Publications by authors named "Sierra A Sterling"
Article Synopsis
- - The deamination of adenosine to inosine is a key modification in nucleic acids that changes the identity of the base, complicating traditional detection methods like sequencing and PCR that are often slow and expensive.
- - Researchers have developed fluorescent "turn-on" FIT-PNA probes that bind specifically to inosine in nucleic acids, using various fluorogenic dyes to facilitate real-time detection of A-to-I editing events.
- - The study found that while incorporating these dyes decreases duplex stability, they still effectively bind to their targets; optimal detection yielded an 11-fold fluorescence increase with a low detection limit of 30 pM, highlighting the potential for these probes in biological applications.
Aptamers are widely used in small molecule detection applications due to their specificity, stability, and cost effectiveness. One key challenge in utilizing aptamers in sensors is matching the binding affinity of the aptamer to the desired concentration range for analyte detection. The most common methods for modulating affinity have inherent limitations, such as the likelihood of drastic changes in aptamer folding.
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