Emergence of Candida auris in intensive care units in Algeria.

Background: Currently, Candida auris is among the most serious emerging pathogens that can be associated with nosocomial infections and outbreaks in intensive care units. Clinicians must be able to identify and manage it quickly.

Objective: Here, we report for the first time in Algeria seven cases of C.

Prevalence and Antimicrobial Resistance of in Hospital Settings.

(1) Background: The purpose of this study was to determine the prevalence of clostridia strains in a hospital environment in Algeria and to evaluate their antimicrobial susceptibility to antibiotics and biocides. (2) Methods: Five hundred surface samples were collected from surfaces in the intensive care unit and surgical wards in the University Hospital of Tlemcen, Algeria. Bacterial identification was carried out using MALDI-TOF-MS, and then the minimum inhibitory concentrations (MICs) of various antimicrobial agents were determined by the E-test method.

Real-Time PCR Assay for Rapid and Simultaneous Detection of and Genes in Clinical Strains.

Here, we develop a robust and sensitive real-time PCR assay which allows the simultaneous detection of and genes using common primers. The system was designed using the Primer3 online software. The specificity of primers and probes was first checked by in silico PCR and by BlastN analysis.

High frequency and diversity of Vancomycin-resistant Enterococci (VRE) in Algerian healthcare settings.

The spread of vancomycin-resistant Enterococci (VRE) in Algerian hospital settings is poorly reported. Since the first report in 2006, few data have been available on the molecular mechanism of this resistance across the country. In this study, we investigate the frequency and antibiotic resistance mechanisms of Enterococci strains isolated from hospitalised patients in the Tlemcen university hospital.

miRNA-23b as a biomarker of culture-positive neonatal sepsis.

Background: Neonatal sepsis remains an important cause of morbidity and mortality. The ability to quickly and accurately diagnose neonatal sepsis based on clinical assessments and laboratory blood tests remains difficult, where haemoculture is the gold standard for detecting bacterial sepsis in blood culture. It is also very difficult to study because neonatal samples are lacking.

Biocide tolerance and antibiotic resistance of Enterobacter spp. isolated from an Algerian hospital environment.

Objectives: In this study, 77 Enterobacter spp. isolates from a collection of 175 Gram-negative bacilli isolated from Tlemcen University Hospital Center (North-West of Algeria) were tested for antibiotic resistance, biocide tolerance and genetic determinants of antimicrobial resistance.

Methods: The isolates were identified by 16S rDNA gene sequencing.