A CRISPR/Cas-Based Method for Precise DNA Integration in Xenopus laevis Oocytes Followed by Intracytoplasmic Sperm Injection (ICSI) Fertilization.

Xenopus has long had a reputation for being a powerful model organism for use in developmental cell and biochemistry research. With the advent of gene-editing technologies, and the full genome sequencing of Xenopus genomes revealing the extent of the genetic conservation between Xenopus and humans, Xenopus has the potential to become an ideal model for human genetic disease. However, the inability to produce non-mosaic, precise DNA insertions through homology directed repair has limited the strength of Xenopus this field.

Confirming Antibody Specificity in .

Verifying that a new antibody recognizes its target can be difficult. In this protocol, expression of a target protein in embryos is either knocked down using CRISPR-Cas9 technology (for zygotic proteins) or enhanced by microinjection of a synthetic mRNA (for maternal proteins). Western blotting analysis is then performed.

Information Accrual From the Period Preceding Racket-Ball Contact for Tennis Ground Strokes: Inferences From Stochastic Masking.

Previous research suggests the existence of an expert anticipatory advantage, whereby skilled sportspeople are able to predict an upcoming action by utilizing cues contained in their opponent's body kinematics. This ability is often inferred from "occlusion" experiments: information is systematically removed from first-person videos of an opponent, for example, by stopping a tennis video at the point of racket-ball contact, yet performance, such as discrimination of shot direction, remains above chance. In this study, we assessed the expert anticipatory advantage for tennis ground strokes via a modified approach, known as "bubbles," in which information is randomly removed from videos in each trial.

Permissive versus restrictive temperature thresholds in critically ill children with fever and infection: a multicentre randomized clinical pilot trial.

Background: Fever improves pathogen control at a significant metabolic cost. No randomized clinical trials (RCT) have compared fever treatment thresholds in critically ill children. We performed a pilot RCT to determine whether a definitive trial of a permissive approach to fever in comparison to current restrictive practice is feasible in critically ill children with suspected infection.

Different temperature thresholds for antipyretic intervention in critically ill children with fever due to infection: the FEVER feasibility RCT.

Background: Fever accelerates host immune system control of pathogens but at a high metabolic cost. The optimal approach to fever management and the optimal temperature thresholds used for treatment in critically ill children are unknown.

Objectives: To determine the feasibility of conducting a definitive randomised controlled trial (RCT) to evaluate the clinical effectiveness and cost-effectiveness of different temperature thresholds for antipyretic management.

Classification Videos Reveal the Visual Information Driving Complex Real-World Speeded Decisions.

Humans can rapidly discriminate complex scenarios as they unfold in real time, for example during law enforcement or, more prosaically, driving and sport. Such decision-making improves with experience, as new sources of information are exploited. For example, sports experts are able to predict the outcome of their opponent's next action (e.

A Roving Dual-Presentation Simultaneity-Judgment Task to Estimate the Point of Subjective Simultaneity.

The most popular tasks with which to investigate the perception of subjective synchrony are the temporal order judgment (TOJ) and the simultaneity judgment (SJ). Here, we discuss a complementary approach-a dual-presentation (2x) SJ task-and focus on appropriate analysis methods for a theoretically desirable "roving" design. Two stimulus pairs are presented on each trial and the observer must select the most synchronous.