Publications by authors named "Sibulelo Vilakazi"

The design of electrochemical immunosensors for the detection of measles-specific antibodies is reported. The measles-antigen modified surface was used as an antibody capture surface. The detection of measles-specific IgG antibodies was accomplished using the voltammetric method and horse-radish peroxidase (HRP) labeled secondary antibody (anti-IgG) as a detecting antibody.

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Article Synopsis
  • - The study focuses on detecting measles-specific IgG antibodies using electrochemical impedimetric immunosensors, achieving optimal electrode conditions after 40 minutes.
  • - Surface roughness, measured by AFM, increased with each step of immobilization or antigen-antibody reaction, confirming successful surface modifications and interactions.
  • - Human serum and new-born calf serum were tested to simulate real-world samples, showing a correlation between antibody concentrations and charge-transfer resistance, highlighting the potential of impedance methods for label-free detection of measles antibodies.
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Gold nanoparticles (AuNPs) in aqueous 0.10 M HCl are shown to be electroactive at oxidising potentials greater than 1.0 V (vs.

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The pre-grafted screen-printed gold electrode modified with phenyl-amino monolayer was investigated for covalent immobilization of phenyl-amine functionalized single-walled carbon nanotubes (PA-SWCNT) and metal tetra-amino phthalocyanine (MTAPc) using Schiff-base reactions with benzene-1,4-dicarbaldehyde (BDCA) as cross-linker. The PA-SWCNT and MTAPc modified electrodes were applied as hybrids for electrochemical sensing of H(2)O(2). The step-by-step fabrication of the electrode was followed using electrochemistry, impedance spectroscopy, scanning electron microscopy and Raman spectroscopy and all these techniques confirmed the fabrication and the immobilization of PA-SWCNT, MnTAPc and CoTAPc onto gold surfaces.

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Metallophthalocyanine (MPc) and metalloporphyrin complexes as well as some metalloproteins including myoglobin, hemoglobin, cyanocobalamin and cytochrome c catalyse the detection of nitric oxide (NO). The catalytic process often involve changes in the oxidation state of the catalyst. These complexes catalyse both the reduction and oxidation of NO.

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