miRNA-seq analysis revealed a potential strategy underlying poplar root responses to low nitrogen stress.

Main Conclusion: 87 miRNAs responding to low nitrogen stress in poplar roots were identified by miRNA-seq, and their target genes were predicted. Additionally, several key miRNA-mRNA modules were summarized.

Asbtract: Nitrogen (N) is an essential nutrient for plants, and low nitrogen (LN) availability can constrain plant growth and development.

Hydrogel immobilized bacteria@metal-organic-frameworks composite augmented bisphenol A removal from activated sludge and its regulation behavior on sludge community.

Improving degradation efficiency of activated sludge towards bisphenol A (BPA) is related to water safety. A hydrogel immobilized bacteria@metal-organic-frameworks (im-SQ-2@MOFs) was synthesized previously, which was a composite formed by metal organic frameworks adhering to BPA degrading bacteria. Accordingly, this study added im-SQ-2@MOFs as enhancer to augment the BPA degradation ability of activated sludge.

Epidemic status of echinococcosis, brucellosis, and tuberculosis among rural residents in three counties of Wuzhong City, Ningxia, China in 2023.

In recent years, the rapid development of animal husbandry in rural China has brought about the problem of zoonosis. In 2023, a cross-sectional study was carried out to gain an in-depth understanding of the infection status and influencing factors of three common zoonoses (echinococcosis, brucellosis and tuberculosis) among rural residents in three counties/districts of Wuzhong City, Ningxia. For the selection of the study participants, a cluster sampling approach was adopted.

Integration of comparative cytology, ionome, transcriptome and metabolome provide a basic framework for the response of foxtail millet to Cd stress.

Apart from directly affecting the growth and development of crops, Cd in the soil can easily enter the human body through the food chain and pose a threat to human health. Therefore, understanding the toxicity of Cd to specific crops and the molecular mechanisms of their response to Cd is essential. In this study, hydroponic experiments were utilized to study the response of foxtail millet to Cd stress through phenotypic investigation, enzyme activity determination, ultrastructure, ionome, transcriptome and metabolome.

Construction of Multifunctional Composite Films with Biomimetic Bridging and Stress Dissipation Properties.

Strategies based on biodegradable polymers to replace traditional petroleum-based plastics have become research hotspots, while designing multifunctional composite films as packaging materials remains a challenging task. Herein, mussel-inspired hollow mesoporous polydopamine (HMPDA) is prepared and used as a secondary reaction platform to immobilize silver nanoparticles (HMPDA@Ag), which are then co-doped into a polyvinyl alcohol (PVA) matrix in combination with naturally derived 2D montmorillonite (MMT). Benefiting from the noncovalent interactions between the composite particles and MMT with the PVA matrix, as well as the synergistic effects between nanofillers, the fabricated films exhibit a great tensile strength of 424.

NRF-1 promotes FUNDC1-mediated mitophagy as a protective mechanism against hypoxia-induced injury in cardiomyocytes.

Hypoxia-induced apoptosis and mitochondrial dysfunction in cardiomyocytes are involved in the mechanisms of heart failure. Our previous studies have confirmed that NRF-1 alleviates hypoxia-induced injury by promoting mitochondrial function and inhibiting apoptosis in cardiomyocytes. However, the mechanism by which NRF-1 attenuates hypoxia-induced injury in cardiomyocytes is still unclear.

FastCCC: A permutation-free framework for scalable, robust, and reference-based cell-cell communication analysis in single cell transcriptomics studies.

Detecting cell-cell communications (CCCs) in single-cell transcriptomics studies is fundamental for understanding the function of multicellular organisms. Here, we introduce FastCCC, a permutation-free framework that enables scalable, robust, and reference-based analysis for identifying critical CCCs and uncovering biological insights. FastCCC relies on fast Fourier transformation-based convolution to compute -values analytically without permutations, introduces a modular algebraic operation framework to capture a broad spectrum of CCC patterns, and can leverage atlas-scale single cell references to enhance CCC analysis on user-collected datasets.

Development and multicenter validation of machine learning models for predicting postoperative pulmonary complications after neurosurgery.

Background: Postoperative pulmonary complications (PPCs) are major adverse events in neurosurgical patients. This study aimed to develop and validate machine learning models predicting PPCs after neurosurgery.

Methods: PPCs were defined according to the European Perioperative Clinical Outcome standards as occurring within 7 postoperative days.

Buckwheat protein-derived peptide ameliorates insulin resistance by directing O-linked N-acetylglucosamine transferase to regulate the SIRT1/PGC1α pathway.

The antidiabetic activity of the novel Buckwheat protein-derived peptide (Ala-Phe-Tyr-Arg-Trp, AFYRW) and its associated protein glycosylation have been verified. Our preliminary study demonstrates the potential of AFYRW as a therapeutic agent for diabetes, but the mechanism needs further investigation. Given the vital role of O-linked N-acetylglucosamine transferase (OGT) in diabetes mellitus and insulin resistance (IR), we focused on the underlying molecular mechanisms of them in ameliorating IR.

Integrative Single-Cell and Bulk RNA Sequencing Identifies a Macrophage-Related Prognostic Signature for Predicting Prognosis and Therapy Responses in Colorectal Cancer.

Colorectal cancer (CRC) is one of the most common malignant tumors, characterized by a high incidence and mortality rate. Macrophages, as a key immune cell type within the tumor microenvironment (TME), play a key role in tumor immune evasion and the progression of CRC. Therefore, identifying macrophage biomarkers is of great significance for predicting the prognosis of CRC patients.

Emission of Protonated Riboflavin Induced by Inhibition of Out-of-plane Vibration in a Rigid Polymer Network.

Chromophores incorporated into rigid polymer matrices may exhibit novel photophysical properties distinct from those in liquid solutions. In this work, we explored the decay path of the second ππ* state (2ππ*) of riboflavin in poly(vinyl alcohol) (PVA) solutions and films with various acidities. Highly efficient internal conversion from 2ππ* to the lowest ππ* state (1ππ*) induced by slight in-plane motion is demonstrated in all PVA solutions and films, irrespective of environmental acidity and rigidification.

Walnut PR10/Bet v1-like proteins interact with chitinase in response to anthracnose stress.

Walnut is a significant woody oil tree species that has been increasingly affected by anthracnose in recent years. Effective anthracnose control is crucial for walnut yield and quality, which requires a comprehensive understanding of the response mechanisms to Colletotrichum gloeosporioides. The PR10/Bet v1-like proteins are involved in defense against various diseases, therefore, in this study, seven JrBet v1s were identified from the walnut transcriptome (named JrBet v1-1~1-7), whose open reading frame was 414~483 bp in length with isoelectric point ranging from 4.

Walnut phosphatase 2A proteins interact with basic leucine zipper protein JrVIP1 to regulate osmotic stress response calcium signaling.

Walnut is an important economic tree species that is susceptible to osmotic stress. Scientific cultivation management is an important way to improve the yield and quality of walnuts, which requires understanding the regulatory mechanisms in response to osmotic stress. Therefore, in this study, 15 protein phosphatase 2A () genes were identified from the walnut transcriptome (named ~) and their potential function responses to osmotic stress were elucidated.

Integrating dynamic high-throughput phenotyping and genetic analysis to monitor growth variation in foxtail millet.

Background: Foxtail millet [Setaria italica (L.) Beauv] is a C graminoid crop cultivated mainly in the arid and semiarid regions of China for more than 7000 years. Its grain highly nutritious and is rich in starch, protein, essential vitamins such as carotenoids, folate, and minerals.

A R2R3-MYB, BpMYB1, from paper mulberry interacts with DELLA protein BpGAI1 in soil cadmium phytoremediation.

Heavy metal pollution has become increasingly prominent, and bioremediation of heavy metal polluted areas is urgently needed. Broussonetia papyrifera is a pioneer tree species for vegetation restoration in the tailings area, while its molecular mechanism of heavy metal adaptation is not clear. Here, we report that a R2R3 MYB from B.

Over-expression of SiADCL1 in Arabidopsis modulates folate and amino acid metabolism to impact on flowering time.

Foxtail millet is a C crop rich in folate (FA). This study explores the roles of the 4-amino-4-deoxychorismate lyase (ADCL) - a member of the transaminase IV group of enzymes - in FA metabolism and conferred phenotypes. Phylogenetic comparisons identified diversity in the transaminase IV/ADCL gene family in the foxtail millet genome which was associated with genomic duplications.

Fluorescence Enhancement of Nonemissive Monodeprotonated Luteolin in a Poly(vinyl alcohol) Film.

Solid polymer matrixes can modulate the electronic states of embedded chromophores and have been widely used in flexible optoelectronic and optical materials. Luteolin is one of the most common natural flavonoids, and its neutral and monodeprotonated forms are nonemissive in aqueous solution induced by ultrafast excited-state proton transfer (ESPT) followed by nonradiative relaxation. In this study, we have incorporated luteolin into poly(vinyl alcohol) (PVA) films and studied their fluorescence behaviors.

New insights into uranium biomineralization mediated by Pseudomonas sp. WG2-6 in the presence of organic phosphorus: Promoting effect of extracellular polymeric substance and formation of U-P nanominerals.

Microbial biomineralization significantly affects the uranium (U) behavior in the environment. However, the mechanism of microbial biomineralization of U is still not fully understood. In this study, a dominant bacterium (Pseudomonas sp.

Identification of Novel Anoikis-Related Gene Signatures to Predict the Prognosis, Immune Microenvironment, and Drug Sensitivity of Breast Cancer Patients.

Introduction: Breast cancer is one of the most prevalent types of cancer and a leading cause of cancer-related death among females worldwide. Anoikis, a specific type of apoptosis that is triggered by the loss of anchoring between cells and the native extracellular matrix, plays a vital role in cancer invasion and metastasis. However, studies that focus on the prognostic values of anoikis-related genes (ARGs) in breast cancer are scarce.

Iron-based theranostic nanoenzyme for combined tumor magneto-photo thermotherapy and starvation therapy.

To address the issue of high-dose treatment agents in magnetic hyperthermia-mediated multi-model tumor therapy, a unique iron-based theranostic nanoenzyme with excellent magnetothermal and catalytic properties was constructed. By using a high-temperature arc method, the iron carbon nanoparticles (MF1-3) with a particle size between 13.7 and 27.

An integration of genome-wide survey, homologous comparison and gene expression analysis provides a basic framework for the ZRT, IRT-like protein (ZIP) in foxtail millet.

Essential mineral elements such as zinc and iron play a crucial role in maintaining crop growth and development, as well as ensuring human health. Foxtail millet is an ancient food crop rich in mineral elements and constitutes an important dietary supplement for nutrient-deficient populations. The ZIP (ZRT, IRT-like protein) transporters are primarily responsible for the absorption, transportation and accumulation of Zn, Fe and other metal ions in plants.

Enhancing the therapeutic potential of P29 protein-targeted monoclonal antibodies in the management of alveolar echinococcosis through CDC-mediated mechanisms.

Alveolar echinococcosis (AE) is a highly lethal helminth infection. Current chemotherapeutic strategies for AE primarily involve the use of benzimidazoles (BZs) such as mebendazole (MDZ) and albendazole (ABZ), which exhibit limited efficacy. In a previous study, the vaccine of recombinant Echinococcus granulosus P29 (rEgP29) showed significant immunoprotection against E.

Flexible/Regenerative Nanosensor with Automatic Sweat Collection for Cytokine Storm Biomarker Detection.

The real-time monitoring of low-concentration cytokines such as TNF-α in sweat can aid clinical physicians in assessing the severity of inflammation. The challenges associated with the collection and the presence of impurities can significantly impede the detection of proteins in sweat. This issue is addressed by incorporating a nanosphere array designed for automatic sweat transportation, coupled with a reusable sensor that employs a Nafion/aptamer-modified MoS field-effect transistor.

Targeting the mSWI/SNF complex in POU2F-POU2AF transcription factor-driven malignancies.

The POU2F3-POU2AF2/3 transcription factor complex is the master regulator of the tuft cell lineage and tuft cell-like small cell lung cancer (SCLC). Here, we identify a specific dependence of the POU2F3 molecular subtype of SCLC (SCLC-P) on the activity of the mammalian switch/sucrose non-fermentable (mSWI/SNF) chromatin remodeling complex. Treatment of SCLC-P cells with a proteolysis targeting chimera (PROTAC) degrader of mSWI/SNF ATPases evicts POU2F3 and its coactivators from chromatin and attenuates downstream signaling.