Publications by authors named "Siddhardha B"

Background: Rhodopsin is a seven-transmembrane protein covalently linked with retinal chromophore that absorbs photons for energy conversion and intracellular signaling in eukaryotes, bacteria, and archaea. Haloarchaeal rhodopsins are Type-I microbial rhodopsin that elicits various light-driven functions like proton pumping, chloride pumping and Phototaxis behaviour. The industrial application of Ion-pumping Haloarchaeal rhodopsins is limited by the lack of full-length rhodopsin sequence-based classifications, which play an important role in Ion-pumping activity.

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Mangroves are situated in convergence zones between fresh and marine water and are prone to pollution and deforestation. This study explored the microbiome structure, function and antibiotic resistome of Indian Sundarban. The taxonomic Chao1 estimated diversity was highest in uninhabited Kalash (1204.

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The biosynthesis of nanoparticles (NPs) has gained an overwhelming interest due to their biological applications. However, NPs synthesis by pigmented extreme halophiles remains underexplored. The NPs synthesis using pigmented halophiles is inexpensive and less toxic than other processes.

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Signal dependent microbial communication in Pseudomonas aeruginosa PAO1 is a typical phenomenon mediated by acyl homo-serine lactone molecules that helps in developing biofilm and enhance antibiotic resistance. Microbial sources provide insight to the hidden treasure of secondary metabolites, and these structurally diversified chemical motifs can be used as antimicrobial and anti-infective agents. In the present study, endophytic fungus, Colletotrichum gloeosporioides HM3 isolated from Carica papaya leaves was explored for anti-infective potential against P.

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Article Synopsis
  • Haloarchaea are microorganisms found in highly saline environments, which may contain valuable biomolecules like carotenoids and proteins, yet their diversity in Indian saline areas is under-researched.
  • A study identified 12 novel haloarchaeal strains from a solar saltern in Kottakuppam, Tamil Nadu, revealing that most produce carotenoids, primarily bacterioruberin and lycopene, and classifying them into yellow and orange based on color differences.
  • Whole-genome sequencing of select strains led to the identification of unique genes related to biological functions, and successful cloning of functional bacteriorhodopsin, which plays a role in ATP production by using light energy.
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The persistence of multidrug resistance among microorganisms has directed a mandate towards a hunt for the development of alternative therapeutic modalities. In this context, antimicrobial photodynamic therapy (aPDT) is sprouted as a novel strategy to mitigate biofilms and planktonic cells of pathogens. Nanoparticles (NPs) are reported with unique intrinsic and antimicrobial properties.

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Microbial communities perform crucial biogeochemical cycles in distinct ecosystems. Halophilic microbial communities are enriched in the saline areas. Hence, haloarchaea have been primarily studied in salterns and marine biosystems with the aim to harness haloarcheal carotenoids biosynthesis.

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Endophytic fungi provide rich reservoir for novel antimicrobial compounds. An endophytic fungus, from plant identified as , was investigated for attenuating the quorum sensing mediated pathogenicity of PAO1. Crude extract of was found to reduce the production of redox-active pigments-pyocyanin and pyoverdine in PAO1 by 92.

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The application of nanotechnology in medicine is gaining popularity due to its ability to increase the bioavailability and biosorption of numerous drugs. Chrysin, a flavone constituent of is well-reported for its biological properties. However, its therapeutic potential has not been fully exploited due to its poor solubility and bioavailability.

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Quorum sensing (QS)-mediated infections cause severe diseases in human beings. The control of infectious diseases by inhibiting QS using antipathogenic drugs is a promising approach as antibiotics are proving inefficient in treating these diseases. Marine fungal ( PPR) extract was found to possess effective antipathogenic characteristics.

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Titanium dioxide nanoparticles (TiO-NPs) were synthesized using the aqueous leaf extract of Aloe barbadensis as a reducing and fabricating agent. The biosynthesis of the TiO-NPs was initially confirmed by UV-vis spectroscopy. Based on the HRTEM and FESEM analysis, the biosynthesized NPs were found to be polydispersed and predominantly spherical in shape, with an average size of ~20 nm.

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The bacterial cell communication also termed as Quorum sensing (QS) system was involved in the expression of several virulence traits during Pseudomonas infection. The attenuating of this bacterial cell communication system is an attractive approach for the management of bacterial infections without the complication of resistance development. In this respect, the marine environment has gained significant attention due to its biodiversity and as a source of novel bioactive compounds.

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Background: Rise in the number of healthcare associated or hospital acquired infections is a major problem affecting the global healthcare sector. We evaluated superior antibacterial and antibiofilm photodynamic therapy (aPDT) using malachite green encapsulated mesoporous silica nanoparticles (MG-MSN) against and , which are known to be major causative agents of nosocomial infections.

Methods: Malachite green (MG) was encapsulated on mesoporous silica nanoparticles (MSN).

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With the ever-increasing population and improvement in the healthcare system in the 21st century, the incidence of chronic microbial infections and associated health disorders has also increased at a striking pace. The ability of pathogenic microorganisms to form biofilm matrix aggravates the situation due to antibiotic resistance phenomenon resulting in resistance against conventional antibiotic therapy which has become a public health concern. The canonical Quorum Sensing (QS) signaling system hierarchically regulates the expression of an array of virulence phenotypes and controls the development of biofilm dynamics.

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Infections associated with medical devices that are caused by biofilms remain a considerable challenge for health care systems owing to their multidrug resistance patterns. Biofilms of and can result in life-threatening situations which are tough to eliminate by traditional methods. Antimicrobial photodynamic inactivation (aPDT) constitutes an alternative method of killing deadly pathogens and their biofilms using reactive oxygen species (ROS).

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In the present study, the antimicrobial and antibiofilm efficacy of toluidine blue (TB) encapsulated in mesoporous silica nanoparticles (MSN) was investigated against Pseudomonas aeruginosa and Staphylococcus aureus treated with antimicrobial photodynamic therapy (aPDT) using a red diode laser 670 nm wavelength, 97.65 J cm radiant exposure, 5 min). Physico-chemical techniques (UV-visible (UV-vis) absorption, photoluminescence emission, excitation, and FTIR) and high-resolution transmission electron microscopy (HR-TEM) were employed to characterize the conjugate of TB encapsulated in MSN (TB MSN).

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Article Synopsis
  • Plants have been used for centuries in traditional medicine due to their therapeutic potential for various health issues.
  • The study evaluated ethanolic leaf extracts of two plant species for their ability to scavenge free radicals, showing significant DPPH free radical scavenging activity.
  • In vivo studies with mutant yeast cells confirmed that these extracts effectively reduce reactive oxygen species (ROS), indicating their potential as antioxidants and future therapeutic applications.
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The ZnO thin films have been prepared by spin coating followed by annealing at different temperatures like 300°C, 350°C, 400°C, 450°C, 500°C & 550°C and ZnO nanoparticles have been used for photocatalytic and antibacterial applications. The morphological investigation and phase analysis of synthesized thin films well characterized by X-ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), Photoluminescence (PL), Transmission Electron Microscopy (TEM) and Raman studies. The luminescence peaks detected in the noticeable region between 350nm to 550nm for all synthesized nanosamples are associated to the existence of defects of oxygen sites.

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Ceria (CeO) is an exciting alternative noble metal catalyst, because it has ability to release and absorb oxygen in the redox system, and function as an oxygen buffer. In this study, heterostructured catalysts consisting of CeO/YO nanocomposites were successfully synthesized by hydrothermal method in the presence of sodium hydroxide as a reducing agent from cerium nitrate and yttrium nitrate as a precursor which was then evaluated for its photocatalytic activity in the degradation of Rhodamine B (RhB) synthetic dye. Scanning electron microscopy (SEM) imparts the surface morphology and size of the prepared sample.

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Background: From ancient times, plants and plant-derived products have been used as folkloric medicines for a variety of health disorders owing to their tremendous therapeutic potential. The present study aimed to determine the antioxidant efficacy of crude Acacia nilotica extract in the oxidative stress response system of Saccharomyces cerevisiae as a model organism.

Results: Acacia nilotica showed significant antioxidant activity, with IC values of 75.

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Due to the increased development of resistance of vectors against synthetic insecticides and chemical drugs, plant based insecticides serve as promising biocontrol agents for effective vector control. Green approach for the synthesis of nanoparticles has been attained using environmentally safe, non-toxic plant extracts. The present study was aimed to investigate the potent larvicidal activity of silver nanoparticles (AgNPs) produced by Derris trifoliata leaf extract in relation to the various concentrations of methanol and chloroform extracts for 24h against 3rd and 4th instar larvae of Aedes aegypti.

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