Automated Immunoassay Performed on a 3D Microfluidic Paper-Based Device for Malaria Detection by Ambient Mass Spectrometry.

Microfluidic paper-based analytical devices (μPADs) are emerging as a prominent platform for disease detection, specifically in developing countries. This paper device offer simplicity and affordability not typically seen in centralized laboratory settings. However, detection limits in μPADs are inadequate and often require test results to be read within a specific time interval to ensure accuracy.

Enhanced thread spray mass spectrometry: a general method for direct pesticide analysis in various complex matrices.

Determination of pesticide residues in a wide variety of matrices is an ongoing challenge due to low concentration and substantial amounts of interfering endogenous compounds that can be coextracted with the analytes. Herein, we describe the use of cellulose thread both as a suitable sampling medium for various matrices and as a direct analysis platform through an improved thread spray mass spectrometry (MS) approach. Enhanced extraction and the subsequent generation of tiny nanodroplets, after the application of DC potential to the wet thread, enabled ultra-sensitive detection of pesticides without prior sample treatment.

Direct differentiation of whole blood for forensic serology analysis by thread spray mass spectrometry.

Direct analysis of whole blood on bloodstained textiles is achieved with thread spray mass spectrometry (MS). This capability satisfies investigators' first priority in crime scene investigations, which is determining if a stain is blood. This thread spray method explores the use of evidentiary fabric threads for rapid determination of hemoglobin directly from whole blood within textiles without prior extraction steps.

Applications of Mass Spectrometry for Clinical Diagnostics: The Influence of Turnaround Time.

This critical review discusses how the need for reduced clinical turnaround times has influenced chemical instrumentation. We focus on the development of modern mass spectrometry (MS) and its application in clinical diagnosis. With increased functionality that takes advantage of novel front-end modifications and computational capabilities, MS can now be used for non-traditional clinical analyses, including applications in clinical microbiology for bacteria differentiation and in surgical operation rooms.

Microsampling with cotton thread: Storage and ultra-sensitive analysis by thread spray mass Spectrometry.

Storage and quantitative analysis of small volumes of biofluids are challenging, especially when low concentrations of analytes are to be detected in the presence of complex matrices. In this study, we describe an integrated thread-based approach for stabilizing small blood volumes in the dry-state at room temperature, while also offering direct analysis capabilities via thread spray mass spectrometry. The analytical merits of this novel microsampling platform was demonstrated via the direct analysis of diazepam and cocaine in dried blood samples stored for 42 days.

Thread spray mass spectrometry for direct analysis of capsaicinoids in pepper products.

Thread spray ambient ionization uses single threads as a medium for sampling and ionization. This approach was demonstrated through the detection of various capsaicinoids from the interior of pepper fruits without destruction of the sample. Pepper residues present on the thread were analyzed by the application of DC voltage and solvent to cause field-induced charged droplet generation.