IVSII-666 of human beta-globin gene: a polymorphic marker linked to codon 8(-AA) mutation.

Aims: IVSII-666 (C-T) is one of the polymorphic sites located in the second intron of the β-globin gene. Its polymorphism rate and relationship to a specific mutation are studied for the first time on 211 DNA samples of thalassemia trait patients living in Mazandaran province in North Iran using Ssp1 restriction enzyme. β-Globin haplotype determination at XmnI/(G)γ, HincII/3'Ψβ, HinfI/5'β, RsaI/5'β, and SspI/β sites was also performed by analysis of family members.