Propionic Acidemia, Methylmalonic Acidemia, and Cobalamin C Deficiency: Comparison of Untargeted Metabolomic Profiles.

Methylmalonic acidemia (MMA), propionic acidemia (PA), and cobalamin C deficiency (cblC) share a defect in propionic acid metabolism. In addition, cblC is also involved in the process of homocysteine remethylation. These three diseases produce various phenotypes and complex downstream metabolic effects.

Effect of a hyaluronic acid-based mesotherapeutic injectable on the gene expression of CLOCK and Klotho proteins, and environmentally induced oxidative stress in human skin cells.

Objective: Normal circadian rhythms are essential to the repair mechanisms of oxidative stress implicated in skin aging. Given reports that hyaluronic acid (HA) homeostasis exhibits a different profile in chronological skin aging, as compared to environmental or extrinsic aging, an improved understanding of the way HA interacts with its surroundings, and the impact of HA injectables in replacing lost HA and encouraging rejuvenation, is of key benefit to skin aging treatments. The objectives of these current studies were twofold.

Signaling Molecules of Human Skin Cells as the Targets for Injection Cosmetology.

Introduction: Skin aging is a natural process that cannot be stopped. However, there are many ways to help attenuate premature aging of the skin and reduce the signs that have already appeared. One of them is the subcutaneous administration of preparations containing a combination of hyaluronic acid, active amino acids, and peptides providing an anti-aging clinical effect.

Ethylmalonic encephalopathy and liver transplantation: long-term outcome of the first treated patient.

Background: Ethylmalonic encephalopathy (EE) is a severe intoxication-type metabolic disorder with multisystem clinical features and leading to early death. In 2014, based on the promising results obtained by liver-targeted gene therapy in Ethe1 mouse model, we successfully attempted liver transplantation in a 9-month-old EE girl. Here we report her long-term follow-up, lasting over 6 years, with a comprehensive evaluation of clinical, instrumental and biochemical assessments.

Recombination of Correlations between Bioelements in the Liver and Lungs during Modeling of Diet-Induced Obesity.

In rats with obesity induced by high fat ration, the concentrations of K, Ca, Mn, Fe, Cu, Zn, Se, Br, Rb, and Sr were measured in the liver and lungs using synchrotron X-ray fluorescence analysis. Recombinations of inter-element correlations in the liver and lungs of rats receiving or not receiving additional Zn were observed under fasting conditions and after eating lard. Sr and Ca made the most pronounced contribution in the redistribution of correlations in the liver of obese rats.

Content of bioelements in the lungs and liver in rats with alimentary obesity.

The synchrotron radiation X-ray fluorescence technique (SRXRF) was applied to the determination of K, Ca, Mn, Fe, Cu, Zn, Se, Br, Rb, and Sr concentrations in the liver and lungs in Wistar rats. The animals in the experiment included (1) healthy rats, (2) rats with alimentary obesity (AO), and (3) rats with alimentary obesity that were being given zinc sulphate with water for a long time (АО+Zn). Each group was divided into two subgroups.

Effect of zinc on the content of chemical elements in the lung tissue during obesity in the experiment.

We found no deviations from normalcy in the content of chemical elements (K, Ca, Mn, Fe, Cu, Zn, Se, Br, Rb, and Sr) in the lungs of rats with mild alimentary obesity, but revealed redistribution of correlations between the elements indicating impaired metabolism in this organ. Zinc supplementation had no effect on the body weight and content of chemical elements (including zinc) in the lung tissue in rats fed high fat diet, but led to significant changes in the correlations between the elements. Bromine, rubidium, and strontium are actively involved in interelement interactions in the lung tissue.

[Behavioral effects during the local activation and blockade of serotonin and dopamine receptors in the frontal cortex in cats in the model with a choice of reinforcements of different value].

In the model with a choice of reinforcements of different value animals were able to "impulsive" and "self-controlled" behavior with an equal probability. Five adult cats were tested. A local application of agonists of 5-HT(1A) and 5-HT(2A/C) receptors (8-OH-DPAT, DOI) in the frontal cortex have resulted in a significant decrease of the "impulsive" reactions and an increase of omissions.

Measurement of X-ray mass attenuation coefficients in biological and geological samples in the energy range of 7-12keV.

Information about X-ray mass attenuation coefficients in different materials is necessary for accurate X-ray fluorescent analysis. The X-ray mass attenuation coefficients for energy of 7-12keV were measured in biological (Mussel and Oyster tissues, blood, hair, liver, and Cabbage leaves) and geological (Baikal sludge, soil, and Alaskite granite) samples. The measurements were carried out at the EXAFS Station of Siberian Synchrotron Radiation Center (VEPP-3).

[Interelement correlations in the lungs and the liver in alimentary obesity].

Using a high-fat diet in experiment on rats received model alimentary obesity (AO). Adding zinc to the diet did not affect the severity of the AO. The concentration of K, Ca, Mn, Fe, Cu, Zn, Se, Br, Rb, Sr in the liver and lungs of rats with obesity in both groups did not differ from that of healthy animals.

Effects of zinc on the content of chemical elements in the liver of rats during early stages of obesity.

In rats with slight alimentary obesity, the content of chemical elements in the liver did not differ from normal, but the correlations between these elements were changed, which attested to metabolic disorders. Additional zinc dose administered to rats receiving lipid-rich rations did not affect animal body weights and content of chemical elements (including zinc) in the liver, but corrected correlations between the elements. Active contribution of strontium, bromine, and rubidium to interactions between the elements in the liver necessitates studies of their role in biological processes, specifically, in initiation and development of obesity.

Micronutrients and macronutrients in the liver in experimental alimentary obesity.

Strong and very strong positive correlations differing from those in the control were found between chemical elements in the liver of rats with experimental alimentary obesity under conditions of physiological hunger and soon after fatty food intake. These findings attest to sustained metabolism disturbances in obesity and formation of a pathological system. Considering the appearance of strong and very strong correlations of Br and Rb in alimentary obesity, more attention should be paid to the investigation of the role of these poorly studied elements in lipid metabolism.

Changes in the elemental content of rat heart as a result of the fixation in formalin analyzed by synchrotron radiation X-ray fluorescent analysis.

The aim of the study was to investigate possible changes in the elemental content of small biological tissue samples (with low weight) during the short period of fixation in formalin. Therefore, the effect of the fixation time (4 h to 6 days) was studied on the elemental content of the tissue. Synchrotron radiation X-ray fluorescent analysis (SRXRF) technique was used for the determination of the elemental concentrations.