation is feasible for odor-producing algae treatments, usually requiring trade-offs in algal removal and integrity maintenance. However, dosing oxidants may cause internal oxidative homeostasis imbalances and secondary odorous metabolite responses, adding new trade-offs for moderate treatments. Peracetic acid (PAA)/Fe processes are promising strategies in moderate treatments and thus were applied to examine how to achieve the following three trade-offs: good algal removal, no odorant increases and no releases.

Exploring the Adaptation Process of to High Temperatures Based on Changes in Intestinal Microbiota.

Global warming has led to rising water temperatures, posing a significant threat to fish survival. Understanding the mechanisms by which fish respond to and adapt to temperature variations is thus of considerable importance. This study employed high-throughput 16S rRNA gene sequencing and bioinformatics to investigate changes in the intestinal microbiota of the kaluga sturgeon () under four temperature conditions (19 °C, 25 °C, 28 °C, and 31 °C) and its relationship with adaptation to high-temperature stress.

Tripartite motif-containing 32 regulated by miR-6236-p5 inhibited silica-induced apoptosis of alveolar macrophages.

Apoptosis of alveolar macrophages (AMs) induced by silica is one of the crucial driving factors of silicosis inflammation and fibrosis. However, the mechanism of silica-induced AMs apoptosis remains unclear. In this study, transcriptome sequencing identified 11 differentially expressed (DE)-mRNAs enriched in the regulation of apoptotic signaling pathways in AMs treated with 250 μg/mL silica for 24 h, of which tripartite motif-containing 32 (Trim32) was the most significant and down-regulated.

Early detection of psoriatic arthritis in patients with psoriasis: construction of a multifactorial prediction model.

Psoriatic arthritis (PsA) affects approximately one in five individuals with psoriasis. Early identification of patients with psoriasis at risk of developing PsA is crucial to prevent poor prognosis. We established a derivation cohort comprising 1,661 patients with psoriasis from 49 hospitals.

Early diagnosis of type 2 diabetes mellitus in patients with psoriasis: Construction of a multifactorial diagnostic model.

Background: Type 2 diabetes mellitus (T2DM) is a significant comorbidity of psoriasis that exerts a long-term detrimental effect on patients. Early T2DM detection in psoriasis patients is essential for timely medical intervention and the prevention of further complications.

Objective: To develop a diagnostic model and introduce a web-based calculator for assessing the risk of T2DM in patients with psoriasis.

Unveiling relationships of human instinctive behaviors and blood pressure in Chinese adults: A network analysis.

Objectives: Human behaviors and blood pressure (BP) are closely intertwined, affecting health. However, few studies have demonstrated their complex relationships. Using network analysis, we aimed to untangle the intricate and potential causal relationships between BP and human instinctive behaviors, identifying potential action points for hypertension prevention and control.

Kinetic Profiling of Oxidoreductase-Mimicking Nanozymes: Impact of Multiple Activities, Chemical Transformations, and Colloidal Stability.

In contrast to homogeneous enzyme catalysis, nanozymes are nanosized heterogeneous catalysts that perform reactions on a rigid surface. This fundamental difference between enzymes and nanozymes is often overlooked in kinetic studies and practical applications. In this article, using 14 nanozymes of various compositions (core@shell, metal-organic frameworks, metal, and metal oxide nanoparticles), we systematically demonstrate that nontypical features of nanozymes, such as multiple catalytic activities, chemical transformations, and aggregation, need to be considered in nanozyme catalysis.

Targeted metabolomics identified novel metabolites, predominantly phosphatidylcholines and docosahexaenoic acid-containing lipids, predictive of incident chronic kidney disease in middle-to-elderly-aged Chinese adults.

Background: Evidence is limited regarding the association of circulating metabolites with decline of kidney function, letting alone their value in prediction of development of chronic kidney disease (CKD).

Methods: This study included 3802 participants aged 64.1 ± 7.

Strategy for Accurate Detection of Six Tropane Alkaloids in Honey Using Lateral Flow Immunosensors.

Honey, a widely consumed food, is susceptible to contamination by various toxic substances during production. Tropane alkaloids, with their potent neurotoxicity, are frequently found in honey. Hence, there is an acute need for rapid and effective detection methods to monitor these alkaloids.

Nanozyme-Enzyme Cascade Reaction-Enhanced Ratiometric Fluorescence Immunosensing Platform for Sensitive and Accurate Detection of Ractopamine.

As the most widely used immunoassay, enzyme-linked immunosorbent assay (ELISA) relies on perishable enzymes and usually provides poor sensitivity and stability, limiting its application in detecting trace analytes in harsh environments. Herein, a new ratiometric fluorescence (RF) sensing platform enhanced by a nanozyme-enzyme cascade reaction composed of MnO nanosheets (MnO NSs) and alkaline phosphatase (ALP) was proposed. In this RF platform, the versatile MnO NSs worked as a robust oxidase-like nanozyme to catalyze nonfluorescent Amplex Red (AR) into fluorescent resorufin and as a quencher to quench the fluorescence of carbon dots (CDs).