Optimization of ultraviolet/ozone (UVO) process conditions for the preparation of gelatin coated polystyrene (PS) microcarriers.

The aim of this study was to develop gelatin coated polystyrene (PS) microcarriers with good cell adhesion and proliferation properties. PS microspheres, prepared using oil-in water (o/w) solvent evaporation method, were loaded with oxygen containing functional groups using an ultraviolet/ozone (UVO) system. Using water-soluble carbodiimide chemistry, gelatin was subsequently immobilized on UVO treated PS microspheres.